Summary
Previous studies have identified γ-carboxyglutamic acid as a constituent of one or more protein(s) synthesized by rat and chicken kidney microsomes in vitro in a vitamin K-dependent post-translational reaction [1]. Incubation of microsomes from a mouse kidney cell line (RAG) with [14C]NaHCO3 results in formation of protein-bound [14C]γ-carboxyglutamic acid. Incorporation is stimulated threefold by addition of the active vitamin K compound 2-methyl, 3-farnesyl, 1,4-naphthoquinone. At least 90% of incorporated, nondialyzable [14C] is situated in the γ-carboxyl group of γ-carboxyglutamic acid residues.
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Traverso, H.P., Hauschka, P.V. & Gallop, P.M. Vitamin K-dependent γ-carboxyglutamic acid formation by mouse renal adenocarcinoma cells (RAG). Calcif Tissue Int 30, 73–76 (1980). https://doi.org/10.1007/BF02408609
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DOI: https://doi.org/10.1007/BF02408609