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Plant Molecular Biology

, Volume 4, Issue 5, pp 267–273 | Cite as

Nuclear and mitochondrial DNA have sequence homology with a chloroplast gene

  • Dara L. Whisson
  • N. Steele Scott
Article

Summary

A PstI 7.7 kbp fragment from chloroplast (ct) DNA of spinach shows homology to an EcoRI 8.3 kbp fragment of mitochondrial (mt) DNA and in turn, both are homologous to a number of common regions of nuclear (n) DNA. The common area of homology between the chloroplast and mitochondrial fragments is between a KpnI 1.8 segment internal to the PstI sites in the ctDNA and an EcoRI/BamHI 2.9 kbp fragment at one end of the mitochondrial 8.3 kbp fragment. The KpnI 1.8 kbp ctDNA fragment is within a structural gene for the P700 chlorophyll a apoprotein. Further analysis of this KpnI 1.8 kbp fragment confined the homologous region in mtDNA to a ct 0.8 kbp HpaII fragment. These smaller pieces of the organellar genomes share homologies with nuclear DNA as well as displaying unique hybridization sites. The observations reported here demonstrate that there is a common or closely related sequence in all three genetic compartments of the cell.

Keywords

chloroplast DNA DNA homologies mitochondrial DNA nuclear DNA photosystem I spinach 

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References

  1. 1.
    Alt J, Westhoff P, Sears BB, Nelson N, Hurt E, Hauska G, Herrmann RG: Genes and transcripts for the polypeptides of the cytochrome b6/f complex from spinach thylakoid membranes. EMBO J 2:979–986, 1983.PubMedGoogle Scholar
  2. 2.
    Alt J, Winter P, Sebald W, Moser JG, Schedel R, Westhoff P, Herrmann RG: Localization and nucleotide sequence of the gene for the ATP synthase proteolipid subunit on the spinach plastid chromosome. Curr Genet 7:129–138, 1983.CrossRefGoogle Scholar
  3. 3.
    Doolittle WF: Revolutionary concepts in evolutionary cell biology. Trends Biochem Sci 5:146–149, 1980.CrossRefGoogle Scholar
  4. 4.
    Douce R, Moore AL, Neuberger M: Isolation and oxidation properties of intact mitochondria isolated from spinach leaves. Plant Physiol 60:625–628, 1977.PubMedCrossRefGoogle Scholar
  5. 5.
    Dretzen G, Bellard M, Sassone-Corsi P, Chambon P: A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem 112:295–298, 1981.CrossRefPubMedGoogle Scholar
  6. 6.
    Driesel AJ, Speirs J, Bohnert HJ: Spinach chloroplast mRNA for a 32 000 Dalton polypeptide: Size and location on the physical map of the chloroplast DNA. Biochim Biophys Acta 610:297–310, 1980.PubMedGoogle Scholar
  7. 7.
    Dugaiczyk A, Boyer HW, Goodman HM: Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol 96:171–184, 1975.CrossRefPubMedGoogle Scholar
  8. 8.
    Ellis RJ: Chloroplast proteins: synthesis, transport and assembly. Ann Rev Plant Physiol 32:111–137, 1981.CrossRefGoogle Scholar
  9. 9.
    Ellis J: Mobile genes of chloroplasts and the promiscuity of DNA. Nature 304:308–309, 1983.CrossRefGoogle Scholar
  10. 10.
    Farrelly F, Butow RA: Rearranged mitochondrial genes in the yeast nuclear genome. Nature 301:296–301, 1983.CrossRefPubMedGoogle Scholar
  11. 11.
    Grunstein M, Hogness D: Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci 72:3961–3965, 1975.PubMedGoogle Scholar
  12. 12.
    Holmes DS, Quigley M: A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197, 1981.CrossRefPubMedGoogle Scholar
  13. 13.
    Kemble RJ, Mans RJ, Gabay-Laughnan S, Loughnan JR: Sequences homologous to episomal mitochondrial DNAs in the maize nuclear genome. Nature 304:744–747, 1983.CrossRefGoogle Scholar
  14. 14.
    Lonsdale DM, Hodge TP, Howe CJ, Stern DB: Maize mitochondrial DNA contains a sequence homologous to the ribulose-1,5-bisphosphate carboxylase large subunit gene of chloroplast DNA. Cell 34:1007–1014, 1983.CrossRefPubMedGoogle Scholar
  15. 15.
    Palmer JD, Shields CR: Tripartite structure of theBrassica compestris mitochondrial genome. Nature 307:437–440, 1984.CrossRefGoogle Scholar
  16. 16.
    Palmer JD, Thompson WF: Clone banks of the mung bean, pea and spinach chloroplast genomes. Gene 15:21–26, 1981.CrossRefPubMedGoogle Scholar
  17. 17.
    Possingham JV, Smith JW: Factors affecting chloroplast replication in spinach. J Exp Bot 23:1050–1059, 1972.Google Scholar
  18. 18.
    Scott NS, Possingham JV: Chloroplast DNA in expanding spinach leaves. J Exp Bot 123:1081–1092, 1980.Google Scholar
  19. 19.
    Scott NS, Timmis JN: Homologies between nuclear and plastid DNA in spinach. Theor Appl Genet 67:279–288, 1984.CrossRefGoogle Scholar
  20. 20.
    Stern DB, Lonsdale DM: Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature 299:698–702, 1982.CrossRefPubMedGoogle Scholar
  21. 21.
    Stern DB, Palmer JD: Extensive and widespread homologies between mitochondrial DNA and chloroplast DNA in plants. Proc Natl Acad Sci USA, in press (1984).Google Scholar
  22. 22.
    Timmis JN, Scott NS: Sequence homology between spinach nuclear and chloroplast genomes. Nature 305:65–67, 1983.CrossRefGoogle Scholar
  23. 23.
    Timmis JN, Scott NS: Promiscuous DNA: Sequence homologies between DNA of separate organelles. TIBS 9(6):271–273, 1984.Google Scholar
  24. 24.
    van den Bogaart P, Samallo J, Agsteribbe E: Similar genes for a mitochondrial ATPase subunit in nuclear and mitochondrial genomes ofNeurospora crassa. Nature 298:187–189, 1982.CrossRefGoogle Scholar
  25. 25.
    Westhoff P, Alt J, Nelson N, Bottomley W, Bunemann H, Herrman RG: Genes and transcripts for the P700 chlorophyll a apoprotein and subunit 2 of the photosystem I reaction center complex from spinach thylakoid membranes. Pl Mol Biol 2:95–107, 1983.CrossRefGoogle Scholar
  26. 26.
    Westhoff P, Nelson N, Bunemann H, Herrmann RG: Localization of genes for coupling factor subunits on the spinach plastid chromosome. Curr Genet 4:109–120, 1981.CrossRefGoogle Scholar
  27. 27.
    Whitfeld PR, Bottomley W: Mapping of the gene for the large subunit of ribulose bisphosphate carboxylase on spinach chloroplast DNA. Biochem Int 1:172–178, 1980.Google Scholar
  28. 28.
    Wright RM, Cummings DJ: Integration of mitochondrial gene sequences within the nuclear genome during senescence in a fungus. Nature 302:86–88, 1983.CrossRefPubMedGoogle Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1985

Authors and Affiliations

  • Dara L. Whisson
    • 1
  • N. Steele Scott
    • 1
  1. 1.CSIRO Division of Horticultural ResearchAdelaideSouth Australia

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