Skip to main content
SpringerLink
Log in

Chromosomal effects on peptidase activities inDrosophila melanogaster

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

The peptidase system inDrosophila melanogaster (dipeptidase-A, -B, and -C and leucine aminopeptidases G and P) was used as a model to study the effects of modifier genes on activity of enzymes with similar functions. A screen of X, second, and third chromosome substitution isogenic lines revealed the presence of activity modifiers for peptidases on all three chromosomes. Correlation analyses indicated that covariation between some of the peptidase activities is independent of genetic background, while others are associated with variable second chromosomes. Chromosome-specific effects onK m ,V max, and specific activity of partially purified peptidases were also detected. Moreover, a repeatable technique using anion-exchange column chromatography allowed the characterization of possibly two putative peptidic enzymes, glycyl-l-isoleucine-ase andl-leucyl-l-proline-ase, whose kinetic properties differ from the dipeptidases and the leucine aminopeptidases. These findings confirm the existence of activity modifiers for peptidases, much like other enzymes inDrosophila melanogaster.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baker, B. S. (1973). The maternal and zygotic control of development bycinnamon, a new mutant inDrosophila melanogaster.Dev. Biol. 33429.

    Article  CAS  PubMed  Google Scholar 

  • Beckman, L., and Johnson, F. M. (1964). Genetic control of aminopeptidases inDrosophila melanogaster.Heredites 51221.

    Article  CAS  Google Scholar 

  • Bentley, M. M., and Williamson, J. H. (1979). The control of aldehyde oxidase and xanthine dehydrogenase activities by thecinnamon gene inDrosophila melanogaster.Can. J. Genet. Cytol. 21457.

    CAS  Google Scholar 

  • Bentley, M. M., and Williamson, J. H. (1982). The control of aldehyde oxidase and xanthine dehydrogenase activities and CRM levels by themal locus inDrosophila melanogaster.Can. J. Genet. Cytol. 2411.

    CAS  PubMed  Google Scholar 

  • Bentley, M. M., and Williamson, J. H., and Oliver, M. J. (1981). The effects of molybdate, tungstate andlxd on aldehyde oxidase and xanthine dehydrogenase inDrosophila melanogaster.Can. J. Genet. Cytol. 23597.

    CAS  PubMed  Google Scholar 

  • Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.Anal. Biochem.72248.

    Article  CAS  PubMed  Google Scholar 

  • Britten, R. J., and Davidson, E. H. (1969). Gene regulation for higher cells: A theory.Science 165349.

    CAS  PubMed  Google Scholar 

  • Clark, A. G. (1989). Causes and consequences of variation in energy storage inDrosophila melanogaster.Genetics 123131.

    CAS  PubMed  Google Scholar 

  • Collett, J. I. (1976a). Small peptides, a life-long store of amino acid in adultDrosophila andCalliphora.J. Insect Physiol. 221433.

    Article  CAS  Google Scholar 

  • Collett, J. I. (1976b). Peptidase-mediated storage of amino acids in small peptides.Insect Biochem. 6179.

    Article  CAS  Google Scholar 

  • Collett, J. I. (1976c). The constancy and similarity of the amounts of free amino acids in inbred strains ofDrosophila and outbredCalliphora.J. Insect Physiol. 221251.

    Article  CAS  PubMed  Google Scholar 

  • Collett, J. I. (1989). Characterization of the peptidases ofCalliphora: Many features allow the utilization of small peptides as an amino acid reservoir.Insect Biochem. 19535.

    Article  CAS  Google Scholar 

  • Davidson, E. H., and Britten, R. J. (1979). Regulation of gene expression: Possible role of repetitive sequences.Science 2041052.

    CAS  PubMed  Google Scholar 

  • Falke, E. V., and MacIntyre, R. J. (1966). The genetic localization of a non-specific Leucine aminopeptidase inDrosophila melanogaster.Dros. Inform. Serv. 41169.

    Google Scholar 

  • Finnerty, V. (1976). Genetic units ofDrosophila—simple cistrons. In Ashburner, M., and Novitski, E. (eds.),The Genetics and Biology of Drosophila, Vol. 1b Academic Press, New York, p. 721.

    Google Scholar 

  • Freund, R. J., and Littell, R. C. (1981).SAS for Linear Models SAS Institute, Cary, N.C.

    Google Scholar 

  • Gould, S. J. (1977).Ontogeny and Phylogeny Harvard University Press, Cambridge, Mass.

    Google Scholar 

  • Gromnicki, A. R., and Bentley, M. M. (1991). The isolation and characterization of mutant alleles at a new X-linked locus,mex, affecting NADP(+)-dependent enzymes inDrosophila melanogaster.Biochem. Genet. 28145.

    Article  Google Scholar 

  • Hall, N. A. (1986). Peptidases inDrosophila melanogaster. I. Characterization of dipeptidase and leucine aminopeptidase activities.Biochem. Genet. 24775.

    Article  CAS  PubMed  Google Scholar 

  • Hall, N. A. (1988a). Peptidases inDrosophila melanogaster. II. The variation of peptidase activities during development.Insect Biochem.18145.

    Article  CAS  Google Scholar 

  • Hall, N. A. (1988b). Peptidases inDrosophila melanogaster. III. The regulation of leucine aminopeptidase P and leucine aminopeptidase G.Insect Biochem.18157.

    Article  CAS  Google Scholar 

  • Hiraizumi, K., and Laurie, C. C. (1988). Genetic characterization of dipeptidase activity modifiers inDrosophila melanogaster from natural populations.Biochem. Genet. 26783.

    CAS  PubMed  Google Scholar 

  • Hiraizumi, K., Tavormina, P. A., and Mathes, K. D. (1992a). Genetic and environmental effects on the expression of peptidases and larval viability inDrosophila melanogaster.Genetics 131625.

    CAS  PubMed  Google Scholar 

  • Hiraizumi, K., Hourani, C. L., Zambarano, M. C., Freeman, J. E., IV, and Mathes, K. D. (1992b). Dipeptidase-C inDrosophila melanogaster: Genetic, ontogenetic, and tissuespecific variation.Biochem. Genet. 30 (in press).

  • Laurie-Ahlberg, C. C. (1982). Genetic, ontogenetic, and tissue-specific variation of dipeptidases inDrosophila melanogaster.Biochem. Genet. 20407.

    Article  CAS  PubMed  Google Scholar 

  • Laurie-Ahlberg, C. C. (1985). Genetic variation affecting the expression of enzyme-coding genes inDrosophila: An evolutionary perspective. In Rattazzi, M. C. Scandalios, J. G., and Whitt, G. S. (eds.),Isozymes: Current Topics in Biological and Medical Research, Vol. 12 Alan R. Liss, New York, p. 33.

    Google Scholar 

  • Laurie-Ahlberg, C. C., Maroni, G., Bewley, G. C., Lucchessi, J. C., and Weir, B. S. (1980). Quantitative genetic variation of enzyme activities in natural populations ofDrosophila melanogaster.Proc. Natl. Acad. Sci. USA 771073.

    CAS  PubMed  Google Scholar 

  • Lineweaver, H., and Burk, D. (1934). The determination of enzyme dissociation constants.J. Am. Chem. Soc. 56658.

    Article  CAS  Google Scholar 

  • Miyashita, N., Laurie-Ahlberg, C. C., Wilton, A. N., and Emigh, T. H. (1986). Quantitative analysis of X chromosome effects on the activities of the glucose 6-phosphate and 6-phosphogluconate dehydrogenases ofDrosophila melanogaster.Genetics 113321.

    CAS  PubMed  Google Scholar 

  • Mode, C. J., and Robinson, H. F. (1959). Pleiotropism and the genetic variance and covariance.Biometrics 15518.

    Google Scholar 

  • Ohnishi, S., and Voelker, R. A. (1981). Comparative studies of allozyme loci inDrosophila simulans andDrosophila melanogaster. I. Three dipeptidase loci.Biochem. Genet. 1975.

    Article  CAS  PubMed  Google Scholar 

  • Sakai, R. K., Tung, D. A., and Scandalios, J. G. (1969). Developmental genetic studies of aminopeptidases inDrosophila melanogaster.Mol. Gen. Genet. 10524.

    Article  CAS  PubMed  Google Scholar 

  • Satterthwaite, F. E. (1946). An approximate distribution of estimates of variance components.Biomet. Bull. 2110.

    CAS  Google Scholar 

  • Voelker, R. A., and Langley, C. H. (1978). Dipeptidase-A: A polymorphic locus inDrosophila melanogaster.Genetica 49233.

    Article  CAS  Google Scholar 

  • Watanabe, Y., Kumagai, Y., Kubo, Y., Shimamori, Y., and Fujimoto, Y. (1989). Aminopeptidases in human retroplacental sera: Purification and characterization of two enzymes.Biochem. Med. Met. Bio. 41139.

    Article  CAS  Google Scholar 

  • Wilcox, D. R., and Prakash, S. (1980). Variation in biochemical properties of allozymes of xanthine dehydrogenase inDrosophila pseoobscura.Genetics 96927.

    CAS  PubMed  Google Scholar 

  • Wilson, A. C. (1975). Evolutionary importance of gene regulation.Stadler Genet. Symp. 7117.

    CAS  Google Scholar 

  • Wilson, A. C. (1976). Gene regulation in evolution. In Ayala, F. J. (ed.),Molecular Evolution Sinauer Associates, Sunderland, Mass., p. 225.

    Google Scholar 

  • Wilton, A. N., Laurie-Ahlberg, C. C., Emigh, T. H., and Curtsinger, J. W. (1982). Naturally occurring enzyme activity variation inDrosophila melanogaster. II. Relationships among enzymes.Genetics 102207.

    CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Karen D. Mathes

    Present address: ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, 21702, Frederick, Maryland

  2. Christo I. Shalish

    Present address: Molecular Neurogenetics Unit (Neuroscience Center), Massachusetts General Hospital East, 02129, Charleston, Massachusetts

Authors and Affiliations

  1. Department of Biology, Gettysburg College, 17325, Gettysburg, Pennsylvania

    Kazuo Hiraizumi

Authors
  1. Kazuo Hiraizumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karen D. Mathes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christo I. Shalish
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

These studies were supported by grants from the National Institutes of Health (GM42-115-01A1), the Whitaker Foundation of the Research Corporation (C-2560), and the National Science Foundation (USE 8951018) to Kazuo Hiraizumi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hiraizumi, K., Mathes, K.D. & Shalish, C.I. Chromosomal effects on peptidase activities inDrosophila melanogaster . Biochem Genet 31, 29–50 (1993). https://doi.org/10.1007/BF02399818

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02399818

Key words

Search

Navigation