Mapping of mouse gamma crystallin genes on chromosome 1
- 19 Downloads
Restriction fragments analysis of DNA from mouse-hamster somatic-cell hybrid clones revealed that a mouse gamma crystallin cDNA hybridized to genomic sequences located on mouse chromosome 1. Identification of restriction fragment length polymorphisms (RFLPs) in the gamma crystallin sequences of inbred strains of mice permitted the further localization of the gamma crystallin genes (Cryg) to the proximal region of chromosome 1 closely linked to the loci encoding isocitrate dehydrogenase (Idh-1), a low molecular weight (LM) crystallin protein polymorphism (Len-1), and fibronectin (Fn-1). A single recombinant was observed betweenLen-1 and an RFLP in the gamma crystallin gene family, consistent with the hypothesis thatLen-1 is one of the several structural loci encoding gamma crystallin genes.Len-1 is probably located on the centromeric end of theCryg gene family. Linkage ofIdh-1, Cryg, andFn-1 in mice extends the syntenic relationship of those loci to the human, bovine, and rodent genomes and may define a chromosomal region that is generally conserved among mammals. The map position ofCryg, near the eye lens obsolescence (Elo) locus, was confirmed by the discovery that the restriction fragment patterns of gamma crystallin sequences differed between strain C3H/HeJ and the congenic anophthalmic mutant strain, C3H.Elo. Therefore, the gamma crystallin genes were contransferred with the mutantElo gene in the derivation of C3H.Elo. The results establish that LEN-1 is a marker for the gamma crystallin gene family, position the gamma crystallin gene family relative to other markers on mouse chromosome 1, and provide additional evidence that theElo mutation is encoded at a locus closely linked to the gamma crystallin gene cluster. This study found no evidence of recombination hot spots within the gamma crystallin gene cluster.
Key wordsmouse gamma crystallin gene mapping
Unable to display preview. Download preview PDF.
- Bailey, D. W. (1971). Recombinant inbred strains. An aid to finding identity, linkage and function of histocompatibility and other genes.Transplantation 113254.Google Scholar
- Henry, I., Jeanpierre, M., Bernard, M., Weil, D., Grzschik, K. H., Ramirez, F., Chu, M. L., and Junien, C. (1985). The structural gene for fibronectin (FN) maps to 2p32.3-qter.Cytogenet. Cell. Genet. 40650.Google Scholar
- Holmes, R. S., Mather, P. B., and Duley, J. (1985). Gene markers for alcohol-metabolizing enzymes among recombinant inbred strains of mice with differential behavioral responses towards alcohol.Animal Blood Grps. Biochem. Genet. 1651.Google Scholar
- Lalley, P. A., Francke, U., and Minna, J. D. (1982). Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p.Proc. Natl. Acad. Sci. USA 752382.Google Scholar
- Taylor, B. A. (1978). Recombinant inbred strains: Use in gene mapping. In Morse, H. C. (ed.),Origins of Inbred Mice Academic Press, New York, pp. 423–438.Google Scholar