Abstract
A brief pulse of red light (R) given to darkgrown seedlings ofArabidopsis thaliana (L.) Heyn. potentiates rapid synthesis of chlorophyll upon transfer to continuous white light. The time course for potentiation of rapid greening shows that a R pulse in the LF (low fluence) range has maximal effect within a few hours, and that there is a small VLF (very low fluence) component as well. Partial reversal of the effect of R by far-red light (FR) indicates that the pulse acts through phytochrome. As it does in the wild-type (WT), a pulse of R accelerates greening of long-hypocotyl (hy) mutants. The extent of induction by the R pulse was about the same in the WT and in allhy mutants studied. Reversibility by FR was greatly decreased in thehy-1 andhy-2 strains. It is possible that these mutants contain a species of phytochrome with defective phototransformation kinetics. If there is such a defective phytochrome species, it nevertheless appears to be active in the potentiation of rapid greening.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- R:
-
red light
- FR:
-
far-red light
- DMF:
-
N,N-dimethylformamide
- LF:
-
low-fluence phytochrome response
- VLF:
-
very-low-fluence response
- WT:
-
wild-type
- LHC(II):
-
light harvesting chlorophyll a/b binding proteins of photosystem II
References
Adamse, P., Kendrick, R.E., Koornneef, M. (1988) Yearly review: photomorphogenetic mutants of higher plants. Photochem. Photobiol.48, 833–841
Briggs, W.R., Mösinger, E., Schäfer, E. (1988) Phytochrome regulation of greening in barley—effects on chlorophyll accumulation. Plant Physiol.86, 435–440
Chory, J., Peto, C.A., Ashbaugh, M., Saganich, R., Pratt, L.H., Ausubel, F. (1989) Different roles for phytochrome in etiolated and green plants deduced from characterization ofArabidopsis thaliana mutants. Plant Cell1, 867–880
Cone, J.W., Kendrick, R.E. (1985) Fluence-response curves and action spectra for promotion and inhibition of seed germination in wildtype and long-hypocotyl mutants ofArabidopsis thaliana L. Planta163, 43–54
Ellis, R.J. (1986) Photoregulation of plant gene expression. Biosci. Rep.6, 127–136
Estelle, M.A., Somerville, C.R. (1986) The mutants ofArabidopsis. Trends Genet.2, 89–93
Fluhr, R., Kuhlemeier, C., Nagy, F., Chua, N.H. (1986) Organspecific and light-induced expression of plant genes. Science232, 1106–1112
Giuliano, G., Pichersky, E., Malik, V.S., Timko, M.P., Scolnik, P.A., Cashmore, A.R. (1988) An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc. Natl. Acad. Sci. USA85, 7089–7093
harpster, M., Apel, K. (1985) The light-dependent regulation of gene expression during plastid development in higher plants. Physiol. Plant.64, 147–152
Horwitz, B.A., Thompson, W.F., Briggs, W.R. (1988) Phytochrome regulation of greening inPisum: chlorophyll accumulation and abundance of mRNA for the light-harvesting chlorophyll a/b binding proteins. Plant Physiol.86, 299–305
Karlin-Neumann, G.A., Sun, L., Tobin, E.M. (1988) Expression of light-harvesting chlorophyll a/b-protein genes in phytochrome-regulated in etiolatedArabidopsis thaliana seedlings. Plant Physiol.88, 1323–1331
Kasemir, H., Oberdorfer, U., Mohr, H. (1973) A twofold action of phytochrome in controlling chlorophyll a accumulation. Photochem. Photobiol.18, 481–486
Kaufman, L.S., Thompson, W.F., Briggs, W.R. (1984) Different red light requirements for phytochrome-induced accumulation of cab RNA and rbcS RNA. Science226, 1447–1449
Ken-Dror, S., Horwitz, B.A. (1990) Altered phytochrome regulation of greening in anaurea mutant of tomato. Plant Physiol. (in press)
Koornneef, M., Rolff, E., Spruit, C.J.P. (1980) Genetic control of light-inhibited hypocotyl elongation inArabidopsis thaliana (L.) Heynh. Z. Pflanzenphysiol.100, 147–160
Koornneef, M., Cone, J.W., Dekens, R.G., O'Herne-Robers, E.G., Spruit, C.J.P., Kendrick, R.E. (1985) Photomorphogenic responses of long hypocotyl mutants of tomato. J. Plant Physiol.120, 153–165
Lam, E., Green, P.J., Wong, M., Chua, N-H. (1989) Phytochrome activation of two nuclear genes requires cytoplasmic protein synthesis. EMBO J.8, 2777–2783
Mohr, H. (1972) Lectures on photomorphogenesis. Springer, Berlin Heidelberg New York
Moran, R. (1982) Formulae for determination of chlorophyllous pigments extracted with N,N-dimethylformamide. Plant Physiol.69, 1376–1381
Mösinger, E., Batschauer, A., Apel, K., Schäfer, E., Briggs, W.R. (1988) Phytochrome regulation of greening in barley. Effects on mRNA abundance and on transcriptional activity of isolated nuclei. Plant Physiol.86, 706–710
Nagy, F., Kay, S.A., Chua, N.H. (1988) Gene regulation by phytochrome. Trends Genet.4, 37–42
Oelmüller, R., Kendrick, R.E., Briggs, W.R. (1989) Blue-light mediated accumulation of nuclear-encoded transcripts coding for proteins of the thylakoid membrane is absent in the labile phytochrome-deficientaurea mutant of tomato. Plant Mol. Biol.13, 223–232
Parks, B.M., Jones, A.M., Adamse, P., Koornneef, M., Kendrick, R.E., Quail, P.H. (1987) Theaurea mutant of tomato is deficient in spectrophotometrically and immunochemically detectable phytochrome. Plant Mol. Biol.9, 97–107
Parks, B.M., Shanklin, J., Koornneef, M., Kendrick, R.E., Quail, P.H. (1989) Immunochemically detectable phytochrome is present at normal levels but is photochemically nonfunctional in thehy 1 andhy 2 long hypocotyl mutants ofArabidopsis. Plant Mol. Biol.12, 425–437
Raven, C.W., Shropshire, W., Jr. (1975) Photoregulation of logarithmic fluence-response curves for phytochrome control of chlorophyll formation inPisum sativum L. Photochem. Photobiol.21, 423–429
Sharrock, R.A., Parks, B.M., Koornneef, M., Quail, P.H. (1988) Molecular analysis of the phytochrome deficiency in anaurea mutant of tomato. Mol. Gen. Genet.213, 9–14
Virgin, H.I. (1972) Chlorophyll biosynthesis and phytochrome action. In: Phytochrome, pp. 371–404, Mitrakos, K., Shropshire, W., Jr., eds. Academic Press, New York
Author information
Authors and Affiliations
Additional information
Dedicated to Professor Hans Mohr on the occasion of his 60th birthday
Rights and permissions
About this article
Cite this article
Lifschitz, S., Gepstein, S. & Horwitz, B.A. Phytochrome regulation of greening in wild type and long-hypocotyl mutants ofArabidopsis thaliana . Planta 181, 234–238 (1990). https://doi.org/10.1007/BF02411544
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02411544