Planta

, Volume 181, Issue 2, pp 229–233 | Cite as

Prenyl lipid and fatty-acid synthesis in isolatedAcetabularia chloroplasts

  • Ralf Bäuerle
  • Friedhelm Lütke-Brinkhaus
  • Bodo Ortmann
  • Sigrid Berger
  • Hans Kleinig
Article

Abstract

A gentle procedure allowed the isolation of intact and highly active chloroplasts from the unicellular green algaAcetabularia mediterranea. These chloroplasts incorporated carbon from NaH14CO3 into fatty acids and prenyl lipids at a rate of about 20–50 nmol carbon· (mg chlorophyll)−1·h−1. Most of the fatty acids formed in vitro were esterified in galactolipids. The main prenyl lipids synthesized were the chlorophyll side chain, intermediates of the carotenogenic path, α-and β-carotene, as well as lutein. Large amounts of [1-14C]acetate were incorporated, but exclusively into fatty acids.Isopentenyl diphosphate was a good substrate for prenyl-lipid formation in hypotonically treated chloroplasts. The envelope of intact chloroplasts, however, was impermeable to this compound. Intermediates of the mevalonate pathway were not accepted as precursors under conditions whereisopentenyl diphosphate was well incorporated. The results show that the lipid biosynthetic pathways in the plastids ofAcetabularia, a member of the ancient family of Dasycladaceae, are very similar to those in higher-plant plastids.

Key words

Acetabularia Acyl lipid formation Chloroplast (fatty-acid synthesis) Fatty acid (synthesis) Prenyl lipid formation Mevalonate pathway 

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References

  1. Andrews, J., Schmidt, H., Heinz, E. (1989) Interference of electron transport inhibitors with desaturation of monogalactosyl diacylglycerol in intact chloroplasts. Arch. Biochem. Biophys.270, 158–165CrossRefGoogle Scholar
  2. Allen, B.E., Banthorpe, D.V. (1981) Partial purification and properties of prenyltransferase fromPisum sativum. Phytochemistry20, 35–40CrossRefGoogle Scholar
  3. Bergmeyer, H.U. (1983) Methods of Enzymatic Analysis, vol. II, pp. 165–166, Verlag Chemie, WeinheimGoogle Scholar
  4. Beyer, P., Kreuz, K., Kleinig, H. (1980) β-Carotene synthesis in isolated chromoplasts fromNarcissus pseudonarcissus. Planta150, 435–438CrossRefGoogle Scholar
  5. Beyer, P., Weiss, G., Kleinig, H. (1985) Solubilization and reconstitution of the membrane-bound carotenogenic enzymes from daffodil chromoplasts. Eur. J. Biochem.153, 341–346CrossRefPubMedGoogle Scholar
  6. Beyer, P., Mayer, M., Kleinig, H. (1989) Molecular oxygen and the state of geometric isomerism of intermediates are essential in the carotene desaturation and cyclization reactions in daffodil chromoplasts. Eur. J. Biochem.184, 141–150CrossRefPubMedGoogle Scholar
  7. Bligh, E.D., Dyer, W.J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol.37, 911–917PubMedGoogle Scholar
  8. Daleo, G.R., Pont Lezica, R. (1977) Synthesis of dolichol phosphate by a cell-free extract from pea. FEBS Lett.74, 247–250CrossRefPubMedGoogle Scholar
  9. Frosch, S., Jabben, M., Bergfeld, R., Kleinig, H., Mohr, H. (1979) inhibition of carotenoid biogenesis by the herbicide SAN 9789 and its consequences for the action of phytochrome on plastidogenesis. Planta145, 497–505CrossRefGoogle Scholar
  10. Gray, J.C. (1987) Control of isoprenoid biosynthesis in higher plants. Adv. Bot. Res.14, 25–91CrossRefGoogle Scholar
  11. Kamptner, E. (1958) Über das System und die Stammesgeschichte der Dasycladaceen (Siphoneae verticillatae). Ann. Naturhist. Mus. Wien62, 95–122Google Scholar
  12. Kleinig, H. (1989) The role of plastids in isoprenoid biosynthesis. Annu. Rev. Plant Physiol. Plant Mol. Biol.40, 39–59CrossRefGoogle Scholar
  13. Liedvogel, B. (1985) A new radiochemical method for determination of pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase. Anal. Biochem.148, 182–189CrossRefPubMedGoogle Scholar
  14. Liedvogel, B. (1986) Acetyl coenzyme A and isopentenylpyrophosphate as lipid precursors in plant cells — Biosynthesis and compartmentation. J. Plant Physiol.124, 211–222Google Scholar
  15. Liedvogel, B., Kleinig, H. (1980) Phosphate translocator and adenylate translocator in chromoplast membranes. Planta150, 170–173CrossRefGoogle Scholar
  16. Lilley, R.M.C., Fitzgerald, M.P., Rienist, K.G., Walker, D.A. (1975) Criteria of intactness and the photosynthetic activity of spinach chloroplast preparations. New Phytol.75, 1–10CrossRefGoogle Scholar
  17. Lord, J.M., Kagawa, T., Moore, T.S., Beevers, H. (1973) Endoplasmic reticulum as the site of lecithin formation in castor bean endosperm. J. Cell. Biol.57, 659–667CrossRefPubMedGoogle Scholar
  18. Moore, F.D., Shephard, D.C. (1977) Biosynthesis in isolatedAcetabularia chloroplasts. II. Plastic pigments. Protoplasma92, 167–175CrossRefPubMedGoogle Scholar
  19. Narita, J.O., Gruissem, W. (1989) Tomato hydroxymethylglutaryl-CoA reductase is required early in fruit development but not during ripening. Plant Cell1, 181–190CrossRefPubMedGoogle Scholar
  20. Roughan, P.G., Holland, R., Slack, C.R. (1978) Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spinach chloroplasts. Biochem. J.184, 565–569Google Scholar
  21. Schulze-Siebert, D., Schultz, G. (1987) β-Carotene synthesis in isolated spinach chloroplasts. Plant Physiol.84, 1233–1237PubMedCrossRefGoogle Scholar
  22. Schweiger, H.-G., Dehm, P., Berger, S. (1972) Culture conditions forAcetabularia. In: Progress inAcetabularia research, pp. 319–330, Woodcock, C.L.F., ed. Academic Press, New YorkGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Ralf Bäuerle
    • 1
  • Friedhelm Lütke-Brinkhaus
    • 1
  • Bodo Ortmann
    • 1
  • Sigrid Berger
    • 2
  • Hans Kleinig
    • 1
  1. 1.Institut für Biologie II, ZellbiologieUniversität FreiburgFreiburg
  2. 2.Max-Planck-Institut für ZellbiologieLadenburgGermany

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