Immunogenetics

, Volume 30, Issue 6, pp 422–426 | Cite as

Two distinct subtypes of theHLA-DRw12 haplotypes in the Japanese population detected by nucleotide sequence analysis and oligonucleotide genotyping

  • Akio Abe
  • Ichiro Ito
  • Midori Ohkubo
  • Takehisa Kaneko
  • Kohichi Ito
  • Hidehito Kato
  • Noboru Kashiwagi
  • Fumiya Obata
Article

Abstract

We determined the DNA sequence of the enzymatically amplified second exon of theDRB1 gene of theDRw12 haplotypes derived from three Japanese donors and found two distinct subtypes of theDRw12 haplotype. The two subtypes, designatedDRw12a andDrw12b, had single-base substitutions that predicted one amino acid change at residue number 67. The sequence of theDrw12a andDRw12b subtypes differed from those of the otherDR haplotypes, but in the first hypervariable region of theDRB1 gene the sequences were identical to those of theDRw8(Dw8.1) andDRw8(Dw8.3) haplotypes. TheDRw12a andDRw12b subtypes were detected in a wide range of Japanse donors by genotyping with sequence-specific oligonucleotide probes synthesized according to the DNA sequence of the two subtypes. Results of this study demonstrated that theDRw12 haplotypes in the Japanese population are genetically diverse, as many otherDR haplotypes are.

Keywords

Nucleotide Sequence Analysis Nucleotide Sequence Amino Acid Change Japanese Population 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Akio Abe
    • 1
  • Ichiro Ito
    • 1
  • Midori Ohkubo
    • 1
  • Takehisa Kaneko
    • 1
  • Kohichi Ito
    • 1
  • Hidehito Kato
    • 1
  • Noboru Kashiwagi
    • 1
  • Fumiya Obata
    • 1
  1. 1.Department of Transplantation ImmunologyKitasato University School of MedicineSagamiharaJapan

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