Current Genetics

, Volume 13, Issue 6, pp 471–478 | Cite as

Cloning, sequencing and analysis of the yeastS. uvarum ERG10 gene encoding acetoacetyl CoA thiolase

  • Sylvie Dequin
  • Remi Gloeckler
  • Christopher J. Herberte
  • Françoise Boutelet
Original Articles

Summary

TheERG10 gene specific toS. uvarum, a brewing yeast, has been cloned by complementation of anS. cerevisiae erg10 mutant.S. uvarum contains two differentERG10 genes. One of these is similar to theS. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The clonedERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolatedtsm0115 mutants. Northern blot and sequence analysis indicate that theERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase.

Key words

Ergosterol Biosynthesis Saccharomyces uvarum Regulation 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Sylvie Dequin
    • 1
  • Remi Gloeckler
    • 1
  • Christopher J. Herberte
    • 2
  • Françoise Boutelet
    • 1
  1. 1.TEPRAL S.A.StrasbourgFrance
  2. 2.Centre de Génétique Moléculaire du CNRSLaboratoire propre associé à l’Université Pierre et Marie CurieGif-sur YvetteFrance

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