Genetic mapping and protein product diversity of the self-incompatibility locus in wild tomato (Lycopersicon peruvianum)
- 25 Downloads
Phenotypic diversity of self-incompatibility (S) alleles within nine natural populations ofLycopersicon peruvianum was investigated. Only 7 incompatible responses were observed of a total of 276 unique combinations tested, on the basis of controlled pollinations, indicating the large number of alleles that exist within these populations. Molecular weight polymorphism for specific major stylar proteins observed on SDS-PAGE was also evident in two of the populations examined. Five proteins were shown to map to theS locus and to be associated with differentS alleles through controlled pollinations and segregation of the proteins. Two of theseS related proteins had been described previously in terms of spatial and temporal expression consistent with their involvement in self-incompatibility (Mauet al., Planta169, 184–191, 1986). A mapping population derived from a fully compatible cross was used to establish linkage of theS locus to two DNA markers,CD15 andTG184, that lie on chromosome 1. The order of the markers and estimates of map distances are given.
Key wordsself-incompatibility (SI) S alleles tomato Lycopersicon peruvianum restriction fragment length polymorphism (RFLP) tomato chromosome 1
Unable to display preview. Download preview PDF.
- Anderson, M. A., Cornish, E. C., Mau, S.-L., Williams, E. G., Hoggart, R., Atkinson, A., Bonig, I., Grego, B., Simpson, R., Roche, P. J., Haley, J. D., Pennschow, J. D., Niall, H. D., Tregear, G. W., Coghlan, J. P., Crawford, R. J., and Clarke, A. E. (1986). Cloning of a cDNA for a style glycoprotein associated with expression of self-incompatibility inNicotiana alata.Nature 32138.Google Scholar
- Atwood, S. S. (1944). Oppositional alleles in natural populations ofTrifolium repens.Genetics 29428.Google Scholar
- Bernatzky, R., and Schilling, A. (1992). Methods for Southern blotting and hybridization. In Osborn, T. C., and Beckmann, J. S. (eds.),Plant Genomes: Methods for Genetic and Physical Mapping Kluwer, The Netherlands.Google Scholar
- Bernatzky, R., and Tanksley, S. D. (1986a). Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences.Genetics 112887.Google Scholar
- Campbell, J. M., and Lawrence, M. J. (1981). The population genetics of the self-incompatibility polymorphism inPapaver rhoeas. I. The number and distribution ofS-alleles in families from three localities.Heredity 4669.Google Scholar
- de Nettancourt, D. (1977).Incompatibility in Angiosperms Springer-Verlag, New York.Google Scholar
- Emerson, S. (1939). A preliminary survey of theOenethera organesis population.Genetics 24538.Google Scholar
- Leach, C. R. (1988). Detection and estimation of linkage for a co-dominant structural gene locus linked to a gametophytic self-incompatibility locus.Theor. Appl. Genet. 75882.Google Scholar
- Miller, J. C., and Tanksley, S. D. (1990). RFLP analysis of phyogenetic relationships and genetic variation in the genusLycopersicon.Theor. Appl. Genet. 80437.Google Scholar
- Rick, C. M. (1986). Reproductive isolation in theLycopersicon peruvianum complex. In D'arcy, W. G. (ed.),Solanaceae Biology and Systematics Columbia University Press, New York, pp. 477–495.Google Scholar
- Tanksley, S. D., Ganal, M. W., Prince, J. P., de Vicente, Bonierbale, M. W., Broun, P., Fulton, T. M., Giovanonni, Grandillo, S., Martin, G. B., Messeguer, R., Miller, J. C., Miller, L., Paterson, A. H., Pineda, O., Röder, M. S., Wing, R. A., Wu, W., and Young, N. D. (1992). High density molecular linkage maps of the tomato and potato genomes: Biological inferences and practical applications.Genetics 1321141–1160.PubMedGoogle Scholar