Calcified Tissue International

, Volume 35, Issue 1, pp 602–608 | Cite as

Effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) upon the kinetics of bone resorption and bone formation at the whole bone level in prelabelled chicks

  • LeRoy Klein
  • Kam M. Wong
Laboratory Investigations


Chicks chronically prelabelled with45Ca,3H-tetracycline, and3H-proline were used to measure the weekly effect of EHDP (5 mg P/kg for 28 days) on bone turnover at the whole bone level in vivo. Direct measurements were made of cortical bone resorption (loss of3H-tetracycline and3H-collagen from whole femur, blood-bone ratio of45Ca), skeletal collagen formation and bone mineralization (collagen and calcium mass per whole femur, respectively).

Chicks were sacrificed after 5, 14, 21 and 28 days of EHDP administration. By five days of treatment, EHDP caused a greater inhibition of bone mineralization (86%) than bone resorption (30–39%) without affecting skeletal collagen mass. The blood-bone ratio of45Ca decreased 34%, which was similar in degree to the inhibition of3H-tetracycline loss (30%) and3H-collagen loss (39%). Almost complete inhibition of bone resorption and bone mineralization occurred by 14 days of treatment without effects on skeletal collagen mass. No additional effect was seen at 21 and 28 days of EHDP treatment. In chicks, EHDP inhibits almost completely bone mineralization (bone formation) and cortical bone resorption without affecting skeletal collagen mass.

Key words

Diphosphonate EHDP Bone resorption Bone formation Chicks 


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Copyright information

© Springer-Verlag New York Inc. 1983

Authors and Affiliations

  • LeRoy Klein
    • 1
  • Kam M. Wong
    • 1
  1. 1.Departments of Orthopaedics and Oral Biology, Schools of Medicine and DentistryCase Western Reserve UniversityClevelandUSA

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