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Biochemische untersuchungen über die entwicklung des stoffwechsels in der rattennetzhaut

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Zusammenfassung

Bei der Messung von Atmung and Glykolyse der Rattennetzhaut zeigt sich vom 10. bis zum 30. Lebenstag ein kontinuierlicher Anstieg. Gleiches gilt für die Enzyme Malatdehydrogenase and Glycerinaldehyd-phosphat-Dehydrogenase. Die Zellzahl — gemessen am DNS-Gehalt —bleibt jedoch konstant. Die Befunde weisen daher auf eine echte Stoffwechselsteigerung hin. Die Ergebnisse werden zu der morphologischen and funktionellen Ausdifferenzierung der Netzhaut in diesem Zeitraum in Beziehung gesetzt.

Summary

An analysis of oxygen consumption and glycolysis in the isolated rat retina shows a continuous increase from the 10th to 30th day. Similarly the activity of glyceraldehyde-3-phosphate dehydrogenase and malic dehydrogenase is increased. On the other hand the cell concentration—as determined by DNA content-remains constant. These findings suggest a real increase of metabolic activity. The results are correlated to the morphological and functional differentiation of the retina during this period.

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Literatur

  • Ballou, C. E., andH. O. L Fischer: The synthesis of D-glyceraldehyd-3-phosphate. J. Amer. chem. Sec.77, 3329 (1955).

    Article  CAS  Google Scholar 

  • Berkow, J. W., andA. Patz: Developmental histochemistry of the rat eye. Invest. Ophthal.3, 22 (1964)

    PubMed  CAS  Google Scholar 

  • Bonavita, V., F. Ponte, andG. Amore: Neurochemical studies on the inherited retinal degeneration of the rat. Vision Res.3, 271 (1963).

    Article  CAS  Google Scholar 

  • Bonting, S. L., L. L. Caravaggio, andP. Gouras: The rhodopsin cycle in the developing vertebrate retina. Exp. Eye Res.1, 14 (1961).

    PubMed  CAS  Google Scholar 

  • Brotherton, J.: Studies on the metabolism of the rat retina with special reference to retinitis pigmentosa. Exp. Eye Res.1, 234 (1962).

    PubMed  CAS  Google Scholar 

  • Cohen, L. H., andW. K. Noell: Glucose catabolism of rabbit retina before and after development of visual function. J. Neurochem.5, 253 (1960).

    PubMed  CAS  Google Scholar 

  • De Robertis, E., andA. Lasansky: Ultrastructure and chemical organization of photoreceptors. In: George K. Sdzelser (ed.), The structure of the eye, S. 29. New York: Academic Press, Inc. 1961.

    Google Scholar 

  • Detwiler, S. R.: Experimental observations on the developing rat retina. J. comp. Neurol.55, 473 (1932).

    Article  Google Scholar 

  • Dowling, J. E., andR. L. Sidman: Inherited retinal dystrophy in the rat. J. Cell Biol.14, 73 (1962).

    Article  PubMed  CAS  Google Scholar 

  • Forgacs, J.: Activité de la glyceraldehyde-3-phosphate déhhydrogénase dans la rétine du lapin et dans la rétinite pigmentaire expérimentale provoquée par le monoidacétate. Etude histochimique. Experientia (Basel)19, 236 (1963).

    CAS  Google Scholar 

  • Graymore, C.: (1) Metabolism of the developing retina. 1. Aerobic and anaerobic glycolysis in the developing rat retina. Brit. J. Ophthal.43, 34 (1959).

    PubMed  CAS  Google Scholar 

  • — (2) Metabolism of the developing retina. III. Respiration in the developing normal rat retina and the effect of an inherited degeneration of the retinal neuroepithelium. Brit. J. Ophthal.44, 363 (1960).

    PubMed  CAS  Google Scholar 

  • — (3) Possible significance of the isoenzymes of lactic dehydrogenase in the retina of the rat. Nature (Lond.) 201, 615 (1964).

    CAS  Google Scholar 

  • — andM. Towlson: Levels of soluble NAD-linked a-glycerophosphate dehydrogenase and lactic acid dehydrogenase in rat retina. Exp. Eye Res.2, 48 (1963).

    PubMed  CAS  Google Scholar 

  • Hellner, K. A., u.D. Utermann: Quantitative elektroretinographische Untersuchungen über die Entwicklung der normalen and dystrophischen Battennetzhaut. Vision Res. (im Druck.)

  • Hilz, H., C. Erich u.D. Glaubitt: Verdnderung von Zelldichte und Polysaccharidstoffwechsel im alternden Bindegewebe. Klin. Wschr.41, 332 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Keck, K.: An ultramicro technique for the determination of deoxypentose nucleic acid. Arch. Biochem.63, 446 (1956).

    Article  PubMed  CAS  Google Scholar 

  • Krebs, H. A.: Body size and tissue respiration. Biochim. biophys. Acta (Amst.)4, 249 (1950).

    Article  CAS  Google Scholar 

  • Lowry, O. H., N. R. Roberts, andC. Lewis: The quantitative histochemistry of the retina. J. biol. Chem.220, 879 (1956).

    PubMed  CAS  Google Scholar 

  • Nemeth, A. M.: Mechanisms controlling changes in tryptophan peroxidase activity in developing mammalian liver. J. biol. Chem.234, 2921 (1959).

    PubMed  CAS  Google Scholar 

  • Niemi, M., andE. Merenmses: Cytochemical localization of the oxydative enzyme systems in the retina. I. J. Neurochem.6, 200 (1961).

    PubMed  CAS  Google Scholar 

  • Pearse, A. G. Everson: Localization of oxydative enzymes in rat and chick retina in various physiological conditions. In:George K. Smelser (ed.), The structure of the eye, S. 53. New York: Academic Press, Inc. 1961.

    Google Scholar 

  • Schimke, R. T.: Histochemical determinations of glyceraldehyd phosphate and glycerophosphate dehydrogenase in brain. Fed. Proc.16, 1428 (1957).

    Google Scholar 

  • Umbreit, W. W., R. H. Burms, andJ. F. Stauffer: Manometric techniques, S. 149. Minneapolis: Burgess Publ. Co. 1957.

    Google Scholar 

  • Walters, P. T.: Anaerobic glycolysis in rats affected with retinitis pigmentosa. Brit. J. Ophthal.43, 686 (1959).

    PubMed  CAS  Google Scholar 

  • Warburg, O.: Über die Klassifizierung tierischer Gewebe nach ihrem Stoffwechsel. Biochem. Z.184, 484 (1927).

    CAS  Google Scholar 

  • —, u.W. Christian: Isolierung und Kristallisation des Proteins des oxydierenden Gärungsfermentes. Biochem. Z.303, 40 (1939).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Universitäts-Augenklinik Hamburg-Eppendorf, Germany

    Dieter Utermann

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  1. Dieter Utermann
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Utermann, D. Biochemische untersuchungen über die entwicklung des stoffwechsels in der rattennetzhaut. Graefes Arhiv Fur Ophthalmologie 167, 541–548 (1964). https://doi.org/10.1007/BF02426617

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