Calcified Tissue International

, Volume 33, Issue 1, pp 15–18 | Cite as

Regulation of calcium-binding protein messenger RNA by 1,25-dihydroxycholecalciferol

  • M. Arthur Charles
  • Joseph Martial
  • David Zolock
  • Robert Morrissey
  • John D. Baxter
Article

Summary

1,25-Dihydroxycholecalciferol modulates the synthesis of the intestinal calcium-binding protein. To determine if this effect is due to an increase in calcium-binding protein mRNA activity, we measured total cytoplasmic protein-specific mRNA activity from chick intestine in an in vitro wheat germ translation system. 1,25-Dihydroxy-cholecalciferol enhances calcium-binding protein specific mRNA activity. The sterol does not induce a general increase in mRNA synthesis since the concentration of only a few proteins increased. Thus 1,25-dihydroxycholecalciferol regulates only a subset of genes and specifically affects a transcriptional process involving calcium-binding protein synthesis.

Key words

Calcium-binding protein Messenger RNA 1,25-Dihydroxycholecalciferol 

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • M. Arthur Charles
    • 1
  • Joseph Martial
    • 1
  • David Zolock
    • 1
  • Robert Morrissey
    • 1
  • John D. Baxter
    • 1
  1. 1.Letterman Army Institute of Research, Metabolic Research UnitUniversity of CaliforniaSan FranciscoUSA

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