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Quantitative correlation of peripheral and intrinsic core polypeptides of photosystem II with photosynthetic electron-transport activity ofAcetabularia mediterranea in red and blue light

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Abstract

The high photosynthetic activity (O2 production and CO2 consumption) ofAcetabularia mediterranea Lamour. (=A. acetabulum (L.) Silva) characteristic of cells cultured in white light decreases slowly when cells are kept in continuous red light, and is less than 20% of the original activity after three weeks. Subsequent blue irradiation restores the original activity completely within 3–5 d. The polypeptide composition of the thylakoids from cells grown in either red or blue light and after transfer from red to blue light was analyzed mainly with regards to photosystem II (PSII). The P700-containing reaction-centre complex of photosystem I, CPI, showed only minor quantitative alterations as a consequence of the growth-light quality, which correlated well with the activity of photosystem I under these conditions. In PSII, no drastic changes occurred in the quantity of the reaction-centre components D1 (herbicide-binding polypeptide) and D2, as determined by immunoblots. Likewise, the proteins associated with the water-splitting apparatus did not change detectably in thylakoids from red- or blue-light-treated cells (the 16-kDa component could not be found inAcetabularia thylakoids). The level of the major light-harvesting complex was completely unaffected by the light quality. In contrast, the quantities of the chlorophyll a-protein complexes of the core antenna, CP43 and CP47 (and probably CP29), changed, with kinetics similar to those of total photosynthetic activity. We postulate that the function of the PSII antenna became increasingly impaired in the absence of blue light (i.e. in red light), while blue light had a restoring effect. The peripheral antenna, comprising the light-harvesting complexes, is probably functionally connected with the reaction-centre chlorophylls via the core antenna chlorophyll-protein complexes (CP43, CP47 and probably CP29). A deficiency of these complexes would lead to uncoupling of antenna and reaction centre in the majority of PSII complexes after long periods of red-light treatment.

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Abbreviations

Chl:

chlorophyll

D1:

32-kDa QB-binding reaction-centre subunit of photosystem II

D2:

34-kDa QA-containing subunit of the reaction centre of photosystem II

LHCP:

lightharvesting chlorophyll-protein complex of photosystem II

LiDS:

lithium dodecylsulfate

Mr :

relative molecular mass

PSI, PSII:

photosystem I, II

RuBPCase:

ribulose-1,5-bisphosphate carboxylase

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Authors and Affiliations

  1. Institut für Pflanzenphysiologie, Zellbiologie und Mikrobiologie, Freie Universität Berlin, Königin-Luise-Straße 12-16, 1000, Berlin

    Rainer Schmid, Rainer Wennicke & Sigrun Fleischhauer

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  1. Rainer Schmid
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  2. Rainer Wennicke
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Schmid, R., Wennicke, R. & Fleischhauer, S. Quantitative correlation of peripheral and intrinsic core polypeptides of photosystem II with photosynthetic electron-transport activity ofAcetabularia mediterranea in red and blue light. Planta 182, 391–398 (1990). https://doi.org/10.1007/BF02411390

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  • DOI: https://doi.org/10.1007/BF02411390

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