Genetic control of rat heart allograft rejection: effect of different MHC and non-MHC incompatibilities
We investigated the genetic control of heterotopic heart allograft rejection using a family of standard inbred, major histocompatibility complex (MHC)-congenic, and intra-MHC recombinant rat strains. Gene products of the various regions within the rat MHC differed markedly in their capacity to induce rejection. Isolated incompatibility at class I antigens encoded by theRT1. A andRT1. C regions failed to induce rejection within the observation period of 100 days, whereas class II antigens encoded by theRT1.B/D region provoked rapid rejection within 10 days. By comparison of the rejection times of isolated and combined incompatibilities a number of functional interactions could be demonstrated between individual MHC regions which either prolonged or shortened allograft survival. In contrast to rapid rejection of MHC-mismatched heart allografts, differences at non-MHC histocompatibility antigens were associated with graft survival beyond 100 days, although chronic rejection of variable severity was detected histologically. Disparity at non-MHC plus class I antigens, however, provoked acute heart allograft rejection.
KeywordsMajor Histocompatibility Complex Gene Product Genetic Control Graft Survival Functional Interaction
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- Butcher, G. W. and Howard, J. C.: The MHC of the laboratory rat, Rattus norvegicus.In D. M. Weir (ed.):Handbook of Experimental Immunology, Vol. 3, pp. 101.1–101.18, Blackwell, Oxford, 1986Google Scholar
- Günther, E.: Immunogenetic aspects of organ transplantation in the rat.In A. Thiede, E. Deltz, R. Engemann, H. Hamelmann (eds.):Microsurgical Models in Rats for Transplantation Research, pp. 83–94, Springer-Verlag, Berlin, Heidelberg, 1985Google Scholar
- Guttmann, R. D., Forbes, R. D. C., Fuks, A., and Hibberd, J.: Rejection and prolongation of rat cardiac allografts across intramajor histocompatibility complex (MHC) and non-MHC differences using congenic lines: evidence for decreased class I immunogenicity.Transplant Proc 17: 1911–1913, 1985Google Scholar
- Klempnauer, J., Wonigeit, K., Steiniger, B., Günther, E., and Pichlmayr, R.: Pancreas whole organ transplantation in the rat. Differential effect of individual MHC regions.Transplant Proc 15: 1308–1310, 1983aGoogle Scholar
- Klempnauer, J., Wonigeit, K., Günther, E., and Pichlmayr, R.: Pancreas whole organ transplantation in the rat: evidence for strong effect of non-MHC incompatibilities.Transplant Proc 15: 1649–1651, 1983bGoogle Scholar
- Klempnauer, J., Wonigeit, K., and Steiniger, B.: Transplantation effects of the RT1.C region in rat heart and pancreas grafting.Transplant Proc 17: 1893–1896, 1985Google Scholar
- Mann, H. B. and Whitney, D. R.: On a test of whether one of two random variables is stochastically larger than the other.Ann Math Statist 18: 50–60, 1947Google Scholar
- Rozing, J., Bonthuis, F., Joling, P., Vaessen, L. M. B., and Lameijer, L. D. F.: The influence of RT1 subregion differences on cardiac allograft survival.Transplant Proc 15: 1647–1648, 1983Google Scholar
- Soulillou, J. P., Blandin, F., Günther, E., and Lemoine, V.: Genetics of the blood transfusion effect on heart allografts in rats.Transplantaton 38: 63–67, 1984Google Scholar
- Steiniger, B. and Klempnauer, J., and Wonigeit, K.: Phenotype and histological distribution of interstitial dendritic cells in the rat pancreas, liver, heart and kidney.Transplantation 38: 169–175, 1985aGoogle Scholar
- Steiniger, B. and Klempnauer, J., and Wonigeit, K.: Expression of class I and class II major histocompatibility complex antigens during heart allograft rejection in the rat.Transplant Proc 17: 1907–1910, 1985bGoogle Scholar
- Tsuchimoto, S., Mizuno, K., Matsuno, Y., Niiyama, T., Cramer, D., Natori, T., and Aizawa, M.: The effect of RT1 subregion differences on liver allograft survival in the rat.Transplant Proc 17: 1900–1901, 1985Google Scholar