, Volume 182, Issue 3, pp 363–369 | Cite as

Stabilization of cortical microtubules by the cell wall in cultured tobacco cells

Effects of extensin on the cold-stability of cortical microtubules
  • Tomohiro Akashi
  • Shinji Kawasaki
  • Hiroh Shibaoka


Cortical microtubules (MTs) in protoplasts prepared from tobacco (Nicotiana tabacum L.) BY-2 cells were found to be sensitive to cold. However, as the protoplasts regenerated cell walls they became resistant to cold, indicating that the cell wall stabilizes cortical MTs against the effects of cold. Since poly-l-lysine was found to stabilize MTs in protoplasts, we examined extensin, an important polycationic component of the cell wall, and found it also to be effective in stabilizing the MTs of protoplasts. Both extensin isolated from culture filtrates of tobacco BY-2 cells and extensin isolated in a similar way from cultures of tobacco XD-6S cells rendered the cortical MTs in protoplasts resistant to cold. Extensin at 0.1 mg·ml−1 was as effective as the cell wall in this respect. It is probable that extensin in the cell wall plays an important role in stabilizing cortical MTs in tobacco BY-2 cells.

Key words

Cell culture (MT stability) Cell wall and MT stability Extensin Microtubule (stability) Nicotiana (MT stabilization) Protoplast (microtubules) Temperature and MT stability 



2,4-dichlorophenoxyacetic acid

KP buffer

potassium phosphate buffer




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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Tomohiro Akashi
    • 1
  • Shinji Kawasaki
    • 2
  • Hiroh Shibaoka
    • 1
  1. 1.Department of Biology, Faculty of ScienceOsaka UniversityOsakaJapan
  2. 2.National Institute of Agrobiological ResourcesTsukuba Science City, IbarakiJapan

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