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European Biophysics Journal

, Volume 18, Issue 3, pp 159–164 | Cite as

Influence of net charge on the aggregation and solubility behaviour of amphotericin B and its derivatives in aqueous media

  • J. Mazerski
  • J. Grzybowska
  • E. Borowski
Article

Abstract

The poor solubility of polyene antibiotics in aqueous media limits their application in the therapy of systemic fungal infections. In the present paper we have demonstrated that the ionic state (net electrical charge) of the antibiotic molecule is an important factor in determining the aggregation and solubility properties of amphotericin B and its derivatives. A multi-step model of polyene self-association in aqueous media has been proposed as an explanation for the fact that some major differences are observed when aggregation is monitored by different techniques.

Key words

Amphotericin B aggregation solubility aqueous media mathematical model 

Abbreviations

DMF

dimethylformamide

DMSO

dimethylsulphoxide

CD

circular dichroism

MMC

maximal monomer concentration

MSC

maximal solute concentration

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References

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • J. Mazerski
    • 1
  • J. Grzybowska
    • 1
  • E. Borowski
    • 1
  1. 1.Department of Pharmaceutical Technology and BiochemistryTechnical University of GdańskGdańskPoland

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