Summary
Rat peritoneal macrophages elicited by injection of thioglycollate were cultured in the absence and presence of non-vital, milled rat bone or latex beads. After 0, 48, 96, and 144 hours exposure to these substances, the levels of aryl sulfatase B were determined. Cells exposed to bone demonstrated a significant (p<.001) time-dependent increase in the specific activity of aryl sulfatase B. After 144 hours the specific activity of aryl sulfatase in cells exposed to bone was 20 fold higher than that in the 144 hour controls. There were no significant differences in the levels of aryl sulfatase B in controls over time, nor were the levels of enzyme in those exposed to latex beads different from controls. We conclude that the specific activity of aryl sulfatase B increases when macrophages resorb bone and suggest that this enzyme could be used as a marker or index for bone resportion.
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Gothlin, G., Ericsson, J.L.E.: On the histogenesis of the cells in fracture callus. Electron microscopic autoradiographic observations in parabiotic rats and studies on labelled monocytes. Virchow's Arch. Abt. B. Zellpath. 12, 318–329 (1973)
Walker, D.G.: Bone resorption restored in osteopetrotic mice by transplants of normal bone marrow and spleen cells. Science 190, 784–785 (1975)
Walker, D.G.: Spleen cells transmit osteopetrosis in mice. Science 190, 785–787 (1975)
Teitelbaum, S., Stewart, C.C., Kahn, A.J.: Rodent peritoneal macrophages as bone resorbing cells. Calcif. Tiss. Int., In Press (1980)
Heersche, J.N.M.: Mechanism of osteoclastic bone resorption: a new hypothesis. Calcif. Res. Int. 26, 81–84 (1978)
Wahl, L.M., Wahl, S.M., Mergenhagen, S.E., Martin, G.R.: Collagenase production by endotoxin activated macrophages. Proc. Nat. Acad. Sci. USA 71, 3598–3601 (1974)
Unkeless, J., Gordon, S., Reich, E.: Secretion of plasminogen activator by stimulated macrophages. J. Exp. Med. 193, 834–850 (1974)
Werb, Z., Gordon, S.: Elastase secretion by stimulated macrophages. J. Exp. Med. 142, 376–377 (1975)
Gibson, W.A., Fullmer, H.M.: Histochemistry of the periodontal ligament. IV. The glycosidases. Periodontics 40, 470–475 (1969)
Dorey, C.K., Bick, K.L.: Ultrastructural analysis of glycosaminoglycan hydrolysis in the rat periodontal ligament. I. Evidence for macrophage involvement in bone remodelling. Calcif. Tiss. Res. 24, 135–141 (1977)
Gibson, W.A., Fullmer, H.M.: Histochemistry of the periodontal ligament. V. The aryl sulfatases. J. Periodont. 41, 102–104 (1970)
Dorey, C.K., Bick, K.L.: Ultrahistochemical analysis of glycosaminoglycan hydrolysis in the rat periodontal ligament. II. Aryl sulfatase and bone resorption. Calcif. Tiss. Res. 24, 143–149 (1977)
Baylink, D., Wegedal, J., Thompson, E.: Loss of protein polysaccharides at sites where bone mineralization is initiated. J. Histochem. Cytochem. 20, 272–292 (1972)
Tudball, N., Davidson, E.A.: Isolation of a novel sulfatase from rat liver. Biochim. Biophys. Acta 171, 113–120 (1968)
Lamberg, S.E., Stoolmiller, A.C.: Glycosaminoglycans. A biochemical and clinical review. J. Invest. Dermatol. 63, 433–449 (1974)
Cohn, Z.A., Benson, B.: The in vitro differentiation of mononuclear phagocytes. II. The influence of serum on granule formation, hydrolase production and pinocytosis. J. Exp. Med. 121, 835–872 (1965)
Wachsmuth, E.D.: Aminopeptidase as a marker for macrophage differentiation. Exp. Cell Res. 96, 409–412 (1975)
Carr, I.: The macrophage. A review of ultrastructure and function. New York, Academic Press (1973)
Engfeldt, B., Hjerpe, A.: Glycosaminoglycans of dentine and predentine. Calcif. Tiss. Res. 10, 152–159 (1973)
Vejlens, L.: Glycosaminoglycans of human bone tissue. I. Pattern of compact bone in relation to age. Calcif. Tiss. Res. 7, 175–190 (1971)
Hjerpe, A., Reinhold, F.D., Engfeldt, B.: The occurrence of chondroitin-6-sulphate in adult human compact bone tissue. Calcif. Tiss. Int. 29, 169–171 (1979)
Fluharty, A.L., Stevens, R.L., Fung, D., Peck, S., Kihara, H.: UDP-N-acetylgalactosamine-4-sulfohydrolase activity of human aryl sulfatase B and its deficiency in the Maroteaux-Lamy syndrome. Biochem. Biophys. Res. Comm. 64, 955–962 (1975)
Matalon, R., Arbogast, B., Justice, P., Brandt, I., Dorfman, A.: Morquio's syndrome: Deficiency of a chondroitin sulfate-N-acetylhexosamine sulfate sulfatase. Biochem. Biophys. Res. Comm. 61, 709–715 (1974)
Wooshman, H., Hardtrodt, W.: Der nachweis einer phosphatempfundlichen sulfatase mit napthol-AS-sulfaten. Histochemie 4, 336–344 (1964)
Milson, D.W., Rose, R.A., Dodgson, K.S.: The specific activity of aryl sulfatase C, a rat liver microsomal enzyme. Biochem. J. 128, 331–336 (1976)
Oyama, V.I., Eagle, H.: Measurement of cell growth in tissue culture with phenol reagent. Proc. Soc. Exp. Biol. Med. 91, 305–307 (1956)
Bowers, W.E., Finkenstaldt, J.T., De Duve, C.: Lysosomes in lymphoid tissue. I. Measurement of hydrolytic activities in whole homogenates. J. Cell Biol. 32, 325–337 (1967)
Werb, Z., Gordon, S.: Secretion of specific collagenase by stimulated macrophages. J. Exp. Med. 142, 346–360 (1975)
Baum, H., Dodson, K.S., Spencer, B.: The assay of aryl sulfatase A and B in human urine. Clin. Chim. Acta 4, 453–455 (1959)
Wergedal, J.E.: Characterization of bone acid phosphatase activity. Proc. Soc. Exp. Biol. Med. 134, 224–247 (1970)
Hammerstrom, L.E., Hanker, J.S., Toverud, S.U.: Cellular differences in acid phosphatase isoenzymes in bones and teeth. Clin. Orthoped. 78, 151–167 (1971)
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Gies, J.P., Dorey, C.K. Stimulation of aryl sulfatase in rat peritoneal macrophages exposed to bone in vitro. Calcif Tissue Int 33, 181–184 (1981). https://doi.org/10.1007/BF02409433
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DOI: https://doi.org/10.1007/BF02409433