Calcified Tissue International

, Volume 33, Issue 1, pp 255–260 | Cite as

Uptake of collagenolytic enzymes by bone cells during isolation from embryonic rat calvaria

  • Jaro Sodek
  • Johan N. M. Heersche


Collagenolytic activity extracted from bone cells, isolated from calvaria with a proteolytic enzyme mixture, was analyzed using14C-labeled collagen and a combination of gel electrophoresis and fluorography. The pattern of collagen degradation products obtained did not include 3/4-fragments, typical of vertebrate collagenases, but was essentially identical to the patterns generated by very dilute samples of the proteolytic enzyme mixture used to isolate the bone cells, and purified collagenase fromClostridium histolyticum (E.C. These and other results strongly suggest that the previously reported collagenolytic activity of bone cells [1] is exogenous in origin.

Key words

Bacterial collagenase Vertebrate collagenase Sodium dodecyl sulfate polyacrylamide gel electrophoresis Fluorography 


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Jaro Sodek
    • 1
  • Johan N. M. Heersche
    • 1
  1. 1.Medical Research Council Group in Periodontal Physiology, Faculty of DentistryUniversity of TorontoTorontoCanada

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