Immunogenetics

, Volume 30, Issue 1, pp 5–12 | Cite as

Identification of multiple isoforms of the low-affinity human IgG Fc receptor

  • Tetsunori Seki
Article

Abstract

Two varieties of similar, but structurally distinct, cDNA clones for the human low-affinity receptors for the Fc portion of immunoglobulin G (FcγRII) have been isolated. One type of clone was obtained from human B lymphocytes, and the other from PHA-activated peripheral T cells and monocytes. Transfection of both prototype clones into Cos-7 cells and subsequent specific staining with monoclonal antibodies of the CDw32 group confirmed the identification of the gene products. The nucleotide sequence of the cDNA clone from B lymphocytes contains an open reading frame that encodes a protein of relative mass (M r) 27000 with an extracellular domain of 179 amino acids containing three potential N-glycosylation sites, a 26 amino acid transmembrane domain, and a 44 amino acid cytoplasmic domain. The clones from peripheral T cells and monocytes both encoded a protein ofM r 31000 with a 179 amino acid extracellular domain containing two potential N-glycosylation sites and a 26 amino acid transmembrane domain. The two types of clones had similar sequences in their immunoglobulin-like extracellular and transmembrane domains, but differed in their leader sequences and 3′-untranslated regions. The most notable difference between the clones was the presence of a distinctive 76 amino acid cytoplasmic domain in those isolated from T cells and monocytes.

Keywords

Nucleotide Similar Sequence cDNA Clone Untranslated Region Transmembrane Domain 

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References

  1. Anderson, C. L.: Isolation of the receptor for IgG from a human monocyte cell line (U937) and from human peripheral blood monocytes.J Exp Med 156: 1794–1806, 1982CrossRefPubMedGoogle Scholar
  2. Anderson, C. L. and Looney, R. J.: Human leukocyte IgG Fc receptors.Immunol Today 7: 264–266, 1986CrossRefGoogle Scholar
  3. Chen, C. and Okayama, H.: High efficiency transformation of mammalian cells by plasmid DNA.Mol Cell Biol 7: 2745–2752, 1987PubMedGoogle Scholar
  4. Cohen, L., Sharp, S., and Kulczycki, A., Jr.: Human monocytes, B lymphocytes, and non-B lymphocytes each have structurally unique Fcγ receptors.J Immunol 131: 378–383, 1983PubMedGoogle Scholar
  5. De Geus, P., Van Den Bergh, C. J. Kuipers, O., Verheij, H. M., Hoekstra, W. P. M., and De Haas, G. H.: Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein fromEscherichia coli.Nucleic Acids Res 15: 3743–3759, 1987PubMedGoogle Scholar
  6. Fleit, H. B., Wright, S. D., and Unkeles, J. C.: Human neutrophil Fcγ receptor distribution and structure.Proc Natl Acad Sci USA 79: 3275–3279, 1982PubMedGoogle Scholar
  7. Froese, A. and Paraskevas, F.:Structure and Function of Fc Receptors, Marcel Dekkar, New York, 1983Google Scholar
  8. Hibbs, M. L., Bonadonna, L., Scott, B. M., McKenzie, I. F. C., and Hogarth, P. M.: Molecular cloning of a human immunoglobulin G Fc receptor.Proc Acad Sci USA 85: 2240–2244, 1988aGoogle Scholar
  9. Hibbs, M. L., Classon, B. J., Walker, I. D., McKenzie, I. F. C., and Hogarth, P. M.: The structure of the murine Fc receptor for IgG.J Immunol 140: 544–550, 1988bPubMedGoogle Scholar
  10. Jones, D. H., Looney, R. J. and Anderson, C. L.: Two distinct classes of IgG Fc receptors on a human monocyte line (U937) defined by differences in binding of murine IgG subclasses at low ionic strength.J Immunol 135: 3348–3353, 1985PubMedGoogle Scholar
  11. Kozak, M.: Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAsNucleic Acids Res 12: 857–872, 1984PubMedGoogle Scholar
  12. Kulczycki, A., Jr.: Human neutrophils and eosinophils have structurally distinct Fcγ receptors.J Immunol 133: 849–854, 1984PubMedGoogle Scholar
  13. Lanier, L. L., Kipps, T. J., and Phillips, J. H.: Functional properties of a unique subset of cytotoxic CD3+ T lymphocytes that express Fc receptors for IgG (CD16/Leu-11 antigen).J. Exp Med 162: 2089–2106, 1985CrossRefPubMedGoogle Scholar
  14. Lewis, V. A., Kocj, T., Plutner, H., and Mellman, I.: A complementary DNA clone for a macrophage-lymphocyte Fc receptor.Nature 324: 372–375, 1986CrossRefPubMedGoogle Scholar
  15. Looney, R. J., Abraham, G. N., and Anderson, C. L.: Human monocytes and U937 cells bear two distinct Fc receptors for IgG.J Immunol 136: 1641–1647, 1986aPubMedGoogle Scholar
  16. Looney, R. J., Ryan, D. H., Takahashi, K., Fleit, H. B., Cohen, H. J., Abraham, G. N., and Anderson, C. L.: Identification of a second class of IgG Fc receptors on human neutrophils.J Exp Med 163: 826–836, 1986bCrossRefPubMedGoogle Scholar
  17. Maniatis, T., Fritch, E. F., and Sanbrook, J.:Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982Google Scholar
  18. Miller, J., Malek, T. R., Leonard, W. J., Greene, W. C., Shevach, E. M., and Germain, R. N.: Nucleotide sequence and expression of a mouse interleukin 2 receptor cDNA.J Immunol 134: 4212–4217, 1985PubMedGoogle Scholar
  19. Mount, S. M.: A catalogue of splice junction sequences.Nucleic Acids Res 10: 459–472, 1982PubMedGoogle Scholar
  20. Perussia, B., Starr, S., Abraham, S., Fanning, V., and Trinchieri, G.: Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions.J Immunol 130: 2133–2141, 1983PubMedGoogle Scholar
  21. Ravetch, J. V., Luster, A. D., Weinshank, R., Kochan, J., Pavloves, A., Portnoy, D. A., Hulmes, J., Pan, Y.-C. E., and Unkeles, J. C.: Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors.Science 234: 718–725, 1986PubMedGoogle Scholar
  22. Rosenfeld, S. I., Looney, R. J., Ledy, J. P., Phipps, D. C., Abraham, G. N., and Anderson, C. L.: Human platelet Fc receptor for immunoglobulin G.J Clin Invest 76: 2317–2322, 1985PubMedCrossRefGoogle Scholar
  23. Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci USA 74: 5463–5467, 1977PubMedGoogle Scholar
  24. Stengelin, S., Stamenkovic, I., and Seed, B.: Isolation of cDNAs for two distinct human Fc receptors by ligand affinity cloning.EMBO J 7: 1053–1059, 1988PubMedGoogle Scholar
  25. Stuart, S. G., Trounstine, M. L., Vaux, D. J. T., Koch, T., Martens, C. L., Mellman, I., and Moore, K. W.: Isolation and expression of cDNA clones encoding a human receptor for IgG (FcγRII)J Exp Med 166: 1668–1684, 1987CrossRefPubMedGoogle Scholar
  26. Suzuki, T., Taki, T., Hachime, K., and Sadasivan, R.: Biochemical properties of biologically active Fcγ receptors of human B lymphocytes.Mol Immunol 18: 55–65, 1981CrossRefPubMedGoogle Scholar
  27. Tetteroo, P. A. T., van der Schoot, C. E., Visser, F. J. Bos, M.-J. E., and von dem Borne, A. E. G.: Three different types of Fcγ receptors on human leucocytes defined by Workshop antibodies; FcγRlow of neutrophils FcγRlow of K/NK lymphocytes, and FcγRII.In A. J. McMichael, (ed.):Leucocyte Typing III, pp. 702–706, Springer-Verlag, New York, 1987Google Scholar
  28. Titus, J. A., Sharow, S. O., and Segal, D. M.: Analysis of Fc (IgG) receptors on human peripheral blood leukocytes by dual fluorescence flow microfluorometry.J Immunol 130: 1152–1158, 1983PubMedGoogle Scholar
  29. Unkeless, J. C., Scigliano, E. and Freedman, V. H.: Structure and function of human and murine receptors for IgG.Annu Rev Immunol 6: 251–281, 1988CrossRefPubMedGoogle Scholar
  30. Vaughn, M., Taylor, M., and Mohanakumar, T.: Characterization of human IgG Fc receptors.J. Immunol 135: 4059–4065, 1985PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Tetsunori Seki
    • 1
  1. 1.Hospital for Joint DiseasesNew York University Medical CenterNew YorkUSA

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