Biophysics of structure and mechanism

, Volume 4, Issue 3, pp 209–222 | Cite as

Tension transients in fibrillar muscle fibres as affected by stretch-dependent binding of AMP-PNP: A teinochemical effect?

  • H. J. Kuhn
Article

Abstract

The recovery in tension after release of a fibrillar muscle preparation as well as the fall in tension after restretch was found to be greater in presence of AMP-PNP than in its absence (rigor). The effect of AMP-PNP was concentration-dependent with an optimum at 0.1 mM corresponding to the dissociation constant of AMP-PNP from the myosin heads. This evidence supports the validity of the teinochemical principle which predicts a stretch-dependent AMP-PNP binding. The stiffness calculated per cross bridge was similar to that found by Huxley and Simmons (1971). It was further calculated that only 15% of the cross bridges are in a force-maintaining state in rigor.

Key words

ATP analogue Insect fibrillar muscle Stiffness per cross bridge Quick release-recovery Mechano-chemistry of muscle 

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • H. J. Kuhn
    • 1
  1. 1.Department of Physiology IIUniversity of HeidelbergHeidelbergFederal Republic of Germany

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