Abstract
Meiotic recombination is not random in the proximal region of the mouse major histocompatibility complex (MHC). It is clustered at four restricted positions, so-called hotspots. Some of the MHC haplotypes derived from Asian wild mice enhance recombination at the hotspots in genetic crosses with standard MHC haplotypes of laboratory mouse strains. In particular, the wm7 haplotype derived from Japanese wild mouse indicated an approximately 2% recombination frequency within a 1.2 kb fragment of DNA in the interval between the Pb and Ob genes. Interestingly, this enhancement of recombination was observed only in female meiosis but not in male meiosis. Mating experiments demonstrated that the wm7 haplotype carries a genetic factor in the region proximal to the hotspot, which instigates recombination. In addition, the wm7 haplotype has a genetic factor located in the region distal to the hotspot, which suppresses recombination. From the molecular characterization of the two hotspots located in the Eb gene and the Pb-Ob interval, it appeared that there are several common molecular elements, the consensus of the middle repetitive MT-family, TCTG or CCTG tetramer repeats, and the solitary long terminal repeat (LTR) of mouse retrovirus.
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Benger, J., I. Teshima, M. A. Walter, M. G. Brubacher, G. Daouk & D. W. Cox, 1991. Localization and genetic linkage of the human immunoglobulin heavy chain genes and the creatine kinase brain (CKB) gene: Identification of a hotspot for recombination. Genomics 9: 614–622.
Bryda, E. C., J. A. Depari, D. B. Sant'Angelo & H. C. Passmore, 1992. Multiple sites of crossing over within the Eb recombinational hotspot in the mouse. Mammalian Genome 2: 123–129.
Chakravarti, A., S. C. Elbein & M. A. Permutt, 1986. Evidence for increased recombination near the human insulin gene: Implication for desease association studies. Proc. Natl. Acad. Sci. USA 83: 1045–1049.
Davisson, M. T., T. H. Roderick & D. P. Doolittle, 1989. Recombination percentages and chromosomal assignments. pp. 432–505. in Genetic variants and strains of the laboratory mouse. Second Edition. edited by M. F. Lyon & A. G. Searle. Oxford University Press, Oxford.
Donis-Keller, T. K., P. Green, C. Helms, S. Cartinhour, B. Weinffenbach, K. Stephen, T. P. Keit, D. W. Bowden, D. R. Smith, E. S. Lander, D. Botstein, G. Akots, K. S. Rediker, T. Gravius, V. A. Brown, M. B. Rising, C. Parker, J. A. Powers, D. E. Watt, E. K. Kauffman, A. Bricker, P. Phipps, H. Muller-Kahle, T. R. Fulton, S. Ng, J. W. Schumm, J. C. Braman, R. G. Knowlton, D. F. Barker, S. M. Crooks, S. E. Lincoln, M. J. Daly & J. Abrahamson, 1987. A genetic linkage map of the human genome. Cell 51: 319–337.
Edelman, W., B. Kroger, M. Goller & I. Horak, 1989. A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system. Cell 57: 937–946.
Fischer-Lindahl, K., B. Hausmann & V. M. Chapman, 1983. A new H-2 linked class I gene whose expression depends on a maternally inherited factor. Nature 306: 383–385.
Fischer-Lindahl, K., 1991. His and hers recombinational hotspots. Trends in Genet. 7: 273–276.
Heinlein, U. A. O., R. Lange-Stablitz, H. Schaal & W. Wille, 1986. Molecular characterization of the MT-family of dispersed middle-repetitive DNA in rodent genome. Nucl. Acids. Res. 14: 6403–6416.
Janson, M., C. Larsson, B. Werelius, C. Jones, T. Glaser, U. Nakamura & M. Nordenskjold, 1991. Detailed physical map of human chromosomal region 11q12-13 shows high meiotic recombination rate around theMEN1 locus. Proc. Natl. Acad. Sci. USA. 88: 10609–10613.
Klein, J., 1975. Biology of the mouse histocompatibility-2 complex. Springer-Verlag, Berlin, Heiderberg, New York.
Klein, J., 1986. Natural history of the major histocompatibility complex. A Wiley-Interscience Publications, New York.
Kobori, J. A., E. Strauss, K. Minard & L. Hood, 1986. Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science 234: 173–179.
Lafuse, W. P., S. Savarirayan & C. S. David, 1986. Mapping a second recombinational hotspot within the I-E region of the mouse H-2 gene complex.
Lowell, C. A., D. A. Potter, R. S. Stearman & J. F. Morrow, 1986. Structure of the murine serum amyloid A gene family. J. Bio. Chem. 261: 8442–8452.
Padgett, K. A., D. C. Shreffler & B. K. Saha, 1991. Molecular mapping of murine I region recombinants. III Crossing over at two discrete sites within the b1–b2 intron of the Eb gene. J. Immunol. 147: 2764–2770.
Radman, M., 1989. Mismatch repair and the fidelity of genetic recombination. Genome 31: 68–73.
Reeves, R. H., M. R. Crowley, B. F. Ohara & J. D. Gearhart, 1990. Sex, strain, and species differences affect recombination across an evolutionary conserved segment of mouse chromosome 16. Genomics 8: 141–148.
Shiroishi, T., T. Sagai & K. Moriwaki, 1982. A new wild-derived H-2 haplotype enhancing K-IA recombination. Nature 300: 370–372.
Shiroishi, T., T. Sagai & K. Moriwaki, 1987. Sexual preference of meiotic recombination within the H-2 complex. Immunogenet. 25: 258–262.
Shiroishi, T., N. Hanzawa, T. Sagai, M. Ishiura, T. Gojobori, M. Steinmetz & K. Moriwaki, 1990. Recombinational hotspot specific to female meiosis in the mouse major histocompatibility complex. Immunogenet. 31: 79–88.
Shiroishi, T., T. Sagai, N. Hanzawa, H. Gotoh & K. Moriwaki, 1991. Genetic control of sex-dependent meiotic recombination in the major histocompatibility complex of the mouse. EMBO J 10: 681–686.
Smith, G. R., 1983. General recombination pp. 175–209 in Lambda II, edited by R. W. Hendrix, J. W. Roberts, F. W. Stahl, and R. A. Weisberg. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Steinmetz, M., K. Minard, S. Hoevath, J. McNicholas, J. Frelinger, C. Wake, E. Long, B. Mach & L. Hood, 1982. A molecular map of the immune response region from the major histocompatibility complex. Nature 300: 35–42.
Steinmetz, M., D. Stephan & K. Fischer-Lindahl, 1986. Gene organization and recombinational hotspot in the murine major histocompatibility complex. Cell 44: 895–904.
Steinmetz, M., Y. Uematsu & K. Fischer-Lindahl, 1987. Hotspots of homologous recombination in mammalian genomes. Trends in Genet. 3: 7–10.
Sylla, B. S., D. Allard, G. Roy, D. Bourgaux-Ramoisy & P. Bourgaux, 1984. A mouse DNA sequence that mediates integration and excision of polyoma virus DNA. Gene 29: 343–350.
Thomas, B. J. & R. Rothstein, 1991. Sex, maps, and imprinting. Cell 64: 1–3.
Treco, D., B. Thomas & N. Arnheim, 1985. Recombination hotspot in the human b-globin gene cluster: Meiotic recombination of human DNA fragments in Saccharomyces cerevisiae. Mol. Cell. Biol. 5: 2029–2038.
Uematsu, Y., H. Kiefer, R. Schultz, K. Fischer-Lindahl & M. Steinmetz, 1986. Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing over. EMBO J 5: 2123–2129.
Wallenburg, J. C., A. Nepveu & P. Chartrand, 1984. Random and nonrandom integration of a polyoma DNA molecule containing highly repetitive cellular sequences. J. Virol. 50: 678–683.
Zimmerer, E. J. & H. C. Passmore, 1991. Structural and genetic properties of theEb recombinational hotspot in the mouse. Immunogenet. 33: 132–140.
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Shiroishi, T., Sagai, T. & Moriwaki, K. Hotspots of meiotic recombination in the mouse major histocompatibility complex. Genetica 88, 187–196 (1993). https://doi.org/10.1007/BF02424475
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DOI: https://doi.org/10.1007/BF02424475