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“Minisatellite” DNA probes detect engraftment and/or chimerism in recipients of HLA-matched bone marrow transplants

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Transplant International

Abstract

A study was carried out to determine whether the minisatellite DNA probes described by Jeffreys and coworkers could be used routinely to analyze engraftment, hematopoietic chimerism, and relapse in recipients of bone marrow transplants. The probes were informative for all of the recipient/donor pairs analyzed. Their limit of sensitivity was determined in reconstruction experiments and was found to vary from 2%, in the best cases, to 10%. We were able to confirm that engraftment and hematopoietic chimerism can, indeed, be sought routinely using this simple molecular approach. Only one set of probes is required for all patients and, unlike cytogenetic analysis, this analysis can be used whether or not blood or marrow cells are dividing.

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Authors and Affiliations

  1. Unité 119 de l'Institut National de la Santé et de la Recherche Médicale, Institut Paoli-Calmettes, 27, Boulevard Lei Roure, F-13009, Marseille, France

    Luc Xerri, Claude Mawas & Françoise Birg

  2. Unité de Transplantation Médullaire, Institut Paoli-Calmettes, 27, Boulevard Lei Roure, F-13009, Marseille, France

    Dominique Maraninchi & Marie-Hélène Gaspard

  3. Laboratoire de Cytogénétique et d'Hématologie, CHR de Saint Etienne Nord, F-42277, Saint-Priest-en-Jarez

    Marie-Françoise Bertheas

Authors
  1. Luc Xerri
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  2. Dominique Maraninchi
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  3. Marie-Françoise Bertheas
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  4. Marie-Hélène Gaspard
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  5. Claude Mawas
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  6. Françoise Birg
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Xerri, L., Maraninchi, D., Bertheas, MF. et al. “Minisatellite” DNA probes detect engraftment and/or chimerism in recipients of HLA-matched bone marrow transplants. Transplant Int 2, 53–55 (1989). https://doi.org/10.1007/BF02425974

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  • DOI: https://doi.org/10.1007/BF02425974

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