Calcified Tissue International

, Volume 34, Issue 1, pp 80–85 | Cite as

Effects ofβ-aminoproprionitrile on formation and mineralization of rat bone matrix

  • H. S. Sandhu
  • S. S. Jande


Bones of 4-week-old Sprague-Dawley rats fed a diet containing 0.25%β-aminoproprionitrile (β-APN) for 21 days and injected with3H-proline,3H-tetracycline, and cold tetracycline at 5-, 10-, and 20-day intervals were analyzed for incorporation of3H-proline and3H-tetracycline. Tibial cross sections were examined by fluorescent microscopy to determine the linear bone apposition between intervals and the distribution and intensity of tetracycline bands. The incorporation of3H-proline was similar inβ-APN-treated and control rats. Incorporation of3H-tetracycline, however, was significantly lower inβ-APN-treated rats. Fluorescent microscopy of tibial cross sections showed a comparable amount of linear matrix apposition in control and treated rats. It is suggested thatβ-APN does not inhibit the bone matrix formation but does interfere with its mineralization.

Key words

β-APN Matrix Mineralization Liquid scintillation Fluorescent microscopy 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Martin, G. R., Gross, J., Piez, K. A., Lewis, M. S.: On the intramolecular cross-linking of collagen in lathyritic rats, Biochem. Biophys. Acta53:599–601, 1961CrossRefPubMedGoogle Scholar
  2. 2.
    Piez, K. A.: Cross linking of collagen and elastin, Annu. Rev. Biochem.37:547–569, 1968CrossRefPubMedGoogle Scholar
  3. 3.
    Abramovick, A.: Cleft palate in the fetuses of lathyritic rats and its relation to other structures: nasal septum, tongue, and mandible, Cleft Palate J.9:73–83, 1972Google Scholar
  4. 4.
    Wiley, M. J., Joneja, M. G.: The morphogenesis of B-amino-proprionitrile-induced rib malformation in fetal golden Syrian hamsters, Teratology18:173–186, 1978CrossRefPubMedGoogle Scholar
  5. 5.
    Pratt, R. M. Jr., King, C. T. G.: Inhibition of collagen cross-linking associated with B-aminoproprionitrile-induced cleft palate in rat, Dev. Biol.27:322–328, 1972CrossRefPubMedGoogle Scholar
  6. 6.
    Geiger, B. J., Steenbock, H., and Parsons, H. T.: Lathyrism in rat, J. Nutr.6:437–442, 1933Google Scholar
  7. 7.
    Ponseti, I. V., Shepard, R. S.: Lesions of skeleton and of other mesodermal tissues in rats fed withLathyrus odoratus seeds, J. Bone Joint Surg.36:1031–1058, 1954PubMedGoogle Scholar
  8. 8.
    Selye, H.: Lathyrism, Rev. Can. Biol.16:1–82, 1957PubMedGoogle Scholar
  9. 9.
    Menzies, D. W., Mills, K. W.: The aortic and skeletal lesion of lathyrism in rats on a diet of sweet pea, J. Pathol. Bacteriol.73:223–237, 1957CrossRefGoogle Scholar
  10. 10.
    Gardner, A. F.: Morphologic and histochemical studies of skeletal lesion in rats fed sweet pea (Lathyrus odoratus) seeds, Am. J. Vet. Res.21:298, 1960PubMedGoogle Scholar
  11. 11.
    Tanzer, M. L.: Experimental lathyrism, Int. Rev. Connect. Tissue Res.3:91–112, 1965PubMedGoogle Scholar
  12. 12.
    Barrow, M. V., Simpson, C. F., Miller, E. J.: Lathyrism: a review, Q. Rev. Biol.49:101–128, 1974CrossRefPubMedGoogle Scholar
  13. 13.
    Berquist, E.: A microradiographic study on experimental osteolathyrism in the rat, Acta Soc. Med. Upsal.65:389–395, 1960Google Scholar
  14. 14.
    Berquist, E., Hulth, A.: A study of newly formed bone in lathyritic rats. Microradiographic, tetracycline labelling and microangiographic techniques, Virchows Arch. Pathol. Anat.337:195–204, 1963CrossRefGoogle Scholar
  15. 15.
    Borle, A. B., Karnovsky, M. J., Nichols, G., Jr.: Changes in inorganic composition of tissues in experimental osteolathyrism, Am. J. Physiol197:1224–1228, 1959PubMedGoogle Scholar
  16. 16.
    Mills, B. G., Bavetta, L. A.: Bone collagen dynamics, Clin. Orthop. Rel. Res.57:267–275, 1968Google Scholar
  17. 17.
    Avioli, L. V.: Collagen metabolism, uremia and bone, Kidney Int.4:105–115, 1973PubMedGoogle Scholar
  18. 18.
    Rosenquist, J. B., Baylink, D., Spengler, D. M.: The effect ofβ-APN on bone mineralization, Proc. Soc. Exp. Biol. Med.154:310–313, 1977PubMedGoogle Scholar
  19. 19.
    Frost, H. M., Villaneuva, A. R., Roth, H.: Tetracycline staining of newly forming bone and mineralizing cartilage in vivo, Stain Technol.35:135–138, 1960PubMedGoogle Scholar
  20. 20.
    Harris, W. H., Jackson, R. M., Jowsey, J.: The in vivo distribution of tetracycline in canine bone, J. Bone Joint Surg.44A:1308–1320, 1962Google Scholar
  21. 21.
    Owen, J. J. T.: Localization of tetracycline in bone, Lancet 780–781, 1962Google Scholar
  22. 22.
    Urist, M. R., Ibsen, K. H.: Chemical reactivity of mineralized tissue with oxytetracycline, Arch. Pathol.76:484–496, 1963PubMedGoogle Scholar
  23. 23.
    Steendijk, R.: Studies on the mechanism of the fixation of tetracycline to bone, Acta Anat (Basel)56:368–382, 1964CrossRefGoogle Scholar
  24. 24.
    Ibsen, K. H., Urist, M. R.: The biochemistry and physiology of tetracycline (with special reference to mineralized tissue), Clin. Orthop.32:143–168, 1964PubMedGoogle Scholar
  25. 25.
    Rowland, R.: Exchangeable bone calcium, Clin. Orthop.49:233–248, 1966PubMedGoogle Scholar
  26. 26.
    Treharne, R. W., Brighton, C. T.: The use and possible misuse of tetracycline as a vital stain, Clin. Orthop.140:240–246, 1979PubMedGoogle Scholar
  27. 27.
    Lacroix, P.: Internal remodelling of bones. In G. H. Bourne (ed.): The Biochemistry of Bone, Vol. 3, pp. 119–142. Academic Press, New York, 1971Google Scholar
  28. 28.
    Baylink, D., Stauffer, M., Wergedal, J., Rich, C.: Formation, mineralization, and resorption of bone in vitamin D-deficient rats, J. Clin. Invest.49:1122–1134, 1970PubMedCrossRefGoogle Scholar
  29. 29.
    Frost, H. M.: Tetracycline and fetal bone, Henry Ford Hosp. Med. Bull.13:403–416, 1965Google Scholar
  30. 30.
    Undenfriend, S.: Formation of hydroxyproline in collagen, Science152:1335–1340, 1966Google Scholar
  31. 31.
    Marks, S. C., Jr.: The specificity of the3H-proline incorporation test as a measure of bone matrix formation, Biochem. Biophys. Res. Commun.35:236–246, 1969CrossRefPubMedGoogle Scholar
  32. 32.
    Leblond, C. P., Weinstock, M.: Radioautographic studies of bone formation. In G. H. Bourne (ed.): The Biochemistry and Physiology of Bone, Vol. 3, pp. 181–200. Academic Press, New York, 1971Google Scholar
  33. 33.
    Walker, D. G.: Counteraction to parathyroid therapy in osteopetrotic mice as revealed in plasma calcium level and ability to incorporate3H-proline into bone, Endocrinology79:836–843, 1966PubMedCrossRefGoogle Scholar
  34. 34.
    Frank, R. M., Frank, P.: Autoradiographic quantitative de l'osteogenese en microscopie electronique à l'aide de la proline tritiere, Z. Zellforsch. Mikrosk. Anat.99:121–133, 1969CrossRefPubMedGoogle Scholar
  35. 35.
    Jowsey, J.: Autoradiographic and microradiographic studies of bone. In K. W. McKerna (ed.): The Sex Steroids, pp. 107–147. Appleton-Century-Crofts, New York, 1971Google Scholar
  36. 36.
    Turner, R. T., Schraer, H.: Estrogen-induced sequential changes in avian bone metabolism, Calcif. Tissue Res.24:157–162, 1977CrossRefPubMedGoogle Scholar
  37. 37.
    Golub, L., Stern, B., Glimcher, M., Goldhaber, P.: The inhibition of the maturation of newly synthesized bone collagen by B-aminoproprionitrile in tissue culture, Proc. Soc. Exp. Biol. Med.129:465–469, 1968PubMedGoogle Scholar
  38. 38.
    Steigbigel, N. H., Reed, C. W., Finland, M.: Absorption and excretion of five tetracycline analogues in normal young men, Am. J. Med. Sci.255:296–312, 1968PubMedGoogle Scholar
  39. 39.
    Weinstock, M., Leblond, C. P.: Synthesis, migration and release of precursor collagen by odontoblasts as visualized by radioautography after [3H] proline administration, J. Cell Biol.60:92–127, 1974CrossRefPubMedGoogle Scholar
  40. 40.
    Frost, H. M.: Tetracycline based histological analysis of bone remodeling, Calcif. Tissue Res.3:211–237, 1969CrossRefPubMedGoogle Scholar
  41. 41.
    Chulski, T., Johnson, R. H., Schlagel, C. A., Wagner, J. G.: Direct proportionality of urinary excretion rate and serum level of tetracycline in human subjects, Nature198:450–453, 1963PubMedGoogle Scholar
  42. 42.
    Gordon, A. K., Chung-Ching, L.: Long term testicular response to blinding in rats, Life Sci.14:2179–2188, 1974CrossRefGoogle Scholar
  43. 43.
    Jowsey, J.: The use of the milling machine for preparing bone sections for microradiography, J. Sci. Instrum.32:159–163, 1955CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • H. S. Sandhu
    • 1
  • S. S. Jande
    • 1
  1. 1.Department of AnatomyUniversity of OttawaOttawaCanada

Personalised recommendations