Summary
The effects of fluoride on human enamel and selachian enameloid in vitro were visualized in TEM and analyzed with electron diffraction. It is demonstrated that under precise pH conditions, inducing concentration balance between F− ions and apatite, calcium fluoride is no longer formed, and crystalline changes occur instead. A secondary growing process, inducing a twofold increase in crystal size, involves all crystal faces, altering the hexagonal symmetry. It is suggested that the mechanism involved is not a dissolution/precipitation process but rather a secondary growth of residual crystallites induced by apatite dissolution.
Similar content being viewed by others
References
McCann, H.G.: Reactions of fluoride ion with hydroxyapatite, J. Biol. Chem.201:247–259, 1953
Trautz, O.R., Zapanta-Legeros, R.: Effects of magnesium on various calcium phosphates, Proc. 40th IADR General Meeting,101, 1962
Ericsson, Y.: The chemistry of the enamel saliva interface, Ala. J. Med. Sci.5:256–266, 1968
Baud, C.A., Bang, S.: Electron probe and X-ray diffraction microanalysis of human enamel treated in vitro by fluoride solution, Caries Res.4:1–13, 1970
Newesely, H.: Factors controlling apatite crystallization with particular reference to the effect of fluoride accompanying ions, Adv. Oral Biol.4:11–42, 1970
Krutchkoff, D.J., Rowe, N.H.: Chemical nature of remineralized flattened enamel surfaces, J. Dent. Res.50:1621–1625, 1971
Wier, D.R., Chien, S.H., Black, C.A.: Transformation of hydroxyapatite to fluorapatite, Soil. Sci. Soc. Am. Proc.36:285–288, 1972
Antila, M.K., Pohto, P.: Fluoride uptake and retention in dental enamel treated with amine fluoride solution, Proc. Finn. Dent. Soc.5:202–207, 1973
Jeansonne, B.G., Feagin, F.F.: Reactions of enamel surfaces and neutral calcium phosphate solutions after combinations of topical APF and Sn F2, J. Dent. Res.52:348–351, 1973
Liang, Z.S., Higushi, W.I.: Kinetics and mechanism of the reaction between hydroxyapatite and fluoride in aqueous acidic media, J. Phys. Chem.77:1704–1710, 1973
Ramsey, A.C., Duff, E.J., Paterson, L., Stuart, J.L.: The uptake of F− by hydroxyapatite at varying pH, Caries Res.7:231–244, 1973
Duff, E.: An infrared and X-ray diffractometric study of the incorporation of fluoride into hydroxyapatite under conditions of the cyclic variations of pH, Arch. Oral Biol.20:763–769, 1975
Magrill, D.S.: Influence of fluoride on the rate of dissolution of hydroxyapatite in acidic buffer solution, Caries Res.9:45–49, 1975
Hercules, D.M., Craig, N.L.: Composition of fluoridated dental enamel studied by X-ray photoelectron spectroscopy (ESCA), J. Dent. Res.55:829–835, 1976
Larsen, J.M., Fejerskov, O., Thylstrup, A.: Microscopical examination of Ca F2 formation on enamel and its transformation to fluorapatite, J. Dent. Res.56 (special issue A):53, 1977
Brown, W.E., Gregory, T.M., Chow, L.C.: Effects of fluoride on enamel solubility and cariostasis, Caries Res.11 (Suppl. 1):118–141, 1977
Daculsi, G., Kerebel, B.: High resolution electron microscope study of human enamel crystallites: size, shape and growth, J. Ultrastruct. Res.65:163–172, 1978
Smales, F.C., Goss, W.P.: Influence of fluoride ions on the morphology of hydroxyapatite crystallites, J. Dent. Res.50:13, 1971
Ingram, G.S., Nash, P.F.: Hydroxyapatite crystal growth, ORCA (Megeve), 1977
Christiansen, J.L., Larsen, J.M., Fejerskov, O.: Remineralization of fluorosed enamel in vitro, J. Dent. Res.56 (special issue A):440, 1977
Montel, G.: Mode d'action du fluor sur le comportement physicochimique du constituant minéral des dents. In: Barmes et al.: Entretiens de Bichat 1976, pp. 12–14, Expansion Scientifique, Paris, 1977
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Daculsi, G., Kerebel, LM. & Kerebel, B. Effects of fluoride on human enamel and selachian enameloid in vitro: A high-resolution TEM and electron diffraction study. Calcif Tissue Int 33, 9–13 (1981). https://doi.org/10.1007/BF02409406
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02409406