Trabecular bone structural variation in biopsy sites of the beagle ilium
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Iliac trabecular bone sites were studied in 2 male and 2 female adult beagles to determine normal structural variability among different sites, and to identify transilial biopsy sites with minimum structural variation. Only sites that provided adequate amounts of trabecular bone were studied. Trabecular bone area and perimeter were measured on microradiographs of ground sagittal sections using a Quantimet 720 image analyzer, and percent bone, perimeter to area ratio, and mean trabecular width were calculated. Over 100% variation was found in the parameters studied among sections from different sites within the same animal. However, variability was minimal among sections obtained more than 2 mm from the lateral or medial cortex within the most caudal sites evaluated. Two sites appropriate for sampling with an 8 mm trephine were identified as being centered over the following points: (a) a point 7 mm caudal to the cranial dorsal spine and 7 mm ventral to the tuber sacrale, and (b) a point 7 mm caudal to the cranial ventral spine and 7 mm dorsal to the tuber coxae. Comparison of data obtained from males and females indicated that sampling variation may also be reduced by using dogs of one sex and by preferential use of males.
Key wordsIlium Trabecular bone Biopsy Variability
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- 1.Rasmussen, H., Bordier, P.: The Physiological and Cellular Basis of Metabolic Bone Disease, pp. 58–59. Williams Wilkins Co., Baltimore, 1974Google Scholar
- 2.Courpron, P., Meunier, P., Bressot, C., Giroux, J. M.: Amount of bone in iliac crest biopsy. In P. J. Meunier (ed.): Bone Histomorphometry, 2nd International Workshop. Armour Montagu, 1977Google Scholar
- 3.Dequeker, J.: Problems in measuring amount of bone: reproducibility, variability, sequential evaluation. In J. Meunier (ed.): Bone Histomorphometry, 2nd International Workshop. Armour Montagu, 1977Google Scholar
- 4.Visser, W. J., Niermans, H. J., Roelofs, J. M. M., Raymakers, J. A., Duursma, S. A.: Comparative morphometry of bone biopsies obtained by two different methods from the right and the left iliac crest. In P. J. Meunier (ed.): Bone Histomorphometry, 2nd International Workshop. Armour Montagu, 1977Google Scholar
- 6.Melsen, F., Melsen, B., Mosekilde, L.: An evaluation of the quantitative parameters applied in bone histology, Acta Pathol. Microbiol. Scand.86:63–69, 1978Google Scholar
- 7.Schenk, R.: Trabecular bone volume in iliac crest biopsies. In Z. F. G. Jaworski (ed.): Proceedings of the First Workshop on Bone Morphometry, pp. University of Ottawa, Ottawa, 1976Google Scholar
- 8.Sokal, K. K., Rohlf, F. J.: Biometry: The Principles and Practice of Statistics in Biological Research. W. H. Freeman and Co., San Francisco, 1969Google Scholar