Vitamin K-dependent γ-carboxyglutamic acid formation by mouse renal adenocarcinoma cells (RAG)
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Previous studies have identified γ-carboxyglutamic acid as a constituent of one or more protein(s) synthesized by rat and chicken kidney microsomes in vitro in a vitamin K-dependent post-translational reaction . Incubation of microsomes from a mouse kidney cell line (RAG) with [14C]NaHCO3 results in formation of protein-bound [14C]γ-carboxyglutamic acid. Incorporation is stimulated threefold by addition of the active vitamin K compound 2-methyl, 3-farnesyl, 1,4-naphthoquinone. At least 90% of incorporated, nondialyzable [14C] is situated in the γ-carboxyl group of γ-carboxyglutamic acid residues.
Key wordsγ-Carboxyglutamic acid Vitamin K Kidney microsomes Ca2+ metabolism Renal adenocarcinoma
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