Regulation of calcium-binding protein messenger RNA by 1,25-dihydroxycholecalciferol
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1,25-Dihydroxycholecalciferol modulates the synthesis of the intestinal calcium-binding protein. To determine if this effect is due to an increase in calcium-binding protein mRNA activity, we measured total cytoplasmic protein-specific mRNA activity from chick intestine in an in vitro wheat germ translation system. 1,25-Dihydroxy-cholecalciferol enhances calcium-binding protein specific mRNA activity. The sterol does not induce a general increase in mRNA synthesis since the concentration of only a few proteins increased. Thus 1,25-dihydroxycholecalciferol regulates only a subset of genes and specifically affects a transcriptional process involving calcium-binding protein synthesis.
Key wordsCalcium-binding protein Messenger RNA 1,25-Dihydroxycholecalciferol
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- 5.Zerwekh, J.E.: Increased intestinal chromatin template activity, Fed. Proc.747:1976; Federal Association Society of Experimental Biology (abst.)Google Scholar
- 10.Charles, M.A., Marichal, J., Baxter, J.D.: Activation of mRNA for CaBP by 1,25-D3, Clin. Res.25:158A, 1977Google Scholar