Agarose gel electrophoresis in isozyme separation and visualization
- 60 Downloads
Isozyme analysis of rodent-human somatic cell hybrids has been used frequently to detect specific human chromosomes. The majority of these isozyme systems employs starch gels, the use of which can be laborious when screening large numbers of cell lines. We describe the development of two procedures to detect the long arms of human chromosomes 1 and 2 in Chinese hamster-human cell hybrids by a rapid and reproducible method using 1-mm-thick agarose gels. Detection of human chromosome 1q was accomplished by screening for human fumarate hydratase activity, whose gene has been mapped to 1q42.1. Detection of chromosome 2q was performed by screening for the isozyme isocitrate dehydrogenase 1, which has been localized to 2q32-qter. These systems provide a basis for the further development of procedures for detecting chromosome-specific isozyme markers in agarose gels.
Key wordsisozyme analyses agarose gel electrophoresis chromosome 1 chromosome 2 somatic cell hybrids
Unable to display preview. Download preview PDF.
- Brewer, G. J. (1970).An Introduction to Isozyme Techniques Academic Press, New York.Google Scholar
- Ephrussi, B. (1972).Hybridization of Somatic Cells Princeton University Press, Princeton, NJ.Google Scholar
- Hamerton, J. L. (1975). Report on the committee on the genetic constitution of chromosomes 1,2 (HGM3).Cytogenet. Cell Genet. 167.Google Scholar
- Harris, H. (1971). Cell fusion and the analysis of malignancy.Proc. R. Soc. Lond. Biol. Sci. 1791.Google Scholar
- O'Brien, S. J., Simonson, J. M., and Eichelberger, M. (1982). Genetic analysis of hybrid cells using isozyme markers as monitors of chromosome segregation. In Shay, J. W. (ed.),Techniques in Somatic Cell Genetics Plenum Press, New York, p. 513.Google Scholar