Calcified Tissue International

, Volume 29, Issue 1, pp 119–125 | Cite as

Potassium, sodium, and the intracellular fluid space of cells from bone

  • John S. Brand
  • Janet Cushing
  • Thomas Hefley
Laboratory Investigations


Cells enzymatically dispersed from fetal rat calvaria were analyzed for sodium and potassium content and intracellular fluid space (ICF). Even when obtained in comparatively high yield, the cells are damaged by the isolation procedure as evidenced by high sodium and low potassium content immediately after isolation. During a post-incubation period potassium is accumulated and sodium extruded to steady-state levels. Although electrolyte content of cells after recovery did not vary as a function of cell yield, ICF was increased in cells obtained in lower yield, suggesting cell swelling as a result of membrane damage.

The weighted mean values obtained for the best cell preparations were 117 mM K+ and 27 mM Na+. Based on DNA assay of isolated cells and the whole tissue, 20- to 21-day calvaria were found to have an average of 8.1 × 106 cells/calvarium. Combining cell data with analysis of total tissue sodium, potassium, and water, it was concluded that the tissue extracellular sodium is in equilibrium with blood but that the potassium concentration is approximately 5-fold higher than blood levels.

Key words

Potassium Sodium Water space Bone cells 


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  1. 1.
    Bergstrom, W.H., Wallace, W.M.: Bone as a sodium and potassium reservoir, J. Clin. Invest.33:867–873, 1954PubMedCrossRefGoogle Scholar
  2. 2.
    Bergstrom, W.H.: The skeleton as an electrolyte reservoir, Metabolism5:433–437, 1955Google Scholar
  3. 3.
    Edelman, I.S., Leibman, J.: Anatomy of body water and electrolytes, Am. J. Med.27:256–277, 1959CrossRefPubMedGoogle Scholar
  4. 4.
    Norman, N.: The participation of bone in the sodium and potassium metabolism in the rat. I. Simultaneous determination of the exchangeable body sodium and potassium, and the exchangeable and inexchangeable fractions of these ions in bone of the normal rat, Acta Physiol. Scand.57:363–372, 1963CrossRefGoogle Scholar
  5. 5.
    Triffitt, J.T., Terepka, A.R., Neuman, W.F.: A comparative study of the exchange in vivo of major constituents of bone mineral, Calcif. Tissue Res.2:165–176, 1968CrossRefPubMedGoogle Scholar
  6. 6.
    Canas, F., Terepka, A.R., Neuman, W.F.: Potassium and the milieu interiur of bone, Am. J. Physiol.217:117–120, 1969PubMedGoogle Scholar
  7. 7.
    Scarpace, P.J., Neuman, W.F.: The blood:bone disequilibrium. I. The active accumulation of K+ into the bone extracellular fluid, Calcif. Tissue Res.20:137–149, 1976Google Scholar
  8. 8.
    Neuman, W.F., Ramp, W.K.: The concept of a bone membrane: some implications. In G. Nichols, R.H. Wasserman, (eds.): Cellular Mechanisms for Calcium Transfer and Homeostasis, pp. 197–206. Academic Press, New York, 1971Google Scholar
  9. 9.
    Dittmer, D.S. (ed): Blood and Other Body Fluids. Fed. Am. Soc. Exp. Biol. Med., Washington, D.C., 1961Google Scholar
  10. 10.
    Kolb, H.A., Adam, G.: Regulation of ion permeabilities of isolated rat liver cells by external calcium concentration and temperature, J. Membrane Biol.26:121–151, 1976CrossRefGoogle Scholar
  11. 11.
    Dziak, R., Brand, J.S.: Calcium transport in isolated bone cells. I. Bone cell isolation procedures, J. Cell. Physiol.84:75–84, 1974CrossRefPubMedGoogle Scholar
  12. 12.
    Carter-Su, C., Kimmich, G.A.: The membrane potential as a determinant of monosaccharide transport in ATP-depleted isolated intestinal epithelial cells, Am. J. Physiol. (in press)Google Scholar
  13. 13.
    Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid, Biochem. J.62:315–323, 1956PubMedGoogle Scholar
  14. 14.
    Peck, W.A., Birge, S.J., Fedak, S.A.: Bone cells: biochemical and biological studies after enzymatic isolation, Science146:1476–1477, 1964PubMedGoogle Scholar
  15. 15.
    Rodan, S.B., Rodan, G.A.: The effect of parathyroid hormone and thyrocalcitonin on the accumulation of cyclic adenosine 3′:5′-monophosphate in freshly isolated bone cells, J. Biol. Chem.249:3068–3074, 1974PubMedGoogle Scholar
  16. 16.
    Wong, G., Cohn, D.V.: Separation of parathyroid hormone and calcitonin-sensitive cells from non-responsive bone cells, Nature252:713–715, 1974CrossRefPubMedGoogle Scholar
  17. 17.
    Baur, H., Kasparek, S., Pfaff, E.: Criteria of viability of isolated liver cells, Hoppe-Seyler’s Z. Physiol. Chem.356:827–838, 1975PubMedGoogle Scholar
  18. 19.
    Weinger, J.M., Holtrup, M.E.: An ultrastructural study of bone cells: the occurrence of microtubules, microfilaments and tight junctions, Calcif. Tissue Res.14:15–29, 1974CrossRefPubMedGoogle Scholar
  19. 20.
    Davis, W.L., Matthews, J.L., Martin, J.H., Kennedy, J.W., III, Talmage, R.V.: The endosteum as a functional membrane. In R.V. Talmage, M. Owen, J.A. Parsons, (eds.): Calcium Regulating Hormones, pp. 275–283. Excerpta Medica, Amsterdam, 1975Google Scholar
  20. 21.
    McQuade, A., Crewther, W.: Peptide substrates for a proteinase ofClostridium histolyticum, Biochem. Biophys. Acta167:619–620, 1968PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • John S. Brand
    • 1
  • Janet Cushing
    • 1
  • Thomas Hefley
    • 1
  1. 1.Department of Radiation Biology and BiophysicsUniversity of Rochester Medical CenterRochesterUSA

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