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The effect of thyroxine and cortisol on some dehydrogenases and lysosomal enzymes activities in rat pineal cultures

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A histoenzymologic investigation was made concerning the effects of variable doses of thyroxine, cortisol, growth hormone, testosterone propionate and stilboestrol on some oxydo-reducing and lysosomal enzymes activity in rat pineal cell cultures. The thyroxine stimulated predominantly the enzymes of Krebs and Emhden-Meyerhoff cycles and the lipid metabolism. The cortisol stimulated the aryl-sulphatase only. No effect was shown by the pituitary growth and sex hormones. By means of hystoenzymologic techniques two pinealocytes types werein vitro identified: round (basophile) highly active cells and polyedric (chief) cells with lesser enzymatic activity.

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References

  • Fishman, W. H.,R. Abraham, andDolores Delellis: Beta glucuronidase response to androgens as a histochemical model for a hormone-enzyme relation. Ann. Histochim.11, 391–402 (1966).

    CAS  PubMed  Google Scholar 

  • Grignon, M., etG. Grignon: Étude histologique et histophysiologique de l'épiphyse chez la tortue terrestre (Testudo mauritanica). Ann. Endocr. (Paris)24, 356–364 (1963).

    CAS  Google Scholar 

  • Guha, S., etR. Wegmann: Étude sur l'activité phosphorylasique. I. Une nouvelle méthode de mise en évidence de la phosphorylase. Ann. Histoohim.4, 103–114 (1959).

    CAS  Google Scholar 

  • Hungerford, G. F., andC. M. Pomerat: Rat pineal parenchimal cells as observed in tissue culture. Z. Zellforsch.57, 809–817 (1962).

    CAS  PubMed  Google Scholar 

  • Krass, E. M., andF. S. Labella: Hexozomonophosphate shunt in endocrine tissues. Quantitative estimation of the pathway in bovine pineal body, anterior pituitary, posterior pituitary and brain. Biochim. biophys. Acta (Amst.)148, 384–391 (1967).

    CAS  Google Scholar 

  • Lazarus, S. S., andH. Barden: Ultramicroscopic localization of mitochondrial adenosine triphosphatase. J. Ultrastruct. Res.10, 189–193 (1964).

    Article  CAS  PubMed  Google Scholar 

  • Milcu, S. M., E. Holban etE. Ghinea: L'étude morphologique de la pinéale cultivée in vitro. Nat. Congr. Endocrin. Bucharest, 1967, p. 211.

  • E. Ghinea: In vitro study of thyroxine effects on enzymatic activity and cell differentiation in the pineal gland. Rev. Roum. Endocr.5, 203–208 (1968).

    CAS  Google Scholar 

  • Moszkowska, A.: Contribution à l'étude du mécanisme de l'antagonisme épiphyso-hypophysaire. In: Progress in brain research. Structure and function of the epiphysis cerebri (J. Ariens Kappers andJ. P. Schadé eds.), vol. 10. Amsterdam-London-New York: Elsevier 1965.

    Google Scholar 

  • Nachlas, M. M.,D. T. Crawfordt, andA. Seligman: The histochemical demonstration of leuoine aminopeptidase. J. Histochem. Cytochem.5, 264–267 (1957a).

    CAS  PubMed  Google Scholar 

  • S. C. Cheng, andA. M. Seligman: Cytochemioal demonstration of succinic dehydrogenase by the use of a new nitrophenyl substituted ditetrazolium. J. Histochem. Cytochem.5, 420–436 (1957b).

    CAS  PubMed  Google Scholar 

  • A. M. Seligman: A histochemical method for the demonstration of diphosphopyridine nucleotide diaphorase. J. biophys. biochem. Cytol.4, 29–38 (1958a).

    CAS  PubMed  Google Scholar 

  • —The histochemical localization of triphosphopyridine nucleotide diaphorase. J. biophys. biochem. Cytol.4, 467–474 (1958b).

    CAS  PubMed  Google Scholar 

  • A. M. Seligman: Variations in reduction of tetrazolium salts by dehydrogenase systems. Proc. Soc. exp. Biol. (N.Y.)104, 407–409 (1960).

    CAS  Google Scholar 

  • Pearse, A. G. E.: Histochemistry theoretical and applied. London: J. and A. Churchill 1961.

    Google Scholar 

  • Petersson, B.: Cytochemical studies of hydrolytic and oxidative enzymes in sections and isolated cells from the endocrine paneras of guinea pigs. Histochemie7, 116–131 (1966).

    Article  CAS  PubMed  Google Scholar 

  • Rosa, Ch. G., andK. C. Tsotj: The use of tetranitroblue tetrazolium for the cytochemical localization of succinic dehydrogenase. J. Cell Biol.16, 445–454 (1963).

    Article  CAS  PubMed  Google Scholar 

  • Rutenburg, A. M.,R. B. Cohen, andA. M. Seligman: Histochemical demonstration of arylsulphatase. Science116, 539–543 (1952).

    Google Scholar 

  • Scarpelli, D. G.,R. Hess, andA. G. E. Pearse: The cytochemical localization of oxydative enzymes I. DPN diaphorase and TPN diaphorase. J. biophys. biochem. Cytol.4, 747–752 (1958).

    CAS  PubMed  Google Scholar 

  • Seligman, A. M.,K. C. Tsou,S. M. Rutenburg, andR. B. Cohen: Histochemical demonstration of beta glucuronidase with a synthetic substrate. J. Histochem. Cytochem.2, 209–222 (1954).

    CAS  PubMed  Google Scholar 

  • Tasca, C.,E. Ghinea, andO. Stanescu: Histoenzymologic investigations on tissue culture of rat pineal gland. Rev. Roum. Endocrin.5, 21–25 (1968).

    CAS  Google Scholar 

  • Thiéblot, L., etS. Blaise: Influence de la glande pinéale sur la sphère génitale. In: Progress in brain research. Structure and function of the epiphysis cerebri (c eds.), vol. 10. Amsterdam-London-New York: Elsevier 1965.

    Google Scholar 

  • Wachstein, M., andE. Meisel: Histochemistry of hepatic phosphatase at a physiological pH with special reference to the demonstration of bile canaliculi. Amer. J. clin. Path.27, 13–23 (1957).

    CAS  Google Scholar 

  • Wegmann, R., etG. Gerzeli: La glucose-6-phosphate deshydrogénase et ses corrélations avec des substrats voisins du glucose-6-phosphate. Rôle de l'hexokinase. Ann. Histochim.6, 111–124 (1961).

    CAS  PubMed  Google Scholar 

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Milcu, S.M., Petrescu, R. & Tasca, C. The effect of thyroxine and cortisol on some dehydrogenases and lysosomal enzymes activities in rat pineal cultures. Histochemie 15, 312–317 (1968). https://doi.org/10.1007/BF02389696

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