Effects ofβ-aminoproprionitrile on formation and mineralization of rat bone matrix
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Bones of 4-week-old Sprague-Dawley rats fed a diet containing 0.25%β-aminoproprionitrile (β-APN) for 21 days and injected with3H-proline,3H-tetracycline, and cold tetracycline at 5-, 10-, and 20-day intervals were analyzed for incorporation of3H-proline and3H-tetracycline. Tibial cross sections were examined by fluorescent microscopy to determine the linear bone apposition between intervals and the distribution and intensity of tetracycline bands. The incorporation of3H-proline was similar inβ-APN-treated and control rats. Incorporation of3H-tetracycline, however, was significantly lower inβ-APN-treated rats. Fluorescent microscopy of tibial cross sections showed a comparable amount of linear matrix apposition in control and treated rats. It is suggested thatβ-APN does not inhibit the bone matrix formation but does interfere with its mineralization.
Key wordsβ-APN Matrix Mineralization Liquid scintillation Fluorescent microscopy
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