, Volume 4, Issue 4, pp 220–226 | Cite as

Neuropeptide-containing nerve fibers in the pharynx of the rabbit

  • Rolf Uddman
  • Olle Ekberg
  • Lars Malmberg
  • Pia Borgström
  • Gustaf Fernström
  • Jörgen Ekström
  • Frank Sundler


The distribution of peptide-containing nerve fibers in the pharyngeal region of rabbits was studied by immunocytochemistry. Neuropeptide Y (NPY)-containing fibers were numerous around blood vessels and moderate in number among bundles of striated muscle fibers. A few NPY-containing fibers were seen around seromucous glands and beneath the epithelium. Nerve fibers containing vasoactive intestinal peptide (VIP) were numerous around seromucous glands and moderate in number around blood vessels, bundles of muscle, and in the subepithelial layer. A few nerve fibers containing substance P (SP) were seen around blood vessels, seromucous glands, among bundles of muscle, and in the subepithelial layer. Nerve fibers containing calcitonin gene-related peptide (CGRP) were numerous. They were distributed close to blood vessels, among bundles of muscle, in the subepithelial layer, and within the epithelium. A conspicuous finding was the occurrence of CGRP within motor end plates of striated muscle.

Key words

Pharynx, neuropeptides Immunocytochemistry, pharynx 


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  1. 1.
    Bosma JF: Physiology of the mouth, pharynx and esophagus. In Paparella MA, Shumrik MA (eds):Otolaryngoldogy 1980, pp 319–345Google Scholar
  2. 2.
    Bosma JF, Donner MW, Tanaka E, Robertson D: Anatomy of the pharynx pertinet to swallowing.Dysphagia 1:23–33, 1986Google Scholar
  3. 3.
    Uddman R, Sundler F: Neuropeptides in the airways: a review.Am Rev Respir Dis 136: 3–8, 1987Google Scholar
  4. 4.
    Aggestrup S, Uddman R, Jensen SL, Sundler F, Schaffalitzky de Muckadell O, Holst JJ, Håkanson R, Sörensen HR: Regulatory peptides in the lower esophageal sphincter of man.Regul Pept 10: 167–178, 1985CrossRefPubMedGoogle Scholar
  5. 5.
    Ekberg O, Malmberg L, Borgström PS, Ekström J: Neuropharmacological in vitro studies on the pharynx and cervical esophagus in the rabbit. To be published.Google Scholar
  6. 6.
    Coons AH, Leduc EH, Connolly JM: Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.J Exp Med 102: 49–60, 1955CrossRefPubMedGoogle Scholar
  7. 7.
    Dutta CR, Basmajian JV: Gross and histological structure of the pharyngeal constrictors in the rabbit.Anat Rec 137: 127–134, 1960CrossRefGoogle Scholar
  8. 8.
    Nishimura T, Takasu T: The adrenergic innervation in the esophagus and respiratory tract of the rabbit.Acta Otolaryngol 67: 444–452, 1969PubMedGoogle Scholar
  9. 9.
    Ekblad E, Edvinsson L, Wahlestedt C, Uddman R, Håkanson R, Sundler F: Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers.Regul Pept 8: 225–235, 1984CrossRefPubMedGoogle Scholar
  10. 10.
    Grunditz T, Ekman R, Håkanson R, Sundler F, Uddman R: Neuropeptide Y and vasoactive intestinal peptide coexist in thyroid nerve fibers emanating from the thyroid ganglion.Regul Pept 23: 193–208, 1988CrossRefPubMedGoogle Scholar
  11. 11.
    Håkanson R, Sundler F: The role of peptide messengers in the neuroendocrine system: hormones, neurotransmitters or neuromodulators. In Shou JS, Geisler A, Norn S (eds):Drug Receptors and Dynamic Processes in Cells. Copenhagen: Munksgaard, 1986, pp 62–89Google Scholar
  12. 12.
    Said S,Vasoactive Intestinal Peptide. Advances in Peptide Hormone Research Series. New York: Raven Press, 1982Google Scholar
  13. 13.
    Ohhashi T, Jacobowitz DM: Effects of calcitonin gene-related peptide on neuromuscular transmission in the isolated rat diaphragm.Peptides 9: 613–617, 1988CrossRefPubMedGoogle Scholar
  14. 14.
    Fontaine B, Klarsfeld A, Hökfelt T, Changeux J-P: Calcitonin gene-related peptide, a peptide present in spinal cord motoneurons, increases the number of acetylcholine receptors in primary cultures of chick embryo myotubes.Neurosci Lett 71: 59–65, 1986CrossRefPubMedGoogle Scholar
  15. 15.
    Rodrigo J, Polak JM, Fernandez L, Ghatei MA, Mulderry P, Bloom SR: Calcitonin gene-related peptide immunoreactive sensory and motor nerves of the rat, cat, and monkey esophagus.Gastroenterology 88: 444–451, 1985PubMedGoogle Scholar
  16. 16.
    Gibbins IL, Furness JB, Costa M, McIntyre I, Hillyard CJ, Girgis S: Co-localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous vascular and visceral sensory neurons of guinea-pigs.Neurosci Lett 57: 125–130, 1985CrossRefPubMedGoogle Scholar
  17. 17.
    Lee Y, Takani K, Kawai Y, Girgis S, Hillyard C, MacIntyre I, Emson PC, Tohyama M: Distribution of calcitonin gene-related peptide in the rat peripheral nervous system with reference to its coexistence with substance P.Neuroscience 15: 1227–1237, 1985CrossRefPubMedGoogle Scholar
  18. 18.
    Terenghi G, Polak JM, Rodrigo J, Mulderry PK, Bloom SR: Calcitonin gene-related peptide-immunoreactive nerves in the tongue, epiglottis and pharynx of the rat: occurrence, distribution and origin.Brain Res 365: 1–14, 1986CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • Rolf Uddman
    • 1
  • Olle Ekberg
    • 2
  • Lars Malmberg
    • 3
  • Pia Borgström
    • 2
  • Gustaf Fernström
    • 1
  • Jörgen Ekström
    • 3
  • Frank Sundler
    • 4
  1. 1.Department of OtolaryngologyMalmö General HospitalMalmöSweden
  2. 2.Department of Diagnostic RadiologyMalmö General HospitalMalmö
  3. 3.Department of PhysiologyUniversity of LundLundSweden
  4. 4.Department of Medical Cell ResearchUniversity of LundLundSweden

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