Abstract
Cortical microtubules (MTs) in protoplasts prepared from tobacco (Nicotiana tabacum L.) BY-2 cells were found to be sensitive to cold. However, as the protoplasts regenerated cell walls they became resistant to cold, indicating that the cell wall stabilizes cortical MTs against the effects of cold. Since poly-l-lysine was found to stabilize MTs in protoplasts, we examined extensin, an important polycationic component of the cell wall, and found it also to be effective in stabilizing the MTs of protoplasts. Both extensin isolated from culture filtrates of tobacco BY-2 cells and extensin isolated in a similar way from cultures of tobacco XD-6S cells rendered the cortical MTs in protoplasts resistant to cold. Extensin at 0.1 mg·ml−1 was as effective as the cell wall in this respect. It is probable that extensin in the cell wall plays an important role in stabilizing cortical MTs in tobacco BY-2 cells.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- KP buffer:
-
potassium phosphate buffer
- MT(s):
-
microtubule(s)
References
Akashi, T., Shibaoka, H. (1987) Effects of gibberellin on the arrangement and the cold stability of cortical microtubules in epidermal cells of pea internodes. Plant Cell Physiol.28, 339–348
Akashi, T., Izumi, K., Nagano, E., Enomoto, M., Mizuno, K., Shibaoka, H. (1988) Effects of propyzamide on tobacco cell microtubules in vivo and in vitro. Plant Cell Physiol.29, 1053–1062
Altman, A., Kaur-Sawhney, R., Galston, A.W. (1977) Stabilization of oat leaf protoplasts through polyamine-mediated inhibition of senescence. Plant Physiol.60, 570–574
Dustin, P. (1984) Microtubules. Springer, Berlin Heidelberg New York
Gunning, B.E.S. (1982) The cytokinetic apparatus: its development and spatial regulation. In: The cytoskeleton in plant growth and development, pp. 229–292, Lloyd, C.W., ed. Academic Press, London New York
Hardham, A.R., Gunning, B.E.S. (1978) Structure of cortical microtubule arrays in plant cells. J Cell Biol.77 14–34
Hasezawa, S., Syōno, K. (1983) Hormonal control of elongation of tobacco cells derived from protoplasts. Plant Cell Physiol.24, 127–132
Juniper, B.E., Lawton, J.R. (1979) The effect of caffeine, different fixation regimes and low temperature on microtubules in the cells of higher plants. Planta145, 411–416
Kakimoto, T., Shibaoka, H. (1986) Calcium-sensitivity of cortical microtubules in the green algaMougeotia. Plant Cell Physiol.27, 91–101
Kato, K., Matsumoto, T., Koiwai, A., Mizusaki, S., Nishida, K., Noguchi, M., Tamaki, E. (1972) Liquid suspension culture of tobacco cells. In: Fermentation technology today, pp. 689–695, Terui, G., ed. Society of Fermentation Technology of Japan, Osaka
Katsuta, J., Shibaoka, H. (1988) The roles of the cytoskeleton and the cell wall in nuclear positioning in tobacco BY-2 cells. Plant Cell Physiol.29, 403–413
Kaur-Sawhney, R., Flores, H.E., Galston, A.W. (1981) Polyamine oxidase in oat leaves: a cell wall-localized enzyme. Plant Physiol.68, 494–498
Kawasaki, S. (1989) Extensin secreted into the culture medium by tobacco cells. I. Purification and some properties. Plant Cell Physiol.30, 259–265
Kobayashi, H., Fukuda, H., Shibaoka, H. (1988) Interrelation between the spatial disposition of actin filaments and microtubules during the differentiation of tracheary elements in culturedZinnia cells. Protoplasma143, 29–37
Kropf, D.L., Kloareg, B., Quatrano, R.S. (1988) Cell wall is required for fixation of the embryonic axis inFucus zygotes. Science239, 187–190
Lamport, D.T.A., Catt, J.W. (1981) Glycoproteins and enzymes of the cell wall. In: Encyclopedia of Plant Physiology, N.S. vol. 13B; Plant carbohydrates II, pp. 133–165, Tanner, W., Loewus, F.A., eds. Springer, Berlin Heidelberg New York
Lloyd, C.W., Traas, J.A. (1988) The role of F-actin in determining the division plane of carrot suspension cells. Drug studies. Development102, 211–221
Marchant, H.J. (1978) Microtubules associated with the plasma membrane isolated from protoplasts of the green algaMougeotia. Exp. Cell Res.115, 25–30
Marchant, H.J., Hines, E.R. (1979) The role of microtubules and cell-wall deposition in elongation of regenerating protoplasts ofMougeotia. Planta146, 41–48
Mita, T., Shibaoka, H. (1984) Gibberellin stabilizes microtubules in onion leaf sheath cells. Protoplasma119, 100–109
Mizuno, K. (1985) In vitro assembly of microtubules from tubulins of several higher plants. Cell Biol. Int. Rep.9, 13–21
Nagata, T., Okada, K., Takebe, I., Matsui, C. (1981) Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Mol. Gen. Genet.184, 161–165
Palevitz, B.A. (1982) The stomatal complex as a model of cytoskeletal participation in cell differentiation. In: The cytoskeleton in plant growth and development, pp.345–376, Lloyd, C.W., ed. Academic Press, London New York
Quader, H., Hofmann, A., Schnepf, E. (1989) Reorganization of the endoplasmic reticulum in epidermal cells of onion bulb scales after cold stress: involvement of cytoskeletal elements. Planta177, 273–280
Rikin, A., Waldman, M., Richmond, A.E., Dovrat, A. (1975) Hormonal regulation of morphogenesis and cold-resistance. J. Exp. Bot.26, 175–183
Rikin, A., Atsmon, D., Gitler, C. (1980) Chilling injury in cotton (Gossypium hirsutum L.): effects of antimicrotubular drugs. Plant Cell Physiol.21, 829–837
Rikin, A., Atsmon, D., Gitler, C. (1983) Quantitation of chill-induced release of a tubulin-like factor and its prevention by abscisic acid inGossypium hirsutum L. Plant Physiol.71, 747–748
Sakiyama, M., Shibaoka, H. (1990) Effects of abscisic acid on the orientation and cold stability of cortical microtubules in epicotyl cells of the dwarf pea. Protoplasma, in press
Smith, J.J., Muldoon, E.P., Lamport, D.T.A. (1984) Isolation of extensin precursors by direct elution of intact tomato cell suspension cultures. Phytochemistry23, 1233–1239
Wick, S.M., Seagull, R.W., Osborn, M., Weber, K., Gunning, B.E.S. (1981) Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J. Cell Biol.89, 685–690
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Akashi, T., Kawasaki, S. & Shibaoka, H. Stabilization of cortical microtubules by the cell wall in cultured tobacco cells. Planta 182, 363–369 (1990). https://doi.org/10.1007/BF02411386
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DOI: https://doi.org/10.1007/BF02411386