Biochemical Genetics

, Volume 30, Issue 11–12, pp 625–634 | Cite as

Analysis of isozyme loci in the face fly,Musca autumnalis DeGeer

  • E. S. Krafsur
  • W. C. BlackIV


Vertical polyacrylamide gel electrophoresis was used to resolve enzymes at 50 putative loci in Iowa face flies, a recently arrived, colonizing, Palearctic species. Sixty-two percent of the 50 loci were polymorphic. The mean number of alleles per polymorphic locus was 3.2±1.38; the mean among all loci was 2.38±1.62 alleles. The effective number of alleles among 50 loci was 1.4±0.62. Mean observed and expected heterozygosities for the 50 face fly loci were 0.167±0.037 and 0.186±0.031, respectively. Comparison of the electrophoretic data forMusca autumnalis and forM. domestica L. showed similar high levels of gene diversity. A survey of gene diversity at 12 loci among six geographically independent laboratory colonies demonstrated statistically significant genetic differentiation that was probably due to drift after colonization.

Key words

Musca autumnalis Muscidae isozymes genetic diversity breeding structure 


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  1. Black, W. C., and Krafsur, E. S. (1985). Electrophoretic analysis of genetic variability in the house fly (Musca domestica L.).Biochem. Genet. 23193.CrossRefPubMedGoogle Scholar
  2. Bryant, E. H., and Turner, C. R. (1978). Comparative morphometric adaptation of the housefly and the face fly in the United States.Evolution 32759.Google Scholar
  3. Bryant, E. H., van Dijk, H., and van Delden, W. (1981). Genetic variability of the face fly,Musca autumnalis DeGeer, in relation to a population bottleneck.Evolution 35872.Google Scholar
  4. Clayton, J. W., and Tretiak, D. N. (1972). Amine-citrate buffers for pH control in starch gel electrophoresis.J. Fish. Res. Rd. Can. 291169.Google Scholar
  5. Depner, K. R. (1969). Distribution of the face fly,Musca autumnalis (Diptera, Muscidae), in western Canada and the relation between its environment and population density.Can. Entomol. 10197.CrossRefGoogle Scholar
  6. Graur, D. (1985). Gene diversity in Hymenoptera.Evolution 39190.Google Scholar
  7. Krafsur, E. S., Moon, R. D., and Church, C. J. (1985a). Age structure and reproductive history of some overwintering face fly (Diptera: Muscidae) populations in North America.Ann. Entomol. Soc. Am. 78480.Google Scholar
  8. Krafsur, E. S., Moon, R. D., and Kaya, H. K. (1985b). Age structure of breeding face fly populations (Diptera: Muscidae).Environ. Entomol. 14590.Google Scholar
  9. Krafsur, E. S., Helm, J. M., and Black, W. C., IV (1992). Genetic diversity at electrophoretic loci in the house fly,Musca domestica L.Biochem. Genet. 30317.CrossRefPubMedGoogle Scholar
  10. Li, C. C., and Horvitz, D. G. (1953). Some methods of estimating the inbreeding coefficient.Am. J. Hum. Genet. 5107.PubMedGoogle Scholar
  11. Murphy, R. W., Sites, J. W., Buth, D. G., and Haufler, C. H. (1990). Proteins I: Isozyme electrophoresis. In Hillis, D. M., and Moritz, C. (eds.),Molecular Systematics Sinauer Associates, Sunderland, Mass.Google Scholar
  12. Nei, M. (1987).Molecular Population Genetics and Evolution Columbia University Press, New York.Google Scholar
  13. Nei, M., Maruyama, T., and Chakraborty, R. (1975). The bottleneck effect and genetic variability in populations.Evolution 291.Google Scholar
  14. Singh, R. S., and Rhomberg, L. R. (1987). A comprehensive study of genic variation in natural populations ofDrosophila melanogaster. I. Estimates of heterozygosity and patterns of geographical differentiation.Genetics 117255.PubMedGoogle Scholar
  15. Southwood, T. R. E., May, R. M., Hassell, M. P., and Conway, G. R. (1974). Ecological strategies and population parameters.Am. Nat. 108791.CrossRefGoogle Scholar
  16. Swofford, D. L., and Selander, R. B. (1981). BIOSYS-1: A FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics.J. Hered. 72281.Google Scholar
  17. Taylor, F., and Karban, R. (1986).The Evolution of Insect Life Cycles Springer-Verlag, New York.Google Scholar
  18. Thomas, J. M., and Hodes, M. E. (1981). A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH.Anal. Biochem. 118194.CrossRefPubMedGoogle Scholar
  19. Vockeroth, J. R. (1953).Musca autumnalis DeG. in North America (Diptera: Muscidae).Can. Entomol. 85422.CrossRefGoogle Scholar
  20. Weir, B. S., and Cockerham, C. C. (1984). EstimatingF-statistics for the analysis of population structure.Evolution 381358.Google Scholar

Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • E. S. Krafsur
    • 1
  • W. C. BlackIV
    • 2
  1. 1.Department of EntomologyIowa State UniversityAmes
  2. 2.AIDL, Foothills CampusColorado State UniversityFort Collins

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