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Autoradiographic localization of target cells for 1α, 25-dihydroxyvitamin D3 in bones from fetal rats

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Summary

Thaw-mount autoradiographic studies after injection of3H-1,25-D3 were conducted on 18-and 20-day-old rat fetuses. In maxillary bones, ribs, and tibia, nuclear concentration of radioactivity was found in osteoprogenitor cells and osteoblasts. Osteocytes and chondrocytes in epiphyseal plates were either unlabeled or weakly labeled. In competition experiments, nuclear concentration of radioactivity was blocked by the injection of a high dose of nonradioactive 1,25-D3 prior to the administration of the labeled hormone, but not by a similar dose of nonradioactive 25-D3. The results are interpreted as indicating that osteoprogenitor cells and osteoblasts are target cells for the direct action of 1,25-D3 on fetal bone.

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Authors and Affiliations

  1. Department of Anatomy, University of Ottawa, KIN 9A9, Ottawa, Ontario, Canada

    R. Narbaitz

  2. Department of Anatomy, University of North Carolina, Chapel Hill, North Carolina, USA

    W. E. Stumpf, M. Sar & S. Huang

  3. Department of Biochemistry, University of Wisconsin, Madison, Wisconsin, USA

    H. F. DeLuca

Authors
  1. R. Narbaitz
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  2. W. E. Stumpf
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  3. M. Sar
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  4. S. Huang
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  5. H. F. DeLuca
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Narbaitz, R., Stumpf, W.E., Sar, M. et al. Autoradiographic localization of target cells for 1α, 25-dihydroxyvitamin D3 in bones from fetal rats. Calcif Tissue Int 35, 177–182 (1983). https://doi.org/10.1007/BF02405028

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  • DOI: https://doi.org/10.1007/BF02405028

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