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Formation of merodiploid clones by conjugation inRhizobium lupini

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Summary

Off the transconjugants formed in theR. lupini conjugation 0.5 to 5% are merodiploids. When two differently pigmented parents are used in the crossing experiment the diploid transconjugants can be differentiated from the haploid recombinants by their additive pigmentation type. The segregation patterns of these diploid clones were analyzed. The results are in agreement with the theory that the exogenotic donor DNA can be integrated at different sites of the homologous recipient chromosomal region forming a tandem sequence. Consequently the segregants of these merodiploid clones are formed by endochromosomal recombination.

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Erlangen-Nürnberg, D-8520, Erlangen, Federal Republic of Germany

    W. Heumann & R. Springer

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  1. W. Heumann
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  2. R. Springer
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Communicated by F. Kaudewitz

This work was supported by the Deutsche Forschungsgemeinschaft and Stiftung Volkswagenwerk.

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Heumann, W., Springer, R. Formation of merodiploid clones by conjugation inRhizobium lupini . Molec. Gen. Genet. 150, 73–79 (1977). https://doi.org/10.1007/BF02425327

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  • DOI: https://doi.org/10.1007/BF02425327

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