Biochemical analysis of related, independently arising histocompatibility mutants: bm17 and KB-98 enlarge the “bg series” of H-2Kb mutants
- 10 Downloads
The “bg” series of MHC mutations is the most prevalent type of mutations of Kb in C57BL/6 mice screened by reciprocal tail skin grafting. The basis for identification of this series of mutations is the incompatibility of grafts between the parental B6 and the mutant. This series takes the longest to reciprocally reject the skin grafts. The series can be subdivided into “bg 1” and “bg 2” groups based on Kb-restricted recognition of virus-infected mutant target cells. The biochemical basis for these mutations are amino acid substitutions at residues 116 and 121 of the Kb transplantation antigen. These substitutions do not alter monoclonal antibody binding sites. The structural basis of MAb binding and the genetic basis of the mutation are discussed.
Key wordsmurine histocompatibility antigens H-2 mutants genetic recombination structural alterations
Unable to display preview. Download preview PDF.
- Ajitkumar, P., Geier, S. S., Kesari, K. V., Borriello, F., Nakagawa, M., Bluestone, J. A., Saper, M. A., Wiley, D. C., and Nathenson, S. G. (1988). Evidence that multiple residues on both the α-helices of the class I MHC molecule are simultaneously recognized by the T cell receptor.Cell 5447.CrossRefPubMedGoogle Scholar
- Bailey, D. W., Snell, G. D., and Cherry, M. (1971). Complementation and serological analysis of an H-2 mutant. In Lengerova, A., and Vojtiskova, M. (eds.), Proc. Symp. Immunogenet. H-2 Syst., S. Karger AG, Basel, pp. 155–162.Google Scholar
- Geliebter, J., Zeff, R. A., Melvold, R. W., and Nathenson, S. G. (1986b). Mitotic recombination in germ cells generates two MHC mutant genes determined to be identical by RNA sequence analysis: Kbm9 and Kbm6 mutation.Proc. Natl. Acad. Sci. 83371.Google Scholar
- Melvold, R. W., and Kohn, H. I. (1990). Spontaneous frequency of H-2 mutations. In:Transgenic Mice and Mutants in MHC Research Egorov, I. K., and David, C. S. (eds.), Springer-Verlag, Berlin, pp. 3–13.Google Scholar
- Yamaga, K. M., Pfaffenbach, G. M., Pease, L. R., McGovern, D., Nisizawa, T., Melvold, R. W., Kohn, H. I., and Nathenson, S. G. (1983a). Biochemical studies of H-2K antigens from a group of related mutants. I. Identification of a shared mutation in B6-H-2bm5 and B6-H-2bm16.Immunogenetics 1719.CrossRefPubMedGoogle Scholar
- Yamaga, K. M., Pfaffenbach, G. M., Pease, L. R., McGovern, D., Nisizawa, T., Melvold, R. W., Kohn, H. I., and Nathenson, S. G. (1983b). Biochemical studies of H-2K antigens from a group of related mutants. II. Identification of a shared mutation in B6-H-2bm6, B6.C-H-2bm7 and B6.C-H-2bm9.Immunogenetics 1731.CrossRefPubMedGoogle Scholar