Abstract
The purpose of this study was to investigate how barley cultivars which are different in dry matter yield at low phosphorus (P) supply (i.e. they differ in agronomic P efficiency) respond to mycorrhizal infection. In a preliminary experiment, six mycorrhizal fungi were tested for their ability to colonize barley (Hordeum vulgare L.) roots at a soil temperature of 15°C.Glomus etunicatum was the most effective species and was used in the main experiment. The main experiment was conducted under glasshouse conditions in which soil temperature was maintained at 15°C. Treatments consisted of a factorial arrangement of 8 barley cultivars, 2 mycorrhiza (inoculated and non-inoculated), and 3 rates of P (0, 10 and 20 mg kg-1). P utilization efficiency (dry matter yield per unit of P taken up) and agronomic P efficiency among the barley cultivars was significantly negatively correlated with mycorrhizal responses. However, the response to mycorrhizal infection was positively correlated with response to P application. Poor correlation was observed between P concentration when neither mycorrhiza nor P were supplied and the percentage of root length infected. The extent of mycorrhizal infection among the barley cultivars in soil without P amendment varied from 8.6 to 28.6%. Significant interactions between cultivar and P addition, and between mycorrhiza and P addition were observed for shoot dry weight but not root dry weight.
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References
Baon J B, Smith S E, Alston A M and Wheeler R D 1992 Phosphorus efficiency of three cereals as related to indigenous mycorrhizal infection. Aust. J. Agric. Res. 43, 479–491.
Baylis G T 1982 Fungi, phosphorus and the evolution of root systems. Search 3, 256–258.
Bowen G D 1991 Soil temperature, root growth and plant function.In Plant Roots. The Hidden Half. Eds. YWaisel, AEshel and UKafkafi. pp 309–330. Marcel Dekker, New York.
Clark R B 1990 Physiology of cereals for mineral nutrient uptake, use and efficiency.In Crops as Enhancers of Nutrient Use. Eds. V CBaligar and R RDuncan. pp 131–209. Academic Press, San Diego, California, USA.
Colwell J D 1963 The estimation of the phosphorus fertilizer requirements of wheat in Southern New South Wales by soil analysis. Aust. J. Exp. Agric. Anim. Husb. 3, 190–197.
Estaún V, Calvet C and Hayman D S 1987 Influence of plant genotype on mycorrhizal infection. Response of three pea cultivars. Plant and Soil 103, 295–298.
Fabig B, Moawad A M and Achnich W 1989 Effect of mycorrhiza on dry weight and phosphorus content in shoots of cereals crops fertilized with rock phosphate at different soil pH and temperature levels. Z. Pflanzenernaehr. Bodenkd. 152, 255–259.
Föhse D, Claassen N and Jungk A 1991 Phosphorus efficiency of plants. 2. Significance of root radius, root hairs and cation-anion balance for phosphorus influx in seven plant species. Plant and Soil 132, 261–270.
Genstat 5 Commitee 1987 GENSTAT 5: Reference Manual 1987. Clarendon Press, Oxford.
Giovannetti M and Mosse B 1980 An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytol. 84, 489–500.
Graham R D 1984 Breeding for nutritional characteristics in cereals.In Advances in Plant Nutrition. Eds. P BTinker and ALaüchli. pp 57–102. Praeger Publisher, New York, USA.
Graham J H and Syvertsen J P 1985 Host determinants of mycorrhizal dependency of citrus stock seedlings. New Phytol. 101, 667–676.
Grey W E 1991 Influence of temperature on colonization of spring barleys by vesicular-arbuscular mycorrhizal fungi. Plant and Soil 137, 181–190.
Hanson W C 1950 The photometric determination of phosphorus in fertilizers using the phosphovanado-molybdate complex. J. Sci. Food Agric. 1, 172–173.
Hetrick B A D, Bockus W W and Bloom J 1984 The role of vesicular-arbuscular mycorrhizal fungi in the growth of Kansas winter wheat. Can. J. Bot. 62, 735–740.
Jensen A 1983 The effect of indigenous vesicular-arbuscular mycorrhizal fungi on nutrient uptake and growth of barley in two Danish soils. Plant and Soil 70, 155–163.
Lyness A S 1936 Varietal differenes in phosphorus feeding capacity of plants. Plant Physiol. 11, 665–688.
Menge J A, Steirle D, Bagyaraj D J, Johnson E L V and Leonard R T 1978 Phosphorus concentrations in plants responsible for inhibition of mycorrhizal infection. New Phytol. 80, 575–578.
Ollivier B, Bertheau Y, Diem H G and Gianinazzi-Pearson V 1983 Influence de la variété deVigna unguiculata dans l'expression de trois associations endomycorrhiziennes à vésicules et arbuscules. Can. J. Bot. 61, 354–358.
Phillips J M and Hayman D S 1970 Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment. Trans. Br. Mycol. Soc. 55, 158–160.
Pearson J N, Smith S E and Smith F A 1991 Effect of photon irradiance on the development and activity of VA mycorrhizal infection in Allium porrum. Myc. Res. 95, 741–746.
Power J F, Grunes D L, Willis W O and Reichman G A 1963 Soil temperature and phosphorus effects upon barley growth. Agron. J. 55, 389–392.
Schjørring J K and Nielsen N E 1987 Root length and phosphorus uptake by four barley cutlivars grown under moderate deficiency of phosphorus in field experiments. J. Plant Nutr. 10, 1289–1295.
Smith S E 1980 Mycorrhizas of autotrophic higher plants. Biol. Rev. 55, 475–510.
Smith S E and Walker N A 1981 A quantitative study of mycorrhizal infection in Trifolium: separate determination of the rates of infection and of mycelial growth. New Phytol. 89, 225–240.
Smith S E, Robson A D and Abbott L K 1992 The involvement of mycorrhizas in assessment of genetically dependent efficiency of nutrient uptake and use. Plant and Soil 146, 169–179.
Soil Survey Staff 1975 Soil Taxonomy — a basic system of soil classification for making and interpreting soil surveys. USDA-SCS Agric. Handb. 436, Washington, D.C.
Stace H T C, Hubble G D, Brewer R, Northcote K H, Sleeman J R, Mulcahy M J and Hallsworth E C 1968 A Handbook of Australian Soils. Rellim: Glenside, S.A.
Stribley D P, Tinker P B and Rayner J H 1980 Relation of internal phosphorus concentration and plant weight in plants infected by vesicular-arbuscular mycorrhizas. New Phytol. 86, 261–266.
Thompson J P 1990 Soil sterilization methods to show VA-mycorrhizae aid P and Zn nutrition of wheat in vertisols. Soil Biol. Biochem. 22, 229–240.
Toth R, Page T and Castleberry R 1984 Differences in mycorrhizal colonization of maize selections for high and low ear leaf phosphorus. Crop Sci. 24, 994–996.
Volkmar K M and Woodbury W 1989 Effects of soil temperature and depth on colonization and root and shoot growth of barley inoculated with vesicular-arbuscular mycorrhizae indigenous to Canadian prairie soil. Can J. Bot. 67, 1702–1707.
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Baon, J.B., Smith, S.E. & Alston, A.M. Mycorrhizal responses of barley cultivars differing in P efficiency. Plant Soil 157, 97–105 (1993). https://doi.org/10.1007/BF02390231
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DOI: https://doi.org/10.1007/BF02390231