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Genetica

, 67:51 | Cite as

Localisation of NORs and counterstain-enhanced fluorescence studies inPerca fluviatilis (Pisces, Percidae)

  • B. Mayr
  • P. Rab
  • M. Kalat
Article

Abstract

The karyotype of the perch (Percafluviatilis L.) was analysed by means of silver-staining, the chromomyc in A/3 Distamycin A/DAPI and Actinomycin D fluorescence technique in order to locate active NORs and to selectively highlight certain heterochromatic regions. The ribosomal RNA genes are localized at the secondary constrictions of the short arms of chromosomes no. 16. Additionally, bright chromomycin fluorescence was observed in the same regions. No DAPI/ Distamycin A bright heterochromatic block was detected in the perch karyotype.

Keywords

DAPI Actinomycin Fluorescence Technique Heterochromatic Region Fluorescence Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Abe, S. & Muramoto, J., 1977. Differential staining of chromosomes of two salmonid species. Salvelinus leucomaenis (Pallas) and Salvelinus malma (Waldbaum). Proc. Japan Acad. 50:507–511.Google Scholar
  2. Alvarez, M. C., Cano, J. & Thode, G., 1980. DNA content and chromosome complement of Chromis chromis (Pomacentridae, Perciformes). Caryologia 33: 267–274.Google Scholar
  3. Appels, R., 1982. The molecular cytology of wheat-rye hybrids. Int. Rev. Cytol. 80: 93–132.CrossRefGoogle Scholar
  4. Behr, W., Honikel, K. & Hartmann, G., 1969. Interaction of the RNA polymerase inhibitor chromomycin with DNA. Europ. J. Biochem. 9: 82–92.CrossRefPubMedGoogle Scholar
  5. Brown, D. D. & Weber, C. S., 1968. Gene linkage by RNA-DNA hybridisation. Unique DNA sequences homologous to 4S RNA, 5S DNA and ribosomal RNA. J. molec. Biol. 34: 661–680.CrossRefPubMedGoogle Scholar
  6. Deumling, B. & Greilhuber, J., 1982. Characterization of heterochromatin in different species of the Scilla siberica group (Liliaceae) by in situ hybridization of satellite DNAs and fluorochrome banding. Chromosoma 84: 535–555.CrossRefGoogle Scholar
  7. Foresti, F. Almeida-Toledo, L. F. & Toledo, S. A., 1981. Poly-morphic nature of nucleolus organizer regions in fishes. Cytogenet. Cell Genet. 31: 137–144.PubMedGoogle Scholar
  8. Goodpasture, C. & Bloom, S. E., 1975. Visualisation of nucleolus organizer regions in mammalian chromosomes using silver-staining. Chromosoma 55: 37–50.CrossRefGoogle Scholar
  9. Haaf, D. & Schmid, M., 1984. An early stage of ZW/ZZ sex chromosome differentiation in Poecillia sphenops var. melanistica (Poecilidae, Cyprinodotiformes). Chromosoma 89: 37–41.CrossRefGoogle Scholar
  10. Howell, W. M. & Bloom, S. E., 1973. Sex associated differential fluorescence of mudminnow chromosomes and spermatozoa. Nature 245: 261–263.CrossRefPubMedGoogle Scholar
  11. Howell, W. M., Denton, T. E.& Diamond, J. R., 1975. Differential staining of the satellite regions of human acrocentric chromosomes. Experientia 31: 260–262.CrossRefPubMedGoogle Scholar
  12. Jorgenson, K. F., van de Sande, J. H. & Lin, C. C., 1978. The use of base pair specific DNA-binding agents as affinity labels for the study of mammalian chromosomes. Chromosoma 68: 287–302.CrossRefPubMedGoogle Scholar
  13. Kligerman, A. D. & Bloom, S. E., 1977. Distribution of F-bodies, heterochromatin and nucleolar organizers in the genome of the central mudminnow. Umbra limi. Cytogenet. Cell Genet. 18: 182–196.PubMedGoogle Scholar
  14. Kodama, Y., Yoshida, M. C. & Sasaki, M., 1980. An improved silver staining technique for Nucleolus organizer regions by using nylon cloth. Jpn. J. Human Genet. 25: 229–233.CrossRefGoogle Scholar
  15. Mayr, B., Schweizer, D. & Schleger, W., 1983. Characterisation of the canine caryotype by counterstain-enhanced chromosome banding. Can. J. Genet. Cytol. 25: 616–621.PubMedGoogle Scholar
  16. Mayr, B., Schweizer, D. & Geber, G., 1984. NOR activity, heterochromatin differentiation and the Robertsonian polymorphism in Sus scrofa L. J. Hered. 75: 79–80.PubMedGoogle Scholar
  17. Nygren, A., Edlund, P., Hirsch, U. & Ashgren, L., 1968. Cytologic study in perch (Perca fluvialis L.). pike(Esox lucius L.), pike-perch (Lucioperca lucioperca L.) and ruff (Acerina cernka L.). Hereditas 59: 518–524.CrossRefGoogle Scholar
  18. Ojima, Y. & Ueda, T., 1979. New C-banded marker chromosomes found in carp-funa hybrids. Proc. Jap. Acad. Ser. B, 54: 15–20.Google Scholar
  19. Ojima, Y. & Takai, A., 1979. Further cytogenetic studies on the origin of the gold fish. Proc. Japan Acad. Ser. B 55: 346–350.Google Scholar
  20. Ojima, Y. & Yamano, T., 1980. The assignment of the nucleolar organizers in the chromosomes of the funa (Carrasius, Cyprinidae, Pisces). Proc Japan Acad. 56, Ser. B., No. 9: 551–556.Google Scholar
  21. Phillips, R. B. & Zajicek, K. D., 1982. Q-band chromosomal polymorphisms in lake trout (Salvelinus namaycush). Genetics 101: 227.PubMedGoogle Scholar
  22. Rab, P., 1981. Karyotype of European catfish Silurus glanis L. (Pisces. Siluridae Pisces), with remarks on the cytogenetics of siluroid fishes. Folia Zoologica 30(3): 271–286.Google Scholar
  23. Rab, P. & Liebman, P., 1982. Chromosomes study of the Danube Salmon Hucho Hucho (Pisces, Salmonidae). Folia Zoologica. 31: 181–190.Google Scholar
  24. Rab. P., 1982. Karyotypes of six specimens of Europea percid fishes (Percidae, Pisces). Abstr. Eur. Ichtyol. Confer. No. 243. Hamburg, 243.Google Scholar
  25. Sahar, E. & Latt, S. A., 1978. Enhancement of banding patterns in human chromosomes by energy transfer. Proc. natn. Acad. Sci. (USA) 75: 5650–5654.Google Scholar
  26. Schnedl, W., Breitenbach, M., Mikelsaar, A. V. & Stranzinger, G., 1977. Mithramycin and DIPI: a pair of fluorochromes specific for GC- and AT-rich DNA respectively. Hum. Genet. 36: 299–305.CrossRefPubMedGoogle Scholar
  27. Schmid, M., 1980. Chromosome banding in Amphibia. IV. Differentiation of GC and AT-rich chromosome regions in Anura. Chromosoma (Berl.) 77: 83–103.CrossRefGoogle Scholar
  28. Schweizer, D., 1976. Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma (Berl.) 58: 307–327.CrossRefGoogle Scholar
  29. Schweizer, D., 1980. Simultaneous fluorescent staining of R-bands and specific heterochromatin regions (DA/ DAPIbands) in human chromosomes. Cytogenet. Cell Genet. 27: 190–193.PubMedGoogle Scholar
  30. Schweizer, D., 1981. Counterstain-enhanced chromosome banding. Hum. Genet. 57: 1–14.PubMedGoogle Scholar
  31. Schweizer, D., Mendelak, M., White, M. J. D. & Contreras, N., 1983. Cytogenetics of the parthenogenetic grasshopper Warramaba virgo and its bisexual relatives. X. Patterns of fluorescent banding. Chromosoma 88: 227–236.CrossRefGoogle Scholar
  32. Sinclair, J. H. & Brown, D. D., 1971. Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of some eukaryotes and some of their physical characteristics. Biochemistry 10: 2761–2769.CrossRefPubMedGoogle Scholar
  33. Takai, A. & Ojima, Y., 1982. The assignment on the nucleolus organizer regions in the chromosomes of the Carp. the Funa and their hybrids (Cyprinidae, Pisces). Proc. of the Japan Acad. 58, Ser. B: 303.Google Scholar
  34. Thode, G., Cano, J. & Alvarez, M. C., 1983. Association of nucleolus organizer chromosomes shown by silver staining in Gobius fallax. J. Hered. 74: 480–482.Google Scholar
  35. Thorgaard, G. H., 1976. Robertsonian polymorphism and constitutive heterochromatin distribution in chromosomes of the rainbow trout (Salmo gairdneri). Cytogenet. Cell Genet. 17:174–184.PubMedGoogle Scholar
  36. Wiberg, U. H., 1981. Sex determination in the European eel (Anguilla anguilla L.). Cytogenet. Cell Genet. 36: 589–598.Google Scholar

Copyright information

© Dr W. Junk Publishers, Dordrecht 1985

Authors and Affiliations

  • B. Mayr
    • 1
  • P. Rab
    • 3
  • M. Kalat
    • 2
  1. 1.Veterinary UniversityInstitute for Animal Breeding and GeneticsViennaAustria
  2. 2.Veterinary UniversityLudwig Boltzmann Institute for Immuno- and Cytogenetic ResearchViennaAustria
  3. 3.Institute of Animal Physiology and Genetics, Department of GeneticsCzechoslovak Academy of SciencesLibechovCzechoslovakia

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