Abstract
Our aim was to identify, using their complementary DNAs (cDNAs), genes which are rapidly and transiently expressed during the initial phase of blue-light-induced chloroplast differentiation in cultured plant cells (Chenopodium rubrum L.), and to evaluate the role of their prospective products with regard to light perception, signal transduction and response, as well as coordination in the expression of other blue-light-induced genes. A cDNA library (λ gt10) was established using polyadenylated RNAs from cells exposed to blue light for 6, 12, and 24 h. Selection of the relevant species from the combined preparations was achieved by applying hybridization techniques and hydroxylapatite chromatography, thus eliminating most of the mRNAs representative of dark-grown and fully greened cells. By differential screening, several clones corresponding to genes rapidly induced by blue light were identified. For most of these a temporary accumulation of the specific mRNA between 30 min and 72 h of blue-light irradiation was observed. With regard to the nucleotide sequence and the respective deduced amino-acid sequence, a glycine-rich protein, a β-tubulin-like protein and one species resembling an acidic ribosomal protein (RLAO) were among the products of the early light-induced genes.
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Abbreviations
- cDNA:
-
complementary DNA
- kDa:
-
kilodaltons
- poly(A)RNA:
-
polyadenylated RNA
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Dedicated to Professor Hans Mohr on the occasion of his 60th birthday
Sequence data reported will appear in the EMBL Genbank and DDBJ Nucleotide Sequence Databases under the following accession numbers: X14067 for clone CRHC1 (glycine-rich protein), X15456 for clone CRHG1 (tubulin-like protein) and X15206 for clone CRE2 (RLAO-like protein)
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Kaldenhoff, R., Richter, G. Light induction of genes preceding chloroplast differentiation in cultured plant cells. Planta 181, 220–228 (1990). https://doi.org/10.1007/BF02411542
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DOI: https://doi.org/10.1007/BF02411542