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Calcified Tissue International

, Volume 35, Issue 1, pp 821–823 | Cite as

Amorphous calcium phosphate in casein micelles of bovine milk

  • T. C. A. McGann
  • R. D. Kearney
  • W. Buchheim
  • A. S. Posner
  • F. Betts
  • N. C. Blumenthal
Rapid Communication

Summary

The calcium phosphate remaining after hydrazine deproteination of casein micelles isolated from bulk skim milk exhibits under the electron microscope a very fine and uniform granularity being formed by small subunits with a true diameter of approximately 2.5 nm. This material, which is about 10 percent by weight citrate, termed calcium phosphate citrate (CPC) complex, also contains Mg and Zn at molar ratios of 0.03 and 0.003 respectively. Radial distribution function (RDF) and infrared analyses show that CPC is a Mg-containing amorphous calcium phosphate (ACP) similar to synthetic and cytoplasmic ACP. Presence of CPC in casein micelles as an amorphous colloid bonded with phosphoproteins provides the means for storing in milk large amounts of Ca (16 mM) and Pi (10 mM) in a readily utilizable form but at a higher ion concentration than found in biological solutions.

Key Words

Milk-ACP-Citrate-Mg-Zn 

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Copyright information

© Springer-Verlag New York Inc. 1983

Authors and Affiliations

  • T. C. A. McGann
    • 1
  • R. D. Kearney
    • 1
  • W. Buchheim
    • 2
  • A. S. Posner
    • 3
  • F. Betts
    • 3
  • N. C. Blumenthal
    • 3
  1. 1.Agricultural Institute, Moorepark Research Centre, FermoyCo. CorkRepublic of Ireland
  2. 2.Institut fuer Chemie Und PhysikBundesanstalt fuer MilchforschungKielFederal Republic of Germany
  3. 3.Hospital For Special SurgeryCornell University Medical CollegeNew YorkUSA

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