Protein release was studied in Escherichia coli cultivations in minimal medium under different conditions. The energy source concentration was oscillating either due to the cultivation technique or due to an applied on/off feed rate concept in fed-batch cultivations. It was found that the magnitude of protein release was dependent on the cultivation technique and the strain. The use of batch technique resulted in highest specific rate of protein release compared to fed-batch cultivations. No dependence of protein release on oscillating glucose concentration could be distinguished with oscillating periods of minutes of carbon starvation. Proteins released by cells acted as foaming agents and caused stabilisation of foam, during cultivation of Escherichia coli grown in minimal medium. Since the total cell protein was reflected in the medium the protein release is considered to be caused by cell lysis. However, only a few dominating proteins were present in the foam.
Foam Feed Rate Batch Cultivation Bioprocess Engineer Molecular Mass Distribution
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Larsson, G.; Törnkvist, M.; Ståhl Wernersson, E.; Trägårdh, C.; Noorman, H.; Emfors, S.-O.: Substrate gradients in bioreactors: Origin and consequences. Accepted for publication in Bioproc. Eng.Google Scholar
Neubauer, P.;Åhman, M.;Törnkvist, M.;Larsson, G.;Enfors, S.-O.: Response of guanosine tetraphosphate to glucose fluctuations in fed-batch cultivations of Escherichia coli. J. Biotech. 43 (1995) 195–204CrossRefGoogle Scholar
Prins, A.;van't Riet, K.: Proteins and surface effects in fermentation: foam, antifoam and mass transfer. TIBTECH 5 (1987) 296–301CrossRefGoogle Scholar
Hall, M.J.;Dickinson, S.D.;Pritchard, R.;Evans, J.I.: Foams and foam control in fermentation processes. Prog. Ind. Microb. 12 (1973) 169–234Google Scholar
Kalischewski, K.;Bumbullis, W.;Schügerl, K.: Foam behaviour of biological media, I Protein Foams. Europ. J. Appl. Microbiol. Biotechn. 7 (1979) 21–31CrossRefGoogle Scholar
Bumbullis, W.;Kalischewski, K.;Schügerl, K.: Foam behaviour of biological media, II Salt Effects. Europ. J. Appl. Microbiol. Biotechn. 7 (1979) 147–154CrossRefGoogle Scholar
König, B.;Kalischewski, K.;Schügerl, K.: Foam behaviour of biological media, III Penicillium chrysogenum Cultivation Foam. Europ. J. Appl. Microbiol. Biotechn. 7 (1979) 251–258CrossRefGoogle Scholar
Buchholz, H.;Kalischewski, K.;Schügerl, K.: Foam behaviour of biological media, IV Hansenula polymorpha Cultivation Foams. Europ. J. Appl. Microbiol. Biotechn. 7 (1979) 321–331CrossRefGoogle Scholar
Bumbullis, W.;Schügerl, K.: Foam behaviour of biological media, V Alcohol Effects, Europ. J. Appl. Microbiol. Biotechn. 8 (1979) 17–25CrossRefGoogle Scholar
Bumbullis, W.;Schügerl, K.: Foam behaviour of biological media, VI Foam Stability. Salt effects. Europ. J. Appl. Microbiol. Biotechn. 11 (1981) 106–109CrossRefGoogle Scholar
Bumbullis, W.;Kalischewski, K.;Schügerl, K.: Foam behaviour of biological media, VII Surface viscosity and viscoelasticity. Europ. J. Appl. Microbiol. Biotechn. 11 (1981) 110–115CrossRefGoogle Scholar
Morrissey, J.: Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117 (1981) 307–310CrossRefGoogle Scholar
Chuba, P.;Palchaudhuri, S.: Requirement for cysteine in the color silver staining of proteins in polyacrylamide gels. Anal. Biochem. 156 (1985) 136–139CrossRefGoogle Scholar
Schnepf, R.W.;Gaden, Jr E.L.: Foam fractionation of proteins: Concentration of aqueous solutions of bovine serum albumin. J. Biochem. Microbiol. Techn. Eng. Vol 1, no 1 (1959) 1–8CrossRefGoogle Scholar
Manson, C.A.;Hamer, G.;Bryers, J.D.: The death and lysis of microorganisms in environmental processes. FEMS Microbiol Reviews 39 (1986) 373–401CrossRefGoogle Scholar