Calcified Tissue International

, Volume 30, Issue 1, pp 73–76 | Cite as

Vitamin K-dependent γ-carboxyglutamic acid formation by mouse renal adenocarcinoma cells (RAG)

  • Hector Pedro Traverso
  • Peter V. Hauschka
  • Paul M. Gallop
Laboratory Investigations


Previous studies have identified γ-carboxyglutamic acid as a constituent of one or more protein(s) synthesized by rat and chicken kidney microsomes in vitro in a vitamin K-dependent post-translational reaction [1]. Incubation of microsomes from a mouse kidney cell line (RAG) with [14C]NaHCO3 results in formation of protein-bound [14C]γ-carboxyglutamic acid. Incorporation is stimulated threefold by addition of the active vitamin K compound 2-methyl, 3-farnesyl, 1,4-naphthoquinone. At least 90% of incorporated, nondialyzable [14C] is situated in the γ-carboxyl group of γ-carboxyglutamic acid residues.

Key words

γ-Carboxyglutamic acid Vitamin K Kidney microsomes Ca2+ metabolism Renal adenocarcinoma 


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Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • Hector Pedro Traverso
    • 1
  • Peter V. Hauschka
    • 1
  • Paul M. Gallop
    • 1
  1. 1.Departments of Orthopaedic Surgery, Oral Biology, and Biological Chemistry, Harvard Schools of Medicine and Denal MedicineChildren's Hospital Medical CenterBostonUSA

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