The Histochemical Journal

, Volume 28, Issue 4, pp 289–297 | Cite as

Distribution of peptidergic nerve fibres in bullfrog lingual papillae demonstrated by a combination of double immunofluorescence labelling and a multiple dye filter

  • Tatsumi Kusakabe
  • Tadashi Kawakami
  • Michio Ono
  • Hajime Sawada
  • Toshifumi Takenaka


Immunoreactivity of substance P, calcitonin gene-related peptide, vasoactive intestinal polypeptide, neuropeptide Y, and galanin is localized in nerve fibres distributed in the fungiform and filiform papillae of the tongue of the bullfrog,Rana catesbeiana. A combination of indirect double immunofluorescence labelling and a multiple dye filter system clearly demonstrated that all substance P fibres in the connective tissue core of the fungiform and filiform papillae, and within the rim of ciliated cells located on the top of the fungiform papillae showed coexistence with calcitonin gene-related peptide. A few fibres in the epithelial discs, which are located in the centre of the top of the fungiform papillae, showed the immunoreactivity of calcitonin gene-related peptide alone. There were no substance P fibres which showed coexistence with vasoactive intestinal polypeptide, galanin, and neuropeptide Y. In high magnification images, substance P and vasoactive intestinal polypeptide, and substance P and galanin fibres were recognized as two interwined fibres within the same thin nerve bundle. No immunoreactivity of leucine- and methionine-enkephalins can be detected.

These findings suggest that the chemoreceptor function of the bullfrog gustatory organ may be under the control of complicated peptidergic innervation.


Vasoactive Intestinal Polypeptide Ciliated Cell Nerve Bundle Double Immunofluorescence Labelling High Magnification Image 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Tatsumi Kusakabe
    • 1
  • Tadashi Kawakami
    • 2
  • Michio Ono
    • 1
  • Hajime Sawada
    • 1
  • Toshifumi Takenaka
    • 2
  1. 1.Department of AnatomyYokohama City University School of MedicineYokohamaJapan
  2. 2.Department of PhysiologyYokohama City University School of MedicineYokohamaJapan

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