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Immunogenetics

, Volume 30, Issue 1, pp 34–41 | Cite as

A mouse chromosome 17 gene encodes a testes-specific transcript with unusual properties

  • Nora Sarvetnick
  • Jen-Yue Tsai
  • Howard Fox
  • Stephen H. Pilder
  • Lee M. Silver
Article

Abstract

We have characterized a novel mouse gene (D17Si11) on chromosome 17 that expresses a major transcript observed uniquely in the testes. TheD17Si11 locus has been mapped to the central region of chromosome 17 betweenH-2 andC3. Sequence analysis demonstrates several unusual features of this locus and its transcript: first is the presence of complementary sets of alternating purine and pyrmidine residues within the 3′ region of the transcript that could form double-stranded, hairpin-like secondary structures with properties similar to that of Z-DNA; second is the existence of a hypothetical, long open reading frame in the nucleotide strand that is complementary to the testes transcripts. This complementary strand open reading frame is three times the size of the longest potential open reading frame present in the transcript itself. Although a function forD17Si11 has yet to be determined, the gene is relatively non-polymorphic in mice and appears conserved in mammals.

Keywords

Nucleotide Sequence Analysis Secondary Structure Central Region Purine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Nora Sarvetnick
    • 1
  • Jen-Yue Tsai
    • 2
  • Howard Fox
    • 1
  • Stephen H. Pilder
    • 2
  • Lee M. Silver
    • 2
  1. 1.Cold Spring Harbor LaboratoryCold Spring HarborUSA
  2. 2.Department of Molecular BiologyPrinceton UniversityPrincetonUSA

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