Annals of Biomedical Engineering

, Volume 12, Issue 4, pp 335–346 | Cite as

Platelet adhesion determination in whole blood using a simple stagnation flow method

  • Yaacov Berkovitch
  • Abraham Marmur
  • Gerald J. Brook
  • Michael Aviram


A simple stagnation flow method for the determination of platelet adhesivity has been developed. The sensitivity of the method to its main operational parameters was tested, resulting in recommended standard flow conditions. The method was shown to be dependent on the surface properties of the test slide, on which platelets are deposited, thus proving its suitability to elucidate variations in the surface interaction of the platelets. Normal values were established for males and females.


Platelet adhesion Stagnation flow 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Baier, R.E. and R.C. Dutton. Initial events in interactions of blood with a foreign surface.J. Biomed. Mater. Res. 3:191–206, 1969.CrossRefPubMedGoogle Scholar
  2. 2.
    Born, G.V.R. Fluid mechanical and biochemical interactions in haemostasis.Br. Med. Bull. 33:193–197, 1977.PubMedGoogle Scholar
  3. 3.
    Born, G.V.R. and M. Hume. Effects of the numbers and sizes of platelet aggregates on the optical density of plasma.Nature 215:1027–1029, 1967.PubMedGoogle Scholar
  4. 4.
    Dutton, R.C., R.E. Baier, R.L. Dedrick, and R.L. Bowman. Initial thrombus formation on foreign surfaces.Trans. Am. Soc. Artif. Intern. Organs 4:57–62 (1968).Google Scholar
  5. 5.
    George, J.N. Direct assessment of platelet adhesion to glass: A study of the force of interaction and the effects of plasma and serum factors, platelet concentration and modification of the glass surface.Blood. 40:862–869, 1972.PubMedGoogle Scholar
  6. 6.
    Goldsmith, H.L. The flow of model particles and blood cells and its relation to thrombogenesis. InProgress in Hemostasis and Thrombosis, edited by T.H. Spaet. Grune & Stratton, 1972, Vol. 1, pp. 97–139.Google Scholar
  7. 7.
    Gross, J. Studies on the formation of collagen: 1. Properties and fractionation of neutral salt extracts of normal guinea pig connective tissue.J. Exp. Med. 247:107–113, 1958.Google Scholar
  8. 8.
    Hellem, A.J. The adhesiveness of human blood platelets in vitro.Scand. J. Clin. Lab. Invest. Suppl. 51:117, 1960.Google Scholar
  9. 9.
    Lelah, M.D. and A. Marmur. Wettability of soda lime glass: The effect of cleaning procedures.Ceramic Bull. 58:1121–1124, 1979.Google Scholar
  10. 10.
    Levich, V.G.Physicochemical Hydrodynamics. Prentice-Hall, Inc., 1962.Google Scholar
  11. 11.
    Marmur, A. and E. Ruckenstein. Physicochemical aspects of platelet adhesion and thrombogenesis. InAdvances in Biomedical Engineering, edited by D.O. Cooney., Marcel Dekker Inc., pp. 341–390, 1980.Google Scholar
  12. 12.
    Petschek, H., D. Adamis, and A.R. Kantrowitz. Stagnation flow thrombus formation.Trans. Am. Soc. Artif. Intern. Organs 14:256–260 1968.PubMedGoogle Scholar
  13. 13.
    Ruckenstein, E., A. Marmur, and S.R. Rakower. Sedimentation and adhesion of platelets onto a horizontal glass surface.Thromb. Haemost. 36:334–342 1976.PubMedGoogle Scholar
  14. 14.
    Salzman, E.W. Measurements of platelet adhesiveness. Progress report.Thromb. Diath. Haemorrh. Suppl. 26:303–312, 1967.Google Scholar
  15. 15.
    Simmon, K.Y., J.G. Latour, B. Marchandise, and M. Bois. Shear stress-induced changes in platelet reactivity.Thromb. Haemost. 40:551–560 1979.Google Scholar
  16. 16.
    Tangen, O., H.J. Berman, and P. Murphy. Gel filtration: A new technique for separating blood platelets from plasma.Thromb. Diath. Haemorrh. 25:268–278, 1971.PubMedGoogle Scholar
  17. 17.
    Turitto, V.T., A.M. Benis, and E.F. Leonard. Platelet diffusion in flowing blood.Ind. Eng. Chem. Fundam. 11:216–223, 1972.CrossRefGoogle Scholar
  18. 18.
    Turitto, V.T. and E.F. Leonard. Platelet adhesion to a spinning surface.Trans. Am. Soc. Artif. Intern. Organs 18:348–354, 1972.PubMedGoogle Scholar
  19. 19.
    Turitto, V.T., H.J. Weiss, and H.R. Baumgartner. The effect of shear on platelet interaction with subendothelium exposed to citrated human blood.Microvasc. Res. 19:352–365, 1980.CrossRefPubMedGoogle Scholar
  20. 20.
    White, F.M.Viscous Fluid Flow. New York: McGraw-Hill, 1974.Google Scholar

Copyright information

© Pergamon Press Ltd. 1985

Authors and Affiliations

  • Yaacov Berkovitch
    • 1
  • Abraham Marmur
    • 1
    • 2
  • Gerald J. Brook
    • 1
    • 3
    • 4
  • Michael Aviram
    • 4
  1. 1.Department of Biomedical EngineeringTechnion-Israel Institute of TechnologyHaifa
  2. 2.Department of Chemical EngineeringTechnion-Israel Institute of TechnologyHaifa
  3. 3.Department of MedicineTechnion-Israel Institute of TechnologyHaifa
  4. 4.Lipid Research LaboratoryRambam Medical CenterHaifaIsrael

Personalised recommendations