Electron microscopic localization of carbonic anhydrase (CA) activity in rabbit cornea

  • Sadaki Yokota
  • Wolfgang K. Waller
Article

Summary

The localization of CA activity in the rabbit cornea was investigated using a modified Hansson's method. The reaction products indicating CA activity were observed in the lateral membrane of endothelial cell, but not in the apical membrane. In cytoplasm, Golgi complex, and mitochondria of the endothelial cells no specific reaction product could be found. In epithelium, stroma, and Descemet's membrane, no reaction product was seen. The specific staining was inhibited in the control group which was treated with 10−4 M sodium acetazolamide.

Keywords

Endothelial Cell Carbonic Anhydrase Apical Membrane Acetazolamide Golgi Complex 

Zusammenfassung

Die Lokalisation der Carboanhydrase-Aktivität wurde bei Kaninchenhornhäuten mit einer modifizierten Methode nach Hansson untersucht. Spezifische Reaktionsprodukte konnten an den seitlichen Zellmembranen des Endothels beobachtet werden. Im Cytoplasma, im Golgikomplex und in den Mitochondrien waren keine spezifischen Ablagerungen zu finden, ebensowenig im Epithel, im Stroma und in der Descemetschen Membran. Bei einer Kontrollgruppe ließ sich die spezifische Färbung mit 10−4 M Azetazolamid hemmen.

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Sadaki Yokota
    • 1
  • Wolfgang K. Waller
    • 1
  1. 1.Augenklinik der Universität WürzburgWürzburgFederal Republic of Germany

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