Amorphous calcium phosphate in casein micelles of bovine milk
- 203 Downloads
The calcium phosphate remaining after hydrazine deproteination of casein micelles isolated from bulk skim milk exhibits under the electron microscope a very fine and uniform granularity being formed by small subunits with a true diameter of approximately 2.5 nm. This material, which is about 10 percent by weight citrate, termed calcium phosphate citrate (CPC) complex, also contains Mg and Zn at molar ratios of 0.03 and 0.003 respectively. Radial distribution function (RDF) and infrared analyses show that CPC is a Mg-containing amorphous calcium phosphate (ACP) similar to synthetic and cytoplasmic ACP. Presence of CPC in casein micelles as an amorphous colloid bonded with phosphoproteins provides the means for storing in milk large amounts of Ca (16 mM) and Pi (10 mM) in a readily utilizable form but at a higher ion concentration than found in biological solutions.
Unable to display preview. Download preview PDF.
- 1.Pyne, G.T., McGann, T.C.A.: The colloidal phosphate of milk. II. Influence of citrate. J. Dairy Res. 27: 9–17, 1960.Google Scholar
- 2.McGann, T.C.A., Pyne, G.T.: The colloidal phosphate of milk. III. Nature of its association with casein.Google Scholar
- 4.Lehninger, A.L.: Mitochondria and biological mineralization processes: An exploration. Horizons Biochem. Biophys.4: 1–30, 1977.Google Scholar
- 5.Betts, F., Posner, A.S.: A structural model for ACP. Trans. Amer. Cryst. Assoc.10: 73–84, (1974).Google Scholar
- 7.Buchheim, W.: A comparison of the microstructure of dried milk products by freeze-fracturing powder suspensions in non-aqueous media. Scanning Electron Microsc. 111: 493–502, 1981.Google Scholar
- 14.Knoop, A.M., Frede, E., Precht, D.: Die submikroskopische struktur naturlicher und kunstlicher casein micellen. Milchwissenschaft.34: 129–131, 1979.Google Scholar
- 23.Blumenthal, N.C., Betts, F., Posner, A.S.: Effect of carbonate and biological macromolecules on formation and properties of hydroxyapatite. Calcif. Tiss. Res. 18: 18–90, 1975.Google Scholar