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Planta

, 180:96 | Cite as

The role of high-mannose and complex asparagine-linked glycans in the secretion and stability of glycoproteins

  • Azeddine Driouich
  • Pascale Gonnet
  • Mouna Makkie
  • Anne-Catherine Laine
  • Loïc Faye
Article

Abstract

Suspension-cultured cells of sycamore (Acer pseudoplatanus L.) secrete a number of acid hydrolases and other proteins that have both highmannose and complex asparagine-linked glycans. We used affinity chromatography with concanavalin A and an antiserum specific for complex glycans in conjunction with in vivo-labeling studies to show that all of the secreted proteins carry glycans. The presence of complex glycans on secretory proteins indicates that they are passing through the Golgi complex on the way to the extracellular compartment. The sodium ionophore, monensin, did not block the transport of proteins to the extracellular medium, even though monensin efficiently inhibited the Golgi-mediated processing of complex glycans. The inhibition of N-glycosylation by tunicamycin reduced by 76% to 84% the accumulation of newly synthesized (i.e. radioactively labeled) protein that was secreted by the sycamore cells, while cytoplasmic protein biosynthesis was not affected by this antibiotic. However, in the presence of glycoprotein-processing inhibitors, such as castanospermine and deoxymannojirimycin, the formation of complex glycans was prevented but glycoprotein secretion was unchanged. These results support the conclusion that N-linked glycan processing is not necessary for sorting, but glycosylation is required for accumulation of secreted proteins in the extracellular compartment.

Key words

Acer (glycoprotein) Glycoprotein Secretion (glycoprotein) 

Abbreviations

ConA

concanavalin A

ER

endoplasmic reticulum

Mr

relative molecular mass

SDS-PAGE

sodium dodecylsulfate-polyacrylamide gel electrophoresis

GlcN

glucosamine

GlcNAc

N-acetylglucosamine

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Azeddine Driouich
    • 1
  • Pascale Gonnet
    • 1
  • Mouna Makkie
    • 1
  • Anne-Catherine Laine
    • 1
  • Loïc Faye
    • 1
  1. 1.C.N.R.S.-UA 203Université de Rouen, Faculté des SciencesMont Saint AignanFrance

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