Advertisement

Calcified Tissue International

, Volume 30, Issue 1, pp 73–76 | Cite as

Vitamin K-dependent γ-carboxyglutamic acid formation by mouse renal adenocarcinoma cells (RAG)

  • Hector Pedro Traverso
  • Peter V. Hauschka
  • Paul M. Gallop
Laboratory Investigations

Summary

Previous studies have identified γ-carboxyglutamic acid as a constituent of one or more protein(s) synthesized by rat and chicken kidney microsomes in vitro in a vitamin K-dependent post-translational reaction [1]. Incubation of microsomes from a mouse kidney cell line (RAG) with [14C]NaHCO3 results in formation of protein-bound [14C]γ-carboxyglutamic acid. Incorporation is stimulated threefold by addition of the active vitamin K compound 2-methyl, 3-farnesyl, 1,4-naphthoquinone. At least 90% of incorporated, nondialyzable [14C] is situated in the γ-carboxyl group of γ-carboxyglutamic acid residues.

Key words

γ-Carboxyglutamic acid Vitamin K Kidney microsomes Ca2+ metabolism Renal adenocarcinoma 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hauschka, P.V., Friedman, P.A., Gallop, P.M., Traverso, H.P.: Vitamin K-dependent γ-carboxyglutamic acid formation by kidney microsomesin vitro, Biochem. Biophys. Res. Commun.71:1207–1213, 1976CrossRefPubMedGoogle Scholar
  2. 2.
    Shah, D.V., Suttie, G.W.: The vitamin K dependentin vitro production of prothrombin, Biochem. Biophys. Res. Commun.60:1397–1402, 1974CrossRefPubMedGoogle Scholar
  3. 3.
    Esmon, C.T., Sadowski, G.A., Suttie, G.W.: A new carboxylation reaction. The vitamin K-dependent incorporation of H14CO3 into prothrombin, J. Biol. Chem.250:4744–4748, 1975PubMedGoogle Scholar
  4. 4.
    Steflo, G., Suttie, G.W.: Vitamin K-dependent formation of γ-carboxyglutamic acid, Annu. Rev. Biochem.46:157–172, 1977CrossRefGoogle Scholar
  5. 5.
    Hauschka, P.V., Lian, J.B., Gallop, P.M.: Direct identification of the calcium-binding amino acid, γ-carboxyglutamate, in mineralized tissue, Proc. Natl. Acad. Sci. U.S.A.72:3925–3929, 1975PubMedGoogle Scholar
  6. 6.
    Price, P.A., Otsuka, A.S., Poser, G.W., Kristaponis, J., Raman, N.: Characterization of a γ-carboxyglutamic acid-containing protein from bone, Proc. Natl. Acad. Sci. U.S.A.73:1447–1451, 1976PubMedGoogle Scholar
  7. 7.
    Traverso, H.P., Gallop, P.M., Hauschka, P.V.: Isolation of a vitamin K-dependent protein from chicken kidney microsomes containing γ-carboxyglutamic acid. XIth International Congress of Biochemistry, Toronto, 1979Google Scholar
  8. 8.
    Lian, J.B., Prien, E.L., Glimcher, M.J., Gallop, P.M.: The presence of protein-bound γ-carboxyglutamic acid in calcium-containing renal calculi, J. Clin. Invest.59:1151–1157, 1977PubMedCrossRefGoogle Scholar
  9. 9.
    Lian, J.B., Skinner, M.S., Glimcher, M.J., Gallop, P.M.: The presence of γ-carboxyglutamic acid in the proteins associated with ectopic calcification, Biochem. Biophys. Res. Commun.73:349–355, 1976CrossRefPubMedGoogle Scholar
  10. 10.
    Fernlund, P.: γ-Carboxyglutamic acid in human urine, Clin. Chim. Acta72:147–155, 1976CrossRefPubMedGoogle Scholar
  11. 11.
    Friedman, P.A., Hauschka, P.V., Shia, M., Wallace, J.K.: Characteristics of the vitamin K-dependent carboxylation system in placenta, Biochim. Biophys. Acta583:261–265, 1979PubMedGoogle Scholar
  12. 12.
    Tuan, R.S.: Vitamin K-dependent γ-glutamyl carboxylase activity in the chick embryonic chorioallantoic membrane, J. Biol. Chem.254:1356–1364, 1979PubMedGoogle Scholar
  13. 13.
    Bell, R.G.: Vitamin K-dependent carboxylation in lung and other extrahepatic tissues, Fed. Proc.38:875, 1979Google Scholar
  14. 14.
    Traverso, H.P., Hauschka, P.V., Gallop, P.M.: Vitamin K-dependent carboxylation in microsomal preparations derived from cultured kidney cells, chick embryo fibroblasts, and pancreas, In Vitamin K Metabolism and Vitamin K-Dependent Proteins, J.W. Suttie, ed., University Park Press, Baltimore, pp. 311–314, 1979Google Scholar
  15. 15.
    Esmon, C.T., Suttie, J.W., Jackson, C.M.: Difference in phospholipid binding between normal and abnormal prothrombin, J. Biol. Chem.250:4095–4099, 1975PubMedGoogle Scholar
  16. 16.
    Nelsestuen, G.L., Lim, T.K.: Equilibrium involved in prothrombin- and blood-clotting factor X-membrane binding, Biochemistry16:4164–4171, 1977CrossRefPubMedGoogle Scholar
  17. 17.
    Munns, T.W., Johnston, M.F.M., Liszewsky, M.K., Olson, R.E.: Vitamin K-dependent synthesis and modification of precursor prothrombin in cultured H-35 hepatoma cells, Proc. Natl. Acad. Sci. U.S.A.73:2803–2807, 1976PubMedGoogle Scholar
  18. 18.
    Lian, J.B., Friedman, P.A.: The vitamin K-dependent synthesis of γ-carboxyglutamic acid by bone microsomes, J. Biol. Chem.253:6623–6626, 1978PubMedGoogle Scholar
  19. 19.
    Nishimoto, S.K., Price, P.A.: Proof that the γ-carboxyglutamic acid-containing bone protein is synthesized in calf bone, J. Biol. Chem.254:437–441, 1979PubMedGoogle Scholar
  20. 20.
    Klebe, R.J., Tchaw-Ren Chen, Ruddle, F.H.: Controlled production of proliferating somatic cell hybrids, J. Cell Biol.45:74–82, 1970CrossRefPubMedGoogle Scholar
  21. 21.
    Friedman, P.A., Shia, M.: Some characteristics of a vitamin K-dependent carboxylating system from rat liver microsomes, Biochem. Biophys. Res. Commun.72:647–654, 1976CrossRefGoogle Scholar
  22. 22.
    Hauschka, P.V.: Quantitative determination of γ-carboxyglutamic acid in proteins, Anal. Biochem.80:212–223, 1977CrossRefPubMedGoogle Scholar
  23. 23.
    Tishler, M., Fieser, L., Wendler, N.: Hydro, oxido and other derivatives of vitamin K1 and related compounds, J. Am. Chem. Soc.62:2866–2871, 1940CrossRefGoogle Scholar
  24. 24.
    Suttie, G.W., Hageman, G.M., Lehrman, S.R., Rich, D.H.: Vitamin K-dependent carboxylase—Development of a peptide substrate, J. Biol. Chem.251:5826–5830, 1976Google Scholar
  25. 25.
    Hauschka, P.V., Reid, M.R.: Vitamin K dependence of a calcium-binding protein containing γ-carboxyglutamic acid in chicken bone, J. Biol. Chem.253:9063–9068, 1978PubMedGoogle Scholar
  26. 26.
    Orloff, J., Berliner, R.W. (eds.): Handbook of Physiology, Section 8: Renal Physiology. American Physiological Society, Washington, D.C., 1973Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • Hector Pedro Traverso
    • 1
  • Peter V. Hauschka
    • 1
  • Paul M. Gallop
    • 1
  1. 1.Departments of Orthopaedic Surgery, Oral Biology, and Biological Chemistry, Harvard Schools of Medicine and Denal MedicineChildren's Hospital Medical CenterBostonUSA

Personalised recommendations