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Characterization of developing oat seed mRNA: evidence for many globulin mRNAs

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Abstract

Polyadenylated mRNA from developing oat (Avena sativa L.) seeds was isolated and analyzed. Prominent mRNA species of 18S, 15S and 12S were observed; the 18S mRNA was judged to be esentially free of ribosomal RNA by hybridization analysis. Size fractionation andin vitro translation of this mRNA was performed. SDS, IEF-SDS gel electrophoresis and immunoprecipitation were used to analyze the translation products. It is shown that globulin mRNA (18S) accounts for roughly 30% of the total mRNA in developing seeds, the 12S and 15S mRNAs accounting for the remainder. The 18S mRNA directs the synthesis of a series of distinct but related polypeptides, suggesting that some of the heterogeneity seen in the oat globulins is at the amino acid sequence level.

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Abbreviations

IEF:

isoelectric focusing

SDS:

sodium dodecylsulfate

KD:

Kilo or thousands of daltons, 2′, 3′ AMP, 2′, 3′ Adenosine monophosphate, mixed 2′ and 3′ isomers

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Author notes

  1. Gregory J. Matlashewski

    Present address: Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, WC2A 3PX, London, U.K.

Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, University of Ottawa, K1N9B4, Ottawa, Ontario, Canada

    Steven Fabijanski, Gregory J. Matlashewski & Illimar Altosaar

Authors
  1. Steven Fabijanski
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  2. Gregory J. Matlashewski
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  3. Illimar Altosaar
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Fabijanski, S., Matlashewski, G.J. & Altosaar, I. Characterization of developing oat seed mRNA: evidence for many globulin mRNAs. Plant Mol Biol 4, 205–210 (1985). https://doi.org/10.1007/BF02418237

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  • DOI: https://doi.org/10.1007/BF02418237

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