Abstract
Monoclonal antibodies (mAbs) W6/32, HC10, and 4E were used to precipitate class I antigens from 21 selected individuals with at least oneHLA-C “blank” allele. In 19 of these individuals, characteristicHLA-C banding patterns which could be precipitated by all three HLA class I mAbs were observed on one-dimensional isoelectric focusing gels-obviously the gene products ofHLA-C “blank”. At least four allelic HLA-C “blank” gene products with different isoelectric points could be discerned. All of them segregated withHLA-C “blank” haplotypes in informative families; two of them were associated withHLA-B51, one withHLA-B38, and one withHLA-B18. Reactivity of the HLA-C “blank” heavy chains with mAb W6/32 indicates that they are able to associated with beta-2 microglobulin, and hence are most probably expressed at the cell surface.
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Hajek-Rosenmayr, A., Jungl, L., Stammler, M. et al. HLA-C “blank” alleles express class I gene products. Biochemical analysis of four different HLA-C “blank” polypeptides. Immunogenetics 30, 399–404 (1989). https://doi.org/10.1007/BF02421170
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DOI: https://doi.org/10.1007/BF02421170