Failure to detect an amorphous calcium-phosphate solid phase in bone mineral: A radial distribution function study
- 176 Downloads
X-ray diffraction radial distribution function analysis was used to determine if a significant amount of an amorphous solid phase of calcium phosphate exists in bone, and if so, whether the amount varies as a function of age and maturation. Unfractionated cortical bone from embryonic and posthatch chicks of various ages and a low-density fraction of embryonic bone were studied. No evidence was found for the presence of an amorphous solid phase of calcium phosphate in any of the samples studied, including the recently deposited bone mineral of the low density fraction of embryonic bone. As little as 12.5% of synthetic amorphous calcium phosphate (ACP) added to bone was readily detected by the radial distribution function technique used. The results clearly indicate that the concept that ACP is the initial solid mineral phase deposited in bone, and the major mineral constituent of young bone is no longer tenable. The concept does not provide an accurate description of the nature of the initial bone mineral deposited, or the changes that occur with maturation, nor can it acount for the compositional and X-ray diffraction changes that the mineral component undergoes during maturation and aging.
Key wordsBone Mineral Amorphous calcium phosphate X-ray diffraction Radial distribution function
Unable to display preview. Download preview PDF.
- 1.Glimcher MJ, (1976) The composition, structure and organization of bone and other mineralized tissues and the mechanisms of calcification. In: Greep RO, Astwood EB, (eds) Handbook of physiology: endocrinology, Vol 7, Am Physiol Soc, Washington, D.C., pp 25–113Google Scholar
- 3.Woodward HQ (1964) The composition of human cortical bone. Clin Orthop 37:187–193Google Scholar
- 5.Eanes ED, Harper RA, Gillessen IH, Posner AS (1966) An amorphous component in bone mineral. In: Gillard PJ, van der Hoff A, Steendyk R (eds) 4th Eur Symp Calcif Tissues, Excerpta Med, Amsterdam, pp 24–26Google Scholar
- 6.Termine JD (1966) Amorphous calcium phosphate: the second mineral of bone. PhD Thesis, Cornell UniversityGoogle Scholar
- 9.Warren BE (1969) X-ray diffraction, Addison-Wesley, Reading, Mass., pp 116–145Google Scholar
- 14.Klug HP, Alexander LE (1974) X-Ray diffraction procedures for polycrystalline and amorphous materials, 2nd ed, John Wiley and Sons, New York, pp 677–690Google Scholar
- 23.Kaplow R, Strong SL, Averbach BL (1966) Local order in liquid alloys. In: Cohen JB, Hilliard JE (eds) Local atomic arrangement studied by x-ray diffraction, Gordon and Breach, New York, pp 159–177Google Scholar
- 27.Betts F, Posner AS (1974) A structural model for amorphous calcium phosphate. Trans Am Cryst Assoc 10:73–84Google Scholar
- 29.Quinaux M, Richelle LJ (1967) X-ray diffraction and infrared analysis of bone specific gravity fraction in the growing rat. Israel J Med Sci 3:677–690Google Scholar
- 31.Baud CA, Pouezat JA, Tochon-Danguy HJ (1976) Quantitative analysis of amorphous and crystalline bone mineral in women with osteoporosis. In: Nielsen SP, Hjrting-Hansen E (eds) Proc. 11th Eur Symp Calcif Tissue, Elsinore 1975, Fadl, CopenhagenGoogle Scholar