A mouse chromosome 17 gene encodes a testes-specific transcript with unusual properties
- 34 Downloads
We have characterized a novel mouse gene (D17Si11) on chromosome 17 that expresses a major transcript observed uniquely in the testes. TheD17Si11 locus has been mapped to the central region of chromosome 17 betweenH-2 andC3. Sequence analysis demonstrates several unusual features of this locus and its transcript: first is the presence of complementary sets of alternating purine and pyrmidine residues within the 3′ region of the transcript that could form double-stranded, hairpin-like secondary structures with properties similar to that of Z-DNA; second is the existence of a hypothetical, long open reading frame in the nucleotide strand that is complementary to the testes transcripts. This complementary strand open reading frame is three times the size of the longest potential open reading frame present in the transcript itself. Although a function forD17Si11 has yet to be determined, the gene is relatively non-polymorphic in mice and appears conserved in mammals.
KeywordsNucleotide Sequence Analysis Secondary Structure Central Region Purine
Unable to display preview. Download preview PDF.
- Bibbins, K. B., Tsai, J.-Y., Schimenti, J., Sarvetnick, N., Zoghbi, H. Y., Goodfellow, P., and Silver, L. M.: Human homologues of two mouse t-complex-associated loci map to unlinked regions of chromosome 6.Genomics, in press, 1989Google Scholar
- Bucan, M., Herrmann, B. G., Frischauf, A.-M., Bautch, V. L., Bode, V., Silver, L. M., Martin, G. R., and Lehrach, H.: Deletion and duplication of DNA sequences is associated with the embryonic lethal phenotype of the t9 complementation group of the mouse t complex.Genes and Development 1: 376–385, 1987PubMedGoogle Scholar
- Hammer, M., Schimenti, J., and Silver, L. M.: The evolution of mouse chromosome 17 and the origin of t complex inversions.Proc Natl Acad Sci USA, in press, 1989Google Scholar
- Klein, J.:Natural History of the Major Histocompatibility Complex, John Wiley & Sons, New York, 1986Google Scholar
- Maniatis, T., Fritsch, E. F., and Sambrook, J.:Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York 1982Google Scholar
- Nelson, D. L., Weis, J. H., Przyborski, M. J., Mulligan, R. C., Seidman, J. G., and Housman, D. E.: Metaphase chromosome transfer of introduced selectable markers.J Molec Appl Genet 2: 563–577, 1984Google Scholar
- Silver, L. M., Hammer, M., Fox, H., Garrels, J., Herrmann, B., Bucan, M., Herrmann, B., Frischauf, A.-M., Lehrach, H., Winking, H., Figueroa, F., and Klein, J.: Molecular evidence for the rapid propagation of mouse t haplotypes from a single, recent, ancestral chromosome.Mol Biol Evol 4: 473–482, 1987PubMedGoogle Scholar