Current Genetics

, Volume 13, Issue 6, pp 471–478 | Cite as

Cloning, sequencing and analysis of the yeastS. uvarum ERG10 gene encoding acetoacetyl CoA thiolase

  • Sylvie Dequin
  • Remi Gloeckler
  • Christopher J. Herberte
  • Françoise Boutelet
Original Articles


TheERG10 gene specific toS. uvarum, a brewing yeast, has been cloned by complementation of anS. cerevisiae erg10 mutant.S. uvarum contains two differentERG10 genes. One of these is similar to theS. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The clonedERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolatedtsm0115 mutants. Northern blot and sequence analysis indicate that theERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase.

Key words

Ergosterol Biosynthesis Saccharomyces uvarum Regulation 


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  1. Bennetzen JL, Hall BD (1982) J Biol Chem 257:3026–3031PubMedGoogle Scholar
  2. Boutelet F (1982) PhD thesis, University of Gembloux, BelgiumGoogle Scholar
  3. Carle GF, Olson MV (1984) Nucleic Acids Res 12:5647–5664PubMedGoogle Scholar
  4. Carlson M, Botstein D (1982) Cell 28:145–154PubMedCrossRefGoogle Scholar
  5. Casey GP (1986) Carlsberg Res Commun 51:327–341Google Scholar
  6. Cohen SN, Chang ACY, Hsu L (1972) Proc Natl Acad Sci USA 69:2110–2114PubMedCrossRefGoogle Scholar
  7. Dale RMK, McLure BA, Houchins JP (1985) Plasmid 13:31–40PubMedCrossRefGoogle Scholar
  8. Dobson MJ, Tuite MF, Roberts NA, Kingsman AJ, Kingsman SM (1982) Nucleic Acids Res 10:2625–2637PubMedGoogle Scholar
  9. Gehring U, Harris JI (1970) Eur J Biochem 16:492–498PubMedCrossRefGoogle Scholar
  10. Gerbaud C, Elmerich C, Tandeau de marsac N, Chocat P, Charpin N, Guerineau M, Auber JP (1981) Curr Genet 3:173–181CrossRefGoogle Scholar
  11. Hahn S, Hoar ET, Guarente L (1985) Proc Natl Acad Sci USA 82:8562–8566PubMedCrossRefGoogle Scholar
  12. Hicks JB, Strathern JN, Klar ASJ, Dellaporta SL (1982) In: Setlow JK, Hollaender A (eds) Genetic engineering USA 4: 219–248Google Scholar
  13. Hilger F, Prevot M, Mortimer R (1982) Curr Genet 6:93–98CrossRefGoogle Scholar
  14. Holmberg S (1982) Carlsberg Res Commun 47:233–244Google Scholar
  15. Hurt EC, Van Loon APGM (1986) TIBS II:204–207Google Scholar
  16. Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168PubMedGoogle Scholar
  17. Kornblatt JA, Rudney H (1971a) J Biol Chem 246:4417–4423PubMedGoogle Scholar
  18. Kornblatt JA, Rudney H (1971b) J Biol Chem 246:4424–4430PubMedGoogle Scholar
  19. Maniatis T, Fritsh EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
  20. Messing J (1983) In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, vol 101. Academic Press, New York, pp 20–49Google Scholar
  21. Myers AM, Tzagoloff A (1985) J Biol Chem 260:15371–15377PubMedGoogle Scholar
  22. Natsoulis G, Hilger F, Fink GR (1986) Cell 46:235–243PubMedCrossRefGoogle Scholar
  23. Naumovski L, Friedberg EC (1983) Gene 22:203–209PubMedCrossRefGoogle Scholar
  24. Nilsson-Tillgren T, Gjermansen C, Holmberg S, Petersen JGL (1986) Carlsberg Res Commun 51:309–326Google Scholar
  25. Pedersen MB (1985) Carlsberg Res Commun 180:263–272Google Scholar
  26. Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467PubMedCrossRefGoogle Scholar
  27. Schleif RF, Wensink PC (1981) In: Pract Meth in Mol Biol, vol 74. Springer, Vienna New YorkGoogle Scholar
  28. Servouse M, Mons N, Baillargeat JL, Karst F (1984) Biochem Biophys Res Commun 123:424–430PubMedCrossRefGoogle Scholar
  29. Servouse M, Karst F (1986) Biochem J 240:541–547PubMedGoogle Scholar
  30. Southern EM (1975) J Mol Biol 98:503–517PubMedGoogle Scholar
  31. Stewart PR, Rudney H (1966) J Biol Chem 241:1212–1221PubMedGoogle Scholar
  32. Trocha PJ, Sprinson DB (1976) Arch Biochem Biophys 174:45–51PubMedCrossRefGoogle Scholar
  33. Van Soligen P, Van der Plaat JB (1977) J Bacteriol 130:946–947Google Scholar
  34. Wickner RB, Boutelet F, Hilger F (1983) Mol Cell Biol 3:415–420PubMedGoogle Scholar
  35. Zaret KS, Sherman F (1982) Cell 28:563–573PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Sylvie Dequin
    • 1
  • Remi Gloeckler
    • 1
  • Christopher J. Herberte
    • 2
  • Françoise Boutelet
    • 1
  1. 1.TEPRAL S.A.StrasbourgFrance
  2. 2.Centre de Génétique Moléculaire du CNRSLaboratoire propre associé à l’Université Pierre et Marie CurieGif-sur YvetteFrance

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