Biochemical Genetics

, Volume 26, Issue 7–8, pp 463–474 | Cite as

An allele of theProt locus in maize is a variant for the site of protein processing

  • John C. Osterman
Article

Abstract

An allele of theProt locus, which encodes a major globulin of the maize scutellum, is a variant for a site of protein processing. Segregation analysis and recombination mapping indicate that the variant is an allele of theProt locus. DesignatedProt-V, this allele specifies three polypeptides, V1, V2, and V3. The V1 polypeptide is incompletely processed during the proteolytic processing step catalyzed by the product of theMep locus. Cyanogen bromide cleavage studies support the precursor-product relationship between V1 and V2. The V1 product is shortened with respect to other PROT′ proteins and it is postulated that the normal site of MEP processing has been removed by this foreshortening.

Key words

protein processing globulins embryo storage protein Zea mays 

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Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • John C. Osterman
    • 1
  1. 1.School of Biological SciencesUniversity of Nebraska—LincolnLincoln

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