Summary
Studies from our laboratory revealed that direct application of physical strain (PS) to cultured bone cells stimulated synthesis of prostaglandin E2 (PGE2) in a specific population of cells. We found that PGE2 induced the cellular production of cAMP in several bone cell types, whereas the induction of DNA synthesis was limited to osteoblastlike cells. Indirect evidence indicated that PS induced the osteoblastlike cells to synthesize PGE2. Other cell types, for example, chondrocytes, when activated by PS, can be induced to produce cAMP and induced DNA synthesis not mediated by PGE2. We have also found that electric stimulation of different populations of bone cells is specifically induced by a certain intensity of the electric field. It seems that electric stimulus circumvents the PGE2 effect and triggers the adenyl cyclase system in the cell directly. The electric field also induces DNA synthesis not via PGE2 production.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Glucksman A (1938) Studies on bone mechanisms in vitro. I. Influence of pressure on orientation of structure. Anat Rec 72:97–113
Glucksman A (1942) The role of mechanical stress in bone formation in vitro. J Anat 76:231–239
Rodan GA, Mensi T, Harvey A (1975) A quantitative method for the application of compressive forces to bone in tissue culture. Calcif Tissue Res 18:125–131
Rodan GA, Bourret LA, Harvey A, Mensi T (1975) Cyclic AMP and cyclic GMP: mediators of mechanical effects on bone remodelling. Science 189:467–468
Rodan GA (1981) Mechanical and electrical effects on bone and cartilage cells: translation of the physical signal into a biological message. In: Barrer HG (ed) Orthodontics, The State of The Art, The University of Pennsylvania Press, Philadelphia, pp 315–322
Somjen D, Binderman I, Berger E, Harell A (1980) Bone remodelling induced by physical stress is prostaglandin E2 mediated. Biochim Biophys Acta 627:91–100
Binderman I, Somjen D, Shimshoni Z, Harell A (1982) The role of prostaglandins (PGE2) in bone remodelling induced by physical forces. In: Menczel, Maikin and Robin (eds) Proceedings of International Symposium on Osteoporosis, John Wiley & Sons, New York, pp 195–199
Somjen D, Korenstein R, Fischler H, Binderman I (1982) Effects of electric field intensity on the responses of cultured bone cells to parathyroid and prostaglandin E2. In: Silberman and Slavkin (eds) Current advances in skeletogenesis: development, biomineralization, mediators and metabolic disease, Excerpta Medica, Amsterdam, Oxford, Princeton, pp 412–416
Binderman I, Duskin D, Harell A, Sachs L, Katchalsky E (1974) Formation of bone tissue in culture from isolated bone cells. J Cell Biol 61:427–439
Shimshoni Z, Binderman I, Fine N, Somjen D (in press) Biochemical characterization of cells derived from the condyle head of young rats. Arch Oral Biol
Luben RA, Wong GL, Cohn DV (1976) Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts. Endocrinology 99:526–534
Wong GL, Luben RA, Cohn DV (1977) 1,25 dihydroxycholecalciferol and parathormone: effects on isolated osteoclast-like and osteoblast-like cells. Science 197:663–665
Charlier JP, Petrovic A (1967) Recherches sur la mandibule det rat en culture d'oranges: le cartilage condylien a-t-il un potentiel de croissance independant? Orthodond France 38:165
Fukuda E, Yasuda I (1957) On the piezoelectric effect of bone. J Physiol Soc Japan 12:1158
Korenstein R, Somjen D, Laub F, Danon A, Fischler H, Binderman I (1983) Pulsed external electric fields are mitogens for bone cells. In: Oplatka and Balaban (eds) Biological structures and coupled flows, Academic Press, New York and Philadelphia, pp 401–411
Somjen D, Shimshoni Z, Levy J, Korenstein R, Fischler H, Binderman I (1983) Stimulation of cell cultures by different electric field intensities is cell specific (Abstract), XVIIIth European Symposium on Calcified Tissue, Davos, Switzerland
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Binderman, I., Shimshoni, Z. & Somjen, D. Biochemical pathways involved in the translation of physical stimulus into biological message. Calcif Tissue Int 36 (Suppl 1), S82–S85 (1984). https://doi.org/10.1007/BF02406139
Issue Date:
DOI: https://doi.org/10.1007/BF02406139