, Volume 108, Issue 1, pp 25–31 | Cite as

The influence of mineral salts on fecundity of the water flea (Daphnia magna) and the implications on toxicity testing of industrial wastewater

  • Gerald A. Leblanc
  • Donald C. Surprenant


The effects of mineral salts constituting water hardness on fecundity ofDaphnia magna were assessed. Of the salts tested, increased concentrations of NaHCO3 and MgSO4 had no effect on fecundity, CaSO4 significantly increased fecundity, and KCl significantly reduced fecundity. The number of offspring produced per daphnid was correlative to the CaSO4 concentration at CaSO4 concentrations between 91 and 2100 mg/ℓ. The effects of CaSO4 on daphnid fecundity could influence the interpretive outcome of industrial wastewater toxicity tests using this species when the waste and dilution waters contain different concentrations of CaSO4. It is recommended that when performing these tests, dilution water be sampled at the intake site of the industry's water source, thus assuring initial comparability of the waste and dilution waters. The CaSO4 content of the water prior to and after industrial use should be determined to identify any alterations of CaSO4 concentration during use. Identification of CaSO4 concentration differences can aid in the interpretation of effects associated with the wastewater.


Daphnia magna water hardness mineral salts fecundity toxicity wastewater 


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  1. Betram, P. E. & Hart, B. A., 1979. Longevity and reproduction of Daphnia pulex (deGeer) exposed to cadmium-contaminated food or water. Envir. Pollut.: 295–305.Google Scholar
  2. Dewey, J. E. & Parker, B. L., 1964. Mass rearing of Daphnia magna for insecticide bioassay. J. econ. Ent. 57: 821–825.Google Scholar
  3. Geiger, J. G., Buikema, A. L. & Cairns, J., 1980. A tentative sevenday test for predicting effects of stress on populations of Daphnia pulex. In: Eaton, J. G., P. R. Parrish & A. C. Hendricks (eds), Aquatic Toxicology. ASTM STP707: 13–26.Google Scholar
  4. Gledhill, W. E., Kaley, R. G., Adams, W. J., Hicks, O., Michael, P. R., Saeger, U. W. & LeBlanc, G. A., 1980. An environmental safety assessment of Butyl Benzyl Phthalate. Envir. Sci. Technol. 14: 301–305.Google Scholar
  5. Goulden, C. E., Comotto, R. M., Hendrickson, J. A., Jr., Hornig, L. L. & Johnson, K. L., 1982. Procedures and recommendations for the culture and use of Daphnia in bioassay studies. In: Pearson, J. G., R. B. Foster & W. E. Bishop (eds), Aquatic Toxicology and Hazard Evaluation. ASTM STP766: 139–160.Google Scholar
  6. Lampert, W., 1978. A field study on the dependence of the fecundity of Daphnia spec. on food concentration. Oecologia 36: 262–369.CrossRefGoogle Scholar
  7. LeBlanc, G. A., 1979. Utilization of bacterial colony counters to count early instar water fleas (Daphnia magna). Bull. envir. Contam. Toxicol. 23: 837–839.Google Scholar
  8. Leblanc, G. A., Schoenfeld, D. A. & Surprenant, D. C., 1981. The effects of food concentration, animal interaction, and water volume on survival, growth and reproduction of Daphnia magna under flow-through conditions. Presented at the 6th Annu. Symp. Toxicol., St. Louis, Missouri. Am. Soc. Test Mate.Google Scholar
  9. National Academy of Sciences, National Academy of Engineering, 1973. Water quality criteria 1972. U.S. Government printing office, Washington, D.C.Google Scholar
  10. Parent, S. & Cheetman, R. D., 1980. Effects of acid precipitation on Daphnia magna. Bull. envir. Contam. Toxicol. 25: 298–300.Google Scholar
  11. Schober, V. & Lampert, W., 1977. Effects of sublethal concentrations of the herbicide atrazine on growth and reproduction of Daphnia pulex. Bull. envir. Contam. Toxicol. 17: 269–277.Google Scholar
  12. Steel, R. G. D. & Torrie, J. H., 1960. Principles and procedures of statistics. McGraw-Hill, N.Y., 418 pp.Google Scholar
  13. U.S. EPA, 1975. Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians. Ecol. Res. Ser., 61 pp.Google Scholar
  14. Wesson, L. G., 1932. A modification of the Osborne Mendel salt mixture containing only inorganic constituents. Science 75: 339–340.Google Scholar
  15. Winner, R. W. & Farrell, M. P., 1976. Acute and chronic toxicity of copper to four species of Daphnia. J. Fish. Res. Bd. Can. 33: 1685–1691.Google Scholar

Copyright information

© Dr W. Junk Publishers 1984

Authors and Affiliations

  • Gerald A. Leblanc
    • 1
  • Donald C. Surprenant
    • 1
  1. 1.EG&G, Bionomics, Aquatic Toxicology LaboratoryWarehamUSA

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