Microstructure and chemical composition of fossil mammalian teeth

  • G. Fosse
  • N. -P. B. Justesen
  • G. B. R. Wesenberg
Article

Summary

Sectioned Cretaceous mammalian teeth were studied by light microscopy and SEM equipment with an electron microprobe. Structures resembling odontoblast processes were found. Their S/Fe ratio was similar to that of rat odontoblast processes, but the amounts of these elements were far higher than in processes of recent teeth. It was concluded that they consisted of pyrite, but their reaction with mercurochrome indicated presence of proteins with SH groups. Fluorescence microscopy indicated traces of collagen in the dentin. In the fossil enamel and dentin the Ca/P ratio was similar to that of recent teeth. Two samples of fossil teeth were analyzed for Pb, Cd, Zn, and Cu content by atomic absorption spectrophotometry. The levels of the latter two elements were normal, whereas that of Cd and especially that of Pb were higher than in laboratory rats. The probability that original protein fractions exist and that the different elements were those of the living animals is discussed.

Key words

Teeth Fossil Elements Protein fractions 

References

  1. 1.
    Wyckoff, R. W. G.: The Biochemistry of Animal Fossils. Scientechnica (Publishers) Ltd., Bristol, 1972Google Scholar
  2. 2.
    Tarlo, L. B., Mercer, J.: A note on the histological study of fossil dentine, Proc. Geol. Soc. Lond.1590:127–128, 1961Google Scholar
  3. 3.
    Isaacs, W. A., Little, K., Currey, J. D., Tarlo, L. B. H.: Collagen and a cellulose-like substance in fossil dentine and bone, Nature197:192, 1963PubMedGoogle Scholar
  4. 4.
    Tarlo, L. B. H.: Psammosteiformes (Agnatha), Paleont. Polon.13:1–135, 1964Google Scholar
  5. 5.
    Ørvig, T.: Microstructure and growth of the dermal skeleton in fossil Actinopterygian fishes: Birgeria and Scanilepsis, Zool. Scripta7:33–56, 1978Google Scholar
  6. 6.
    Pawlicki, R.: Methods of preparation of fossil bone samples for light and transmission electron microscopy, Stain Technol.53:95–102, 1978PubMedGoogle Scholar
  7. 7.
    Lafont, R., Petit-Maire, N.: Les acides amines du collagene: perspectives d'etude d'ossements humains fossiles, Bull. Mem. Soc. Antrop. Paris5: series XIII, 139–142, 1978CrossRefGoogle Scholar
  8. 8.
    Williams, K. M., Smith, G. G.: A critical evaluation of the application of amino acid racemization to geochronology and geothermometry, Orig. Life8:91–144, 1977CrossRefPubMedGoogle Scholar
  9. 9.
    Fosse, G., Justesen, N. -P.B.: Zinc and copper in bone and teeth of mice, Int. J. Environ. Stud.12:111–120, 1978Google Scholar
  10. 10.
    Fosse, G., Justesen, N.-P. B.: Lead in deciduous teeth of Norwegian children, Arch. Environ. Health33:166–175, 1978PubMedGoogle Scholar
  11. 11.
    Fosse, G., Eskildsen, Ø., Risnes, S., Sloan, R. E.: Prism size in tooth enamel of some Late Cretaceous mammals and its value in multituberculate taxonomy, Zool. Scripta7:57–61, 1978CrossRefGoogle Scholar
  12. 12.
    Wesenberg, G. B. R., Fosse, G., Justesen, N.-P. B., Rasmussen, P.: Lead and cadmium in teeth, bone and kidneys of rats with a standard Pb-Cd supply, Int. J. Environ. Stud.14:223–230, 1979Google Scholar
  13. 13.
    Wesenberg, G. B. R., Fosse, G., Rasmussen, P., Justesen, N.-P. B.: The effect of Pb and Cd uptake on Zn and Cu levels in hard and soft tissues of rats, Int. J. Environ. Stud.15:41–48, 1980Google Scholar
  14. 14.
    Fosse, G., Røli, J., Knudsen, H.: A sectioning machine for teeth and other brittle materials, Acta Odontol. Scand.32:299–304, 1974PubMedGoogle Scholar
  15. 15.
    Fosse, G., Holmbakken, N.: Fibrils in marsupial enamel tubules, Z. Zellforsch.115:341–350, 1971CrossRefPubMedGoogle Scholar
  16. 16.
    Cowden, R. R., Curtis, S. K.: Demonstration of proteinbound sulphydryl and disulfide groups with fluorescent mercurials, Histochemistry22:247–255, 1970CrossRefPubMedGoogle Scholar
  17. 17.
    Cantaluppi, G.: Il collagene nei vertebrati fossili: studio applicativo sui bovidi quanternari pavesi, Boll. Soc. Paleontol. Ital.14:55–64, 1975Google Scholar
  18. 18.
    Bennett, H. S.: The demonstration of thiol groups in certain tissues by means of a new colored sulphydryl reagent, Anat. Rec.110:231–247, 1951CrossRefPubMedGoogle Scholar
  19. 19.
    Horowitz, M. G., Klotz, I. M.: Interactions of an azomercurial with proteins, Arch. Biochem. Biophys.63:77–86, 1956CrossRefPubMedGoogle Scholar
  20. 20.
    Engel, M. B., Zerlotti, E.: The histochemical visualization of protein-bound sulphydryl groups with an azomercurial reagent, J. Histochem. Cytochem.12:156–163, 1964PubMedGoogle Scholar
  21. 21.
    Grillo, T. A. I., Baxter-Grillo, D. L.: A fluorescent histochemical method for the detection of tissue disulphide groups, Histochemie18:8–11, 1969CrossRefPubMedGoogle Scholar
  22. 22.
    Allen, D. E., Perrin, D. D.: Robust metal complexes, ferrocenylmethyl carboxyhydrazide and 1-chloromercuriferrocene, as electron-opaque stains for aldehydes and thiol groups, J. Histochem. Cytochem.22:919–928, 1974PubMedGoogle Scholar
  23. 23.
    Sognnaes, R. F.: Microstructure and histochemical characteristics of the mineralized tissues, Ann. N.Y. Acad. Sci.60:545–574, 1955PubMedGoogle Scholar
  24. 24.
    Symons, N. B. B.: Dentinogenesis. In A. E. W. Miles (ed.): Structural and Chemical Organization of Teeth, pp. 295–298. Academic Press, New York, 1967Google Scholar
  25. 25.
    Weill, R.: Détection et localisation des protides sulphydrilés au cours de l'odontogénèse chez le rat blanc, Arch. Oral Biol. [Spec. Suppl.]4:101–106, 1961CrossRefPubMedGoogle Scholar
  26. 26.
    Boyde, A., Reith, E. J.: Qualitative electron probe analysis of secretory ameloblasts and odontoblasts in the rat incisor, Histochemistry50:347–354, 1977CrossRefPubMedGoogle Scholar
  27. 27.
    Fosse, G., Justesen, N.-P. B.: Zinc and copper in deciduous teeth of Norwegian children, Int. J. Environ. Stud.13:19–34, 1978Google Scholar
  28. 28.
    Buczko, Cs., Vas, L.: Effect of climate on chemical composition of fossil bones, Nature269:792–793, 1977CrossRefPubMedGoogle Scholar
  29. 29.
    DeNiro, M. J., Epstein, S.: Influence of diet on the distribution of carbon isotopes in animals, Geochim. Cosmochim. Acta42:495–505, 1978CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • G. Fosse
    • 1
  • N. -P. B. Justesen
    • 1
  • G. B. R. Wesenberg
    • 1
  1. 1.Institute of AnatomyUniversity of BergenBergenNorway

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